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RESEARCH ARTICLE

Assessment of the physicochemical characteristics of surface waterbodies in a region earmarked for shale gas exploration (Eastern Cape Karoo, South Africa)

Annah Mabidi A B C , Matthew S. Bird A and Renzo Perissinotto A
+ Author Affiliations
- Author Affiliations

A South African Research Chairs Initiative, Shallow Water Ecosystems, Nelson Mandela Metropolitan University, PO Box 77000, Port Elizabeth 6031, South Africa.

B Africa Earth Observatory Network, Nelson Mandela Metropolitan University, PO Box 77000, Port Elizabeth 6031, South Africa.

C Corresponding author. Email: annahanusa@gmail.com

Marine and Freshwater Research 68(9) 1626-1641 https://doi.org/10.1071/MF16102
Submitted: 31 March 2016  Accepted: 3 November 2016   Published: 9 January 2017

Abstract

The proposed drilling for shale gas resources in the Eastern Cape Karoo region of South Africa has triggered much debate over the potential effects of hydraulic fracturing on water resources. Herein we present results on some limnological aspects of surface waterbodies in this water-scarce region before shale gas exploration. Thirty-three waterbodies (nine dams, 13 depression wetlands and 11 rivers) were sampled in November 2014 and April 2015. Principal component analysis revealed that depression wetlands and rivers had distinct physicochemical signatures, whereas dams were highly variable in their physicochemical attributes and exhibited characteristics similar to those of either rivers or depression wetlands. Non-parametric multivariate regressions and permutational multivariate analysis of variance (MANOVA) indicated that landscape variables such as underlying geology, altitude and land use poorly explained the physicochemical characteristics of the sampled waterbodies. Waterbody type was the only factor that explained a significant amount of the variation in physicochemistry during both sampling events. These data need to be supplemented by water quality information from additional sites and over longer time periods, as well as supporting data relating to other aspects, such as algae and invertebrates, before they can be used as a baseline for the long-term monitoring of freshwater ecosystems in the region.

Additional keywords: freshwater ecosystems, hydraulic fracturing, semi-arid regions, temporary wetlands, water resource monitoring.


References

Akob, D. M., Cozzarelli, I. M., Dunlap, D. S., Rowan, E. L., and Lorah, M. M. (2015). Organic and inorganic composition and microbiology of produced waters from Pennsylvania shale gas wells. Applied Geochemistry 60, 116–125.
Organic and inorganic composition and microbiology of produced waters from Pennsylvania shale gas wells.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXntFGrt7Y%3D&md5=02771c4be334c01691536143554a37e6CAS |

Aldridge, K. T., Lamontagne, S., Deegan, B. M., and Brookes, J. D. (2011). Impact of a drought on nutrient concentrations in the Lower Lakes (Murray Darling Basin, Australia). Inland Waters 1, 159–176.
Impact of a drought on nutrient concentrations in the Lower Lakes (Murray Darling Basin, Australia).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXhslGku7%2FL&md5=0823f148e0ea3790b9dcc8753fbaf038CAS |

Allanson, B. R., Hart, R. C., O’Keeffe, J. H., and Robarts, R. D. (Eds) (1990). ‘Inland Waters of Southern Africa: An Ecological Perspective.’ (Kluwer Academic Publishers: Dordrecht, Netherlands.)

Anderson, M. J. (2001). A new method for non-parametric multivariate analysis of variance. Austral Ecology 26, 32–46.

Anderson, M. J., Gorley, R. N., and Clarke, K. R. (2008). ‘PERMANOVA+ for PRIMER: Guide to Software and Statistical Methods.’(PRIMER-E, Plymouth Marine Laboratory: Plymouth, UK.)

Ashton, P. J., Roux, D. J., Breen, C. M., Day, J. A., Mitchell, S. A., Seaman, M. T., and Silberbauer, M. J. (2012). The freshwater science landscape in South Africa, 1900–2010. Water Research Commission Report number TT 530/12, Pretoria, South Africa.

