Register      Login
Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology

Articles citing this paper

Human spermatozoa vitrified in the absence of permeable cryoprotectants: birth of two healthy babies

Vladimir Isachenko A E , Evgenia Isachenko A , Anna M. Petrunkina B C E and Raul Sanchez D
+ Author Affiliations
- Author Affiliations

A Department of Obstetrics and Gynaecology, University Women Hospital, Kerpener Str. 34, 50931 Cologne, Germany.

B Unit of Reproductive Medicine of Clinics, Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, Buenteweg 15, Hannover 30559, Germany.

C Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK.

D University de La Frontera, Faculty of Medicine, Temuco, Chile.

E Corresponding authors. Emails: v.isachenko@yahoo.com; anna.petrunkina@gmx.de

Reproduction, Fertility and Development 24(2) 323-326 https://doi.org/10.1071/RD11061
Submitted: 26 February 2011  Accepted: 13 May 2011   Published: 13 October 2011



54 articles found in Crossref database.

Ultra-rapid cooling of ibex sperm by spheres method does not induce a vitreous extracellular state and increases the membrane damages
Bóveda Paula, Toledano-Díaz Adolfo, Castaño Cristina, Esteso Milagros Cristina, López-Sebastián Antonio, Rizos Dimitrios, Bielli Alejandro, Ungerfeld Rodolfo, Santiago-Moreno Julián, Yu Yang
PLOS ONE. 2020 15(1). p.e0227946
Technologies of cryoprotectant‐free vitrification of human spermatozoa: asepticity as criterion of effectiveness
Isachenko V., Rahimi G., Mallmann P., Sanchez R., Isachenko E.
Andrology. 2017 5(6). p.1055
Effect of trehalose- and sucrose-based extenders on equine sperm quality after vitrification: Preliminary results
Pérez-Marín C.C., Requena F.D., Arando A., Ortiz-Villalón S., Requena F., Agüera E.I.
Cryobiology. 2018 80 p.62
A comprehensive review and update on human fertility cryopreservation methods and tools
Antonouli Sevastiani, Di Nisio Valentina, Messini Christina, Daponte Alexandros, Rajender Singh, Anifandis George
Frontiers in Veterinary Science. 2023 10
Storage temperature and sucrose concentrations affect ram sperm quality after vitrification
Arando A., Gonzalez A., Delgado J.V., Arrebola F.A., Perez-Marín C.C.
Animal Reproduction Science. 2017 181 p.175
O impacto da criopreservação na qualidade seminal
Cipriano Vivian Taís Fernandes, Freitas Gilberto da Costa
Reprodução & Climatério. 2013 28(3). p.112
Effect of cooling rate on sperm quality of cryopreserved Andalusian donkey spermatozoa
Demyda-Peyrás S., Bottrel M., Acha D., Ortiz I., Hidalgo M., Carrasco J.J., Gómez-Arrones V., Gósalvez J., Dorado J.
Animal Reproduction Science. 2018 193 p.201
Infertility and Assisted Reproduction (2021)
Niu Xiangli, Huang Hua, Mo Yi, Sun Yan, Wang Wei-Hua
Aseptic capillary vitrification of human spermatozoa: Cryoprotectant-free vs. cryoprotectant-included technologies
Wang Mengying, Todorov Plamen, Isachenko Evgenia, Rahimi Gohar, Wang Wanxue, von Brandenstein Melanie, Kumar Pradeep, Mallmann Peter, Isachenko Vladimir
Cryobiology. 2021 99 p.95
Efficiency and Safety of Human Reproductive Cell/Tissue Vitrification
Parmegiani L., Maggiulli R., Menegazzo M., Ebner T.
Journal of Reproductive and Stem Cell Biotechnology. 2012 3(1). p.22
The relationship between reactive oxygen species, DNA fragmentation, and sperm parameters in human sperm using simplified sucrose vitrification with or without triple antioxidant supplementation
