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Pretreatment of Asian elephant (Elephas maximus) spermatozoa with cholesterol-loaded cyclodextrins and glycerol addition at 4°C improves cryosurvival

Wendy K. Kiso A F , Atsushi Asano B , Alexander J. Travis B , Dennis L. Schmitt C D , Janine L. Brown E and Budhan S. Pukazhenthi E
+ Author Affiliations
- Author Affiliations

A Center for Species Survival, Smithsonian Conservation Biology Institute, National Zoological Park, Washington, DC 20008, USA.

B Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.

C The William H. Darr School of Agriculture, Missouri State University, Springfield, MO 65897, USA.

D The Ringling Brothers Center for Elephant Conservation, Polk City, FL 33868, USA.

E Center for Species Survival, Smithsonian Conservation Biology Institute, National Zoological Park, Front Royal, VA 22630, USA.

F Corresponding author. Email: wkiso@feldinc.com

Reproduction, Fertility and Development 24(8) 1134-1142 https://doi.org/10.1071/RD11266
Submitted: 15 October 2011  Accepted: 4 April 2012   Published: 3 May 2012



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