Baldwin, D. S., Gigney, H., Wilson, J. S., Watson, G., and Boulding, A. (2008). Drivers of water quality in a large water storage reservoir during a period of extreme drawdown. Water Research 42, 4711–4724.
Drivers of water quality in a large water storage reservoir during a period of extreme drawdown.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtlCkt77J&md5=96ef382d89a48cb9a4f560c0b5fbd9b1CAS |

Bate, G. C., and Heelas, B. V. (1975). Studies on the nitrate nutrition of two indigenous Rhodesian grasses. Journal of Applied Ecology 12, 941–952.
Studies on the nitrate nutrition of two indigenous Rhodesian grasses.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE2sXhsFCmt7c%3D&md5=18ac79e0d4b07a999f9c933b9acf04ceCAS |

Bird, M. S., and Day, J. A. (2014). Wetlands in changed landscapes: the influence of habitat transformation on the physicochemistry of temporary depression wetlands. PLoS One 9, e88935.
Wetlands in changed landscapes: the influence of habitat transformation on the physicochemistry of temporary depression wetlands.Crossref | GoogleScholarGoogle Scholar |

Bird, M. S., Day, J. A., and Malan, H. L. (2014). The influence of biotope on invertebrate assemblages in lentic environments: a study of two perennial alkaline wetlands in the Western Cape, South Africa. Limnologica 48, 16–27.
The influence of biotope on invertebrate assemblages in lentic environments: a study of two perennial alkaline wetlands in the Western Cape, South Africa.Crossref | GoogleScholarGoogle Scholar |

Bump, J. K., Bergman, B. G., Schrank, A. J., Marcarelli, A. M., Kane, E. S., Risch, A. C., and Schütz, M. (2016). Nutrient release from moose bioturbation in aquatic ecosystems. Oikos , .
Nutrient release from moose bioturbation in aquatic ecosystems.Crossref | GoogleScholarGoogle Scholar |

Burnham, K. P., and Anderson, D. R. (2002). ‘Model Selection and Multimodel Inference: a Practical Information-Theoretic Approach.’ 2nd edn. (Springer-Verlag: New York, NY, USA.)

Burnham, K. P., and Anderson, D. R. (2004). Multimodel inference: understanding AIC and BIC in model selection. Sociological Methods & Research 33, 261–304.
Multimodel inference: understanding AIC and BIC in model selection.Crossref | GoogleScholarGoogle Scholar |

Carpenter, S. R., Caraco, N. F., Howarth, R. W., Sharpley, A. N., and Smith, V. H. (1998). Nonpoint pollution of surface waters with phosphorus and nitrogen. Ecological Applications 8, 559–568.
Nonpoint pollution of surface waters with phosphorus and nitrogen.Crossref | GoogleScholarGoogle Scholar |

Charley, J. L., and West, N. E. (1975). Plant-induced soil chemical patterns in some shrub-dominated semi-desert ecosystems of Utah. Journal of Ecology 63, 945–963.
Plant-induced soil chemical patterns in some shrub-dominated semi-desert ecosystems of Utah.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE28Xhs1ems7c%3D&md5=053a7266ae32d3a286faf88ed79e38ebCAS |

Clarke, K. R., and Gorley, R. N. P. (2006). ‘PRIMER v6: User Manual/Tutorial.’ (PRIMER-E, Plymouth Marine Laboratory: Plymouth, UK.)

Clarke, K. R., and Warwick, R. M. (2001). ‘Change in Marine Communities: an Approach to Statistical Analysis and Interpretation’, 2nd edn. (PRIMER-E, Plymouth Marine Laboratory: Plymouth, UK.)

Collen, B., Whitton, F., Dyer, E. E., Baillie, J. E. M., Cumberlidge, N., Darwall, W. R. T., Pollock, C., Richman, N. I., Soulsby, A., and Böhm, M. (2014). Global patterns of freshwater species diversity, threat and endemism. Global Ecology and Biogeography 23, 40–51.
Global patterns of freshwater species diversity, threat and endemism.Crossref | GoogleScholarGoogle Scholar |

Cook, P., Beck, V., Brereton, D., Clark, R., Fisher, B., Kentish, S., Toomey, J., and Williams, J. (2013). ‘Engineering Energy: Unconventional Gas Production.’ (Australian Council of Learned Academies: Melbourne, Vic., Australia)

Council of Canadian Academies (2014). Environmental impacts of shale gas extraction in Canada. Ottawa (ON): The expert panel on harnessing science and technology to understand the environmental impacts of shale gas extraction. Council of Canadian Academies, Ottawa, ON, Canada.