Juanpanich Theesit, Suttirojpattana Tayita, Parnpai Rangsun, Vutyavanich Teraporn
Clinical and Experimental Reproductive Medicine. 2022 49(2). p.117
Preservation of boar semen: An update
Pezo Felipe, Romero Fernando, Zambrano Fabiola, Sánchez Raúl Segundo
Reproduction in Domestic Animals. 2019 54(3). p.423
Vitrificación de espermatozoides: una alternativa a la inyección intracitoplasmática de espermatozoides en paciente con oligoastenozoospermia severa
Sánchez Raúl, Schulz Mabel, Risopatrón Jennie, Isachenko Vladimir, Isachenko Evgenia
Revista Internacional de Andrología. 2013 11(1). p.36
In Vitro Fertilization (2019)
Isachenko Vladimir, Rahimi Gohar, Mallmann Peter, Sanchez Raul, Isachenko Evgenia
Improved cryopreservation of spermatozoa using vitrification: comparison of cryoprotectants and a novel device for long-term storage
O’Neill Helen C., Nikoloska Maya, Ho HiuTung, Doshi Alpesh, Maalouf Walid
Journal of Assisted Reproduction and Genetics. 2019 36(8). p.1713
A simple method of human sperm vitrification
Shah Dupesh, Rasappan , Shila , Gunasekaran Karthik
MethodsX. 2019 6 p.2198
Human sperm vitrification: the state of the art
Tao Yong, Sanger Erika, Saewu Arpornrad, Leveille Marie-Claude
Reproductive Biology and Endocrinology. 2020 18(1).
Vitrified sperm banks: the new aseptic technique for human spermatozoa allows cryopreservation at −86 °C
Sánchez R., Risopatrón J., Schulz M., Villegas J. V., Isachenko V., Isachenko E.
Andrologia. 2012 44(6). p.433
Stallion sperm freezing with sucrose extenders: A strategy to avoid permeable cryoprotectants
Consuegra C., Crespo F., Bottrel M., Ortiz I., Dorado J., Diaz-Jimenez M., Pereira B., Hidalgo M.
Animal Reproduction Science. 2018 191 p.85
First pregnancies in jennies with vitrified donkey semen using a new warming method
Diaz-Jimenez M., Rota A., Dorado J., Consuegra C., Pereira B., Camillo F., Panzani D., Fanelli D., Tesi M., Monaco D., Hidalgo M.
Animal. 2021 15(1). p.100097
Laboratory and clinical management of leukocytospermia and hematospermia: a review
Khodamoradi Kajal, Kuchakulla Manish, Narasimman Manish, Khosravizadeh Zahra, Ali Aleena, Brackett Nancy, Ibrahim Emad, Ramasamy Ranjith
Therapeutic Advances in Reproductive Health. 2020 14 p.263349412092251
Human sperm vitrification: A scientific report
Schulz Mabel, Risopatrón Jennie, Uribe Pamela, Isachenko Evgenia, Isachenko Vladimir, Sánchez Raúl
Andrology. 2020 8(6). p.1642
Large volume cryoprotectant-free vitrification: an alternative to conventional cryopreservation for human spermatozoa
Slabbert M., du Plessis S.S., Huyser C.
Andrologia. 2015 47(5). p.594
Cryoprotectant-free vitrification of spermatozoa: Fish as a model of human
Isachenko Vladimir, Sanchez Raul, Rahimi Gohar, Mallmann Peter, Isachenko Evgenia, Merzenich Markus
Andrologia. 2019 51(1). p.e13166
Kinetic vitrification: concepts and perspectives in animal sperm cryopreservation
Barbosa Bianca Barreto, Evangelista Inara Tayná Alves, Soares Airton Renan Bastos, Leão Danuza Leite, Pereira Ricardo José Garcia, Domingues Sheyla Farhayldes Souza
Animal Reproduction. 2023 20(2).
Live Birth After Intrauterine Insemination With Spermatozoa From an Oligoasthenozoospermic Patient Vitrified Without Permeable Cryoprotectants