Cubasch, U., Wuebbles, D., Chen, D., Facchini, M. C., Frame, D., and Mahowald, N. W. J. G. (2013). Introduction. In ‘Climate Change 2013: The Physical Science Basis. Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change’. (Eds T. F. Stocker, D. P. Qin, G. K. M. Tignor, S. K. Allen, J. Boschung, A. Nauels, Y. Xia, V. Bex and P. M. Midgley.) pp. 119–158. (Cambridge University Press: Cambridge, UK.)

Darwall, W., Smith, K., Allen, D., Holland, R., Harrison, I., and Brooks, E. (2011). ‘The Diversity of Life in African Freshwaters: Underwater, Under Threat.’ (IUCN: Cambridge, UK.)

Davis, J. R., and Koop, K. (2006). Eutrophication in Australian rivers, reservoirs and estuaries – a southern hemisphere perspective on the science and its implications. Hydrobiologia 559, 23–76.
Eutrophication in Australian rivers, reservoirs and estuaries – a southern hemisphere perspective on the science and its implications.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xht1Wlu7o%3D&md5=a58e6aa917f9c6da37df2f47b18ceb4aCAS |

De Roeck, E. R., Vanschoenwinkel, B. J., Day, J. A., Xu, Y., Raitt, L., and Brendonck, L. (2007). Conservation status of large branchiopods in the Western Cape, South Africa. Wetlands 27, 162–173.
Conservation status of large branchiopods in the Western Cape, South Africa.Crossref | GoogleScholarGoogle Scholar |

De Roeck, E. R., Waterkeyn, A., and Brendonck, L. (2010). Life-history traits of Streptocephalus purcelli Sars, 1898 (Branchiopoda, Anostraca) from temporary waters with different phenology. Water S.A. 36, 323–328.

Dean, W. R. J., and Milton, S. J. (1999). Animal foraging and food. In ‘The Karoo. Ecological Patterns and Processes.’ (Eds W. R. J. Dean and S. J. Milton.) pp. 164–177. (Cambridge University Press: Cambridge, UK.)

Department of Water Affairs and Forestry (1996). ‘South African Water Quality Guidelines, Vol. 7: Aquatic Ecosystems’, 2nd edn. (DWAF: Pretoria, South Africa.)

Department of Water Affairs and Forestry (2002). ‘South African National Water Quality Monitoring Programmes Series. National Eutrophication Monitoring Programme.’ (DWAF, Pretoria, South Africa.)

Desmet, P. G., and Cowling, R. M. (1999). The climate of the Karoo – a functional approach. In ‘The Karoo. Ecological Patterns and Processes’. (Eds W. R. J. Dean and S. J. Milton.) pp. 3–16. (Cambridge University Press: Cambridge, UK.)

Dregne, H. E. (1976). ‘Soils of Arid Regions: Developments of Soil Science 6.’ (Elsevier Scientific Publishing: Amsterdam.)

Eaton, A. D., Clesceri, L. S., Rice, E. W., and Greenberg, A. E. (2005). ‘Standard Methods for the Examination of Water and Wastewater’, 21st edn. (American Public Health Association: Washington, DC, USA.)

Econometrix (2012). Karoo shale gas report. Special report on economic considerations surrounding potential shale gas resources in the southern Karoo of South Africa, Econometrix (Pty) Ltd, Johannesburg, South Africa.

Ferrar, K. J., Michanowicz, D. R., Christen, C. L., Mulcahy, N., Malone, S. L., and Sharma, R. K. (2013). Assessment of effluent contaminants from three facilities discharging Marcellus shale wastewater to surface waters in Pennsylvania, USA. Environmental Science & Technology 47, 3472–3481.
Assessment of effluent contaminants from three facilities discharging Marcellus shale wastewater to surface waters in Pennsylvania, USA.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXjsVWrur0%3D&md5=7433a0053453a6216353d3c8a09da6e5CAS |

Geel, C., Schulz, H.-M., Booth, P., and Horsfield, B. (2013). Shale gas characteristics of Permian black shales in South Africa: results from recent drilling in the Ecca Group (Eastern Cape). Energy Procedia 40, 256–265.
Shale gas characteristics of Permian black shales in South Africa: results from recent drilling in the Ecca Group (Eastern Cape).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhslCnt7nP&md5=68f867f120b516b0fd6d9328951d3073CAS |

Geldenhuys, J. N. (1982). Classification of the pans of the western Orange Free State according to vegetation structure, with reference to avifaunal communities. South African Journal of Wildlife Research 12, 55–62.

Grace, M. R., Hislop, T. M., Hart, B. T., and Beckett, R. (1997). Effects of saline groundwater on the aggregation and settling on suspended particles in a turbid Australian River. Colloids and Surfaces 120, 123–141.
Effects of saline groundwater on the aggregation and settling on suspended particles in a turbid Australian River.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXht1Khu7w%3D&md5=bffd588ad80cb7e35a1d17704ccbed70CAS |

Gregory, K. B., Vidic, R. D., and Dzombak, D. A. (2011). Water management challenges associated with the production of shale gas by hydraulic fracturing. Elements (Quebec) 7, 181–186.
Water management challenges associated with the production of shale gas by hydraulic fracturing.Crossref | GoogleScholarGoogle Scholar |

Hamer, M. L., and Brendonck, L. (1997). Distribution, diversity and conservation of Anostraca (Crustacea: Branchiopoda) in southern Africa. In ‘Studies on Large Branchiopod Biology and Conservation’. (Eds M. Simovich, C. Sassaman and D. Belk.) Vol. 125, pp. 1–12. (Springer Verlag: Berlin, Germany.)

Hart, D. R., Mulholland, P. J., Marzolf, E. R., Deangelis, D. L., and Hendricks, S. P. (1999). Relationships between hydraulic parameters in a small stream under varying low and seasonal conditions. Hydrological Processes 13, 1497–1510.
Relationships between hydraulic parameters in a small stream under varying low and seasonal conditions.Crossref | GoogleScholarGoogle Scholar |

Haughton, S. H. (1969). ‘Geological History of Southern Africa.’ (Geological Society of South Africa: Cape Town, South Africa.)

Hewitson, B. C., and Crane, R. G. (1996). Climate downscaling: techniques and application. Climate Research 7, 85–95.
Climate downscaling: techniques and application.Crossref | GoogleScholarGoogle Scholar |

Holm-Hansen, O., and Riemann, B. (1978). Chlorophyll-a determination: improvements in methodology. Oikos 30, 438–447.
Chlorophyll-a determination: improvements in methodology.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE1MXotlWgsg%3D%3D&md5=9dbc135270dc29a2da7086f7ccb12897CAS |

Hutchinson, G. E., Pickford, G. E., and Schuurman, J. F. M. (1932). A contribution to the hydrobiology of pans and other inland waters of South Africa. Archiv für Hydrobiologie 24, 1–154.

Kemp, P. H. (1963). Geological effects on surface waters in Natal. Nature 200, 1085–1085.
Geological effects on surface waters in Natal.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF2cXjslGrsg%3D%3D&md5=ca501cde6c57a9215d7823bcb0d50be5CAS |

Kirk, J. T. O. (1985). Effects of suspensoids (turbidity) on penetration of solar radiation in aquatic ecosystems. Hydrobiologia 125, 195–208.
Effects of suspensoids (turbidity) on penetration of solar radiation in aquatic ecosystems.Crossref | GoogleScholarGoogle Scholar |

Kristensen, P., Sondergaard, M., and Jeppesen, M. (1992). Resuspension in a shallow eutrophic lake. Hydrobiologia 228, 101–109.
Resuspension in a shallow eutrophic lake.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK38XhsFygsbw%3D&md5=0069dc7acaec0237ad489992bd24220cCAS |

Legendre, P., and Anderson, M. J. (1999). Distance-based redundancy analysis: testing multi-species responses in multi-factorial ecological experiments. Ecological Monographs 69, 1–24.
Distance-based redundancy analysis: testing multi-species responses in multi-factorial ecological experiments.Crossref | GoogleScholarGoogle Scholar |

Lemke, A. M., and Benke, A. C. (2009). Spatial and temporal patterns of microcrustacean assemblage structure and secondary production in a wetland ecosystem. Freshwater Biology 54, 1406–1426.
Spatial and temporal patterns of microcrustacean assemblage structure and secondary production in a wetland ecosystem.Crossref | GoogleScholarGoogle Scholar |

Lepš, J., and Šmilauer, P. (2003). ‘Multivariate Analysis of Ecological Data Using CANOCO.’ (Cambridge University Press: Cambridge, UK.)

Lewis, W. M. (2002). Causes for the high frequency of nitrogen limitation in tropical lakes. Verhandlungen des Internationalen Verein Limnologie 28, 210–213.