Sanchez Raul, Isachenko Vladimir, Petrunkina Anna M., Risopatron Jennie, Schulz Mabel, Isachenko Evgenia
Journal of Andrology. 2012 33(4). p.559
Analysis of DNA damage after human sperm cryopreservation in genes crucial for fertilization and early embryo development
Valcarce D. G., Cartón-García F., Riesco M. F., Herráez M. P., Robles V.
Andrology. 2013 1(5). p.723
Effects of vitrification on ram spermatozoa using free-egg yolk extenders
Jiménez-Rabadán Pilar, García-Álvarez Olga, Vidal Ana, Maroto-Morales Alejandro, Iniesta-Cuerda María, Ramón Manuel, del Olmo Enrique, Fernández-Santos Rocío, Garde J. Julián, Soler Ana Josefa
Cryobiology. 2015 71(1). p.85
Unraveling Subcellular and Ultrastructural Changes During Vitrification of Human Spermatozoa: Effect of a Mitochondria-Targeted Antioxidant and a Permeable Cryoprotectant
Kumar Pradeep, Wang Mengying, Isachenko Evgenia, Rahimi Gohar, Mallmann Peter, Wang Wanxue, Brandenstein Melanie von, Isachenko Vladimir
Frontiers in Cell and Developmental Biology. 2021 9
Cryopreservation of Mammalian Gametes and Embryos (2017)
Isachenko Vladimir, Sanchez Raul, Mallmann Peter, Rahimi Gohar, Isachenko Evgenia
Cryopreservation of a small number of human sperm using enzymatically fabricated, hollow hyaluronan microcapsules handled by conventional ICSI procedures
Tomita Kazuhisa, Sakai Shinji, Khanmohammadi Mehdi, Yamochi Takayuki, Hashimoto Shu, Anzai Masayuki, Morimoto Yoshiharu, Taya Masahito, Hosoi Yoshihiko
Journal of Assisted Reproduction and Genetics. 2016 33(4). p.501
Fundamental Principles of Cryobiology and Application toEx situConservation of Avian Species
Liu Jianan, Cheng Kimberly M., Silversides Frederick G.
Avian Biology Research. 2013 6(3). p.187
Biennial Review of Infertility (2015)
Hennet Margo L., Combelles Catherine M. H.
Ultrastructural and Functional Characteristics of Human Spermatozoa After Cryopreservation by Vitrification
Pavlovych Olena, Hapon Hanna, Yurchuk Taisiia, Repin Mykola, Marchenko Larysa, Govorukha Tetiana, Petrushko Maryna
Problems of Cryobiology and Cryomedicine. 2020 30(1). p.24
Effect of cryopreservation on human sperm messenger RNAs crucial for fertilization and early embryo development
Valcarce D.G., Cartón-García F., Herráez M.P., Robles V.
Cryobiology. 2013 67(1). p.84
New permeable cryoprotectant-free vitrification method for native human sperm
Aizpurua J., Medrano L., Enciso M., Sarasa J., Romero A., Fernández M.A., Gómez-Torres M.J.
Human Reproduction. 2017 32(10). p.2007
Conventional slow freezing cryopreserves mouflon spermatozoa better than vitrification
Pradiee J., Esteso M. C., Castaño C., Toledano-Díaz A., Lopez-Sebastián A., Guerra R., Santiago-Moreno J.
Andrologia. 2017 49(3). p.e12629
First birth of a healthy infant following intra-cytoplasmic sperm injection using a new permeable cryoprotectant-free sperm vitrification protocol
Medrano Llanos, Enciso María, Gomez-Torres María José, Aizpurua Jon
Cryobiology. 2019 87 p.117
Protective effect of butylated hydroxytoluene on sperm function in human spermatozoa cryopreserved by vitrification technique
Merino O., Aguagüiña W. E., Esponda P., Risopatrón J., Isachenko E., Isachenko V., Sánchez R.
Andrologia. 2015 47(2). p.186
Description and Outcomes of Current Clinical Techniques for Sperm Cryopreservation
Palomar Rios Andrea, Molina Botella Inmaculada