Macrae, M. L., English, M. C., Schiff, S. L., and Stone, M. (2007). Intra-annual variability in the contribution of tile drains to basin discharge and phosphorus export in a first-order agricultural catchment. Agricultural Water Management 92, 171–182.
Intra-annual variability in the contribution of tile drains to basin discharge and phosphorus export in a first-order agricultural catchment.Crossref | GoogleScholarGoogle Scholar |

Malan, H., and Day, J. (2012). Water quality and wetlands: defining ecological categories and links with land use. Report number 1921/1/12, Water Research Commission, Pretoria, South Africa.

Mason, S. J., Lindesay, J. A., and Tyson, P. D. (1994). Simulating drought in southern Africa using sea surface temperature variations. Water S.A. 20, 15–22.

Mauter, M. S., Alvarez, P. J., Burton, A., Cafaro, D. C., Chen, W., Gregory, K. B., Jiang, G., Li, Q., Pittock, J., Reible, D., and Schnoor, J. L. (2014). Regional variation in water-related impacts of shale gas development and implications for emerging international plays. Environmental Science & Technology 48, 8298–8306.
Regional variation in water-related impacts of shale gas development and implications for emerging international plays.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXltFGmtbw%3D&md5=b87511e0a7135a1581c0a37e8a3fece8CAS |

McAbendroth, L. P., Ramsay, M., Foggo, A., Rundle, S. D., and Bilton, D. T. (2005). Does macrophyte fractal complexity drive invertebrate diversity, biomass and body size distributions? Oikos 111, 279–290.
Does macrophyte fractal complexity drive invertebrate diversity, biomass and body size distributions?Crossref | GoogleScholarGoogle Scholar |

McArdle, B. H., and Anderson, M. J. (2001). Fitting multivariate models to community data: a comment on distance-based redundancy analysis. Ecology 82, 290–297.
Fitting multivariate models to community data: a comment on distance-based redundancy analysis.Crossref | GoogleScholarGoogle Scholar |

McBroom, M., Thomas, T., and Zhang, Y. L. (2012). Soil erosion and surface water quality impacts of natural gas development in east Texas, USA. Water 4, 944–958.
Soil erosion and surface water quality impacts of natural gas development in east Texas, USA.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhvVyqu7%2FK&md5=7548690edcaca200f8e8ac4d707ffee0CAS |

McBurnie, G., Davis, J., Thompson, R. M., Nano, C., and Brim-Box, J. (2015). The impacts of an invasive herbivore (Camelus dromedaries) on arid zone freshwater pools: an experimental investigation of the effects of dung on macroinvertebrate colonisation. Journal of Arid Environments 113, 69–76.
The impacts of an invasive herbivore (Camelus dromedaries) on arid zone freshwater pools: an experimental investigation of the effects of dung on macroinvertebrate colonisation.Crossref | GoogleScholarGoogle Scholar |

Meadows, M. E., and Watkeys, J. K. (1999). Paleoenvironments. In ‘The Karoo. Ecological Patterns and Processes’. (Eds W. R. J. Dean and S. J. Milton.) pp. 27–41. (Cambridge University Press: Cambridge, UK.)

Meintjes, S., Seaman, M. T., and Kok, D. J. (1994). Duration of inundation and change in physical and chemical characteristics of small temporary pans in South Africa. Hydrobiologia 281, 79–90.
Duration of inundation and change in physical and chemical characteristics of small temporary pans in South Africa.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2cXks1Cqsr8%3D&md5=cd85673f855bb7e96e7fe22641fa3878CAS |

Morse, D., Lawton, J., Dodson, M., and Williamson, M. (1985). Fractal dimension of vegetation and the distribution of arthropod body lengths. Nature 314, 731–733.
Fractal dimension of vegetation and the distribution of arthropod body lengths.Crossref | GoogleScholarGoogle Scholar |

Mucina, L., and Rutherford, M. C. E. (Eds) (2006). ‘The Vegetation of South Africa, Lesotho and Swaziland. Strelitzia 19.’ (South African National Biodiversity Institute: Pretoria, South Africa.)

Murray, K. E. (2013). State-scale perspective on water use and production associated with oil and gas operations, Oklahoma, US. Environmental Science & Technology 47, 4918–4925.
State-scale perspective on water use and production associated with oil and gas operations, Oklahoma, US.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXksF2htrk%3D&md5=9e1a4925849040e72e877f8ba384b438CAS |

Murray, R., Swana, K., Miller, J., Talma, S., Tredoux, G., Vengosh, A., and Darrah, T. (2015). The use of chemistry, isotopes and gases as indicators of deeper circulating groundwater in the main Karoo Basin. Report number 2254/1/15, Water Research Commission, Pretoria, South Africa.

Nielsen, D. L., Brock, M. A., Rees, G. N., and Baldwin, D. S. (2003). Effects of increasing salinity on freshwater ecosystems in Australia. Australian Journal of Botany 51, 655–665.
Effects of increasing salinity on freshwater ecosystems in Australia.Crossref | GoogleScholarGoogle Scholar |

Ollis, D., Snaddon, C. D., Job, N., and Mbona, N. (2013). Classification system for wetlands and other aquatic ecosystems in South Africa. User manual: inland systems. SANBI Biodiversity Series 22, South African Nation Biodiversity Institute, Pretoria, South Africa.

Ollis, D., Ewart-Smith, J., Day, J., Job, N., Macfarlane, D., Snaddon, C., Sieben, E., Dini, J., and Mbona, N. (2015). The development of a classification system for inland aquatic ecosystems in South Africa. Water S.A. 41, 727–745.
The development of a classification system for inland aquatic ecosystems in South Africa.Crossref | GoogleScholarGoogle Scholar |

Parsons, T. R., Maita, Y., and Lalli, C. M. (1984). ‘A Manual of Chemical and Biological Methods for Seawater Analysis.’ (Pergamon Press: New York, NY, USA.)

Pieterse, A. J. H., and Keulder, P. C. (Eds) (1982). ‘The Limnology of a Shallow, Turbid Impoundment, Wuras Dam: a Study of the Role of Suspensoids.’ Working Group for Limnology. (University of the Orange Free State: Bloemfontein, South Africa.)

Riato, L., Van Ginkel, C., and Taylor, J. C. (2014). Zooplankton and diatoms of temporary and permanent freshwater pans in the Mpumalanga Highveld region, South Africa. African Zoology 49, 113–127.
Zooplankton and diatoms of temporary and permanent freshwater pans in the Mpumalanga Highveld region, South Africa.Crossref | GoogleScholarGoogle Scholar |

Schulze, R. E. (1997). Climate. In ‘Vegetation of Southern Africa’. (Eds R. M. Cowling, D. M. Richardson and S. M. Pierce.) pp. 21–42. (Cambridge University Press: Cambridge, UK).

Schulze, R. E., Maharaj, M., Lynch, S. D., Howe, B. J., and Melvil-Thomson, M. (1997). South African atlas of agrohydrology and climatology. Water Research Commission Report number TT82/96, Pretoria, South Africa.

Senger, K., Buckley, S. J., Chevallier, L., Fagereng, Å., Galland, O., Kurz, T. H., Ogata, K., Planke, S., and Tveranger, J. (2015). Fracturing of doleritic intrusions and associated contact zones: implications for fluid flow in volcanic basins. Journal of African Earth Sciences 102, 70–85.
Fracturing of doleritic intrusions and associated contact zones: implications for fluid flow in volcanic basins.Crossref | GoogleScholarGoogle Scholar |

Silberbauer, M. J., and King, J. M. (1991). Geographical trends in the water chemistry of wetlands in the south-western Cape province, South Africa. Southern African Journal of Aquatic Sciences 17, 82–88.
Geographical trends in the water chemistry of wetlands in the south-western Cape province, South Africa.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK38XlsFOitbw%3D&md5=b84ffb52418afabb083eb79a6acf08d5CAS |

Steyl, G., van Tonder, G. J., and Chevallier, L. (2012). State of the art: fracking for shale gas exploration in South Africa and the impact on water resources. Water Research Commission, Report number KV 294/11, Pretoria, South Africa.

ter Braak, C. J. F., and Šmilauer, P. (2002). ‘CANOCO Reference Manual and CanoDraw for Windows User's Guide: Software for Canonical Community Ordination (version 4.5).’ (Microcomputer Power: Ithaca, NY, USA.)