EMJ Reproductive Health. 2019  p.79
Porcine sperm vitrification II: Spheres method
Arraztoa C. C., Miragaya M. H., Chaves M. G., Trasorras V. L., Gambarotta M. C., Neild D. M.
Andrologia. 2017 49(8). p.e12738
Vitrification of Human ICSI/IVF Spermatozoa Without Cryoprotectants: New Capillary Technology
Isachenko V., Maettner R., Petrunkina A. M., Sterzik K., Mallmann P., Rahimi G., Sanchez R., Risopatron J., Damjanoski I., Isachenko E.
Journal of Andrology. 2012 33(3). p.462
Vitrification of a small number of spermatozoa in normozoospermic and severely oligozoospermic samples
Kuznyetsov Valeriy, Moskovtsev Sergey I., Crowe Michael, Lulat Ayub G.-M., Librach Clifford L.
Systems Biology in Reproductive Medicine. 2015 61(1). p.13
Cryopreservation of human spermatozoa with minimal non-permeable cryoprotectant
Liu Jie, Tanrikut Cigdem, Wright Diane L., Lee Gloria Y., Toner Mehmet, Biggers John D., Toth Thomas L.
Cryobiology. 2016 73(2). p.162
Cryoprotectant-free ultra-rapid freezing of human spermatozoa in cryogenic vials
Zhu J., Jin R.-T., Wu L.-M., Johansson L., Guo T.-H., Liu Y.-S., Tong X.-H.
Andrologia. 2014 46(6). p.642
Freezing, Vitrification, and Freeze-Drying of Equine Spermatozoa: Impact on Mitochondrial Membrane Potential, Lipid Peroxidation, and DNA Integrity
Restrepo Giovanni, Varela Elizabeth, Duque Juan Esteban, Gómez Jorge Enrique, Rojas Mauricio
Journal of Equine Veterinary Science. 2019 72 p.8
Ultra-Rapid Freezing Preserves Morphofunctional Integrity and Fertilizing Ability of Epididymal Cat Spermatozoa
Colombo Martina, Morselli Maria Giorgia, Zahmel Jennifer, Luvoni Gaia Cecilia
Frontiers in Veterinary Science. 2022 9
Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches
Hezavehei Maryam, Sharafi Mohsen, Kouchesfahani Homa Mohseni, Henkel Ralf, Agarwal Ashok, Esmaeili Vahid, Shahverdi Abdolhossein
Reproductive BioMedicine Online. 2018 37(3). p.327
Successful ultrarapid cryopreservation of wild Iberian ibex (Capra pyrenaica) spermatozoa
Pradiee J., Esteso M.C., Lopez-Sebastián A., Toledano-Díaz A., Castaño C., Carrizosa J.A., Urrutia B., Santiago-Moreno J.
Theriogenology. 2015 84(9). p.1513
Conventional freezing vs. cryoprotectant-free vitrification of epididymal (MESA) and testicular (TESE) spermatozoa: Three live births
Spis Emilia, Bushkovskaia Anna, Isachenko Evgenia, Todorov Plamen, Sanchez Raul, Skopets Viola, Isachenko Vladimir
Cryobiology. 2019 90 p.100
Cryopreservation in Assisted Reproduction (2024)
Wang Mengying, Isachenko Evgenia, Rahimi Gohar, Kumar Pradeep, Mallmann Peter, Isachenko Volodimir
Epigenetic Alterations in Cryopreserved Human Spermatozoa: Suspected Potential Functional Defects
Wang Wanxue, Todorov Plamen, Pei Cheng, Wang Mengying, Isachenko Evgenia, Rahimi Gohar, Mallmann Peter, Isachenko Vladimir
Cells. 2022 11(13). p.2110
Spermatozoa Cryopreservation: State of Art and Future in Small Ruminants
Lv Chunrong, Wu Guoquan, Hong Qionghua, Quan Guobo
Biopreservation and Biobanking. 2019 17(2). p.171
Optimization of donkey sperm vitrification: Effect of sucrose, sperm concentration, volume and package (0.25 and 0.5 mL straws)
Diaz-Jimenez M., Dorado J., Consuegra C., Ortiz I., Pereira B., Carrasco J.J., Gomez-Arrones V., Domingo A., Hidalgo M.
Animal Reproduction Science. 2019 204 p.31

Committee on Publication Ethics


Abstract Export Citation Get Permission