Tucker, A. R., and van Tonder, G. (2015). The Karoo fracking debate: a Christian contribution to the world communities of faith. Science and Engineering Ethics 21, 631–653.
The Karoo fracking debate: a Christian contribution to the world communities of faith.Crossref | GoogleScholarGoogle Scholar |

US Environmental Protection Agency (2011). ‘Plan to Study the Potential Impacts of Hydraulic Fracturing on Drinking Water Resources.’ (EPA, Office of Research and Development: Washington, DC, USA.)

Uys, A. C. (1997). The invertebrates of temporary rivers in the Eastern Cape, South Africa. Ph.D. Thesis, Rhodes University, Grahamstown, South Africa.

Van Ginkel, C. E., Hohls, B. C., Belcher, A., Vermaak, E., and Gerber, A. (2001). Assessment of the trophic status project. Internal Report number N/0000/00/DEQ/1799, Institute for Water Quality Studies, Department of Water Affairs and Forestry, Pretoria, South Africa.

van Tonder, G. J., de Lange, F., Steyl, G., and Vermeulen, D. (2013). Potential impacts of fracking on groundwater in the Karoo Basin of South Africa. 13th Biennial Conference of the Groundwater Division of the Geological Society of South Africa’, 17–18 September 2013, Durban, South Africa. pp. 1–10. (Institute for Groundwater Studies: Bloemfontein, South Africa.)

Vengosh, A., Jackson, R. B., Warner, N., Darrah, T. H., and Kondash, A. (2014). A critical review of the risks to water resources from unconventional shale gas development and hydraulic fracturing in the United States. Environmental Science & Technology 48, 8334–8348.
A critical review of the risks to water resources from unconventional shale gas development and hydraulic fracturing in the United States.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXjslCru7o%3D&md5=f4086c39f0e2edc5f9e6d350f263ca81CAS |

Vermuelen, P. D. (2012). A South African perspective on shale gas hydraulic fracturing. In ‘International Mine Water Association Annual Conference’, 30 September–4 October 2012, Bunbury, WA, Australia. (Eds C. D. McCullough, M. A. Lund, and L. Wyse.) pp. 149–156. (Institute for Groundwater Studies: Bloemfontein, South Africa.)

Walmsley, R. D. (1984). A chlorophyll-a trophic status classification for South African impoundments. Journal of Environmental Quality 13, 97–104.
A chlorophyll-a trophic status classification for South African impoundments.Crossref | GoogleScholarGoogle Scholar |

Walmsley, R. D., and Bruwer, C. A. (1980). Water transparency characteristics of South African impoundments. Journal of the Limnological Society of Southern Africa 6, 69–76.
Water transparency characteristics of South African impoundments.Crossref | GoogleScholarGoogle Scholar |

Walmsley, R. D., Butty, M., van der Piepen, H., and Grobler, D. (1980). Light penetration and the interelationships between optical parameters in a turbid subtropical impoundment. Hydrobiologia 70, 145–157.
Light penetration and the interelationships between optical parameters in a turbid subtropical impoundment.Crossref | GoogleScholarGoogle Scholar |

Warner, N. R., Christie, C. A., Jackson, R. B., and Vengosh, A. (2013). Impacts of shale gas wastewater disposal on water quality in western Pennsylvania. Environmental Science & Technology 47, 11849–11857.
Impacts of shale gas wastewater disposal on water quality in western Pennsylvania.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhsFCjt7nF&md5=464465bd0068fc2b0c1dfa0228fba183CAS |

Watkeys, M. K. (1999). Soils of the arid south-western zone of Africa. In ‘The Karoo. Ecological Patterns and Processes’. (Eds W. R. J. Dean and S. J. Milton.) pp. 17–26. (Cambridge University Press: Cambridge, UK.)

Welschmeyer, N. A. (1994). Fluorometric analysis of chlorophyll-a in the presence of chlorophyll-b and pheopigments. Limnology and Oceanography 39, 1985–1992.
Fluorometric analysis of chlorophyll-a in the presence of chlorophyll-b and pheopigments.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXks1Sru70%3D&md5=227a81557baf112c4fbb6e7a5f0e69daCAS |

Wetzel, R. G. (2001). ‘Limnology: Lake and River Ecosystems.’ (Academic Press: San Diego, CA, USA.)

Williams, D. D. (2006). ‘The Biology of Temporary Waters.’ (Oxford University Press: New York, NY, USA.)

Williams, P., Whitfield, M., Biggs, J., Bray, S., Fox, G., Nicolet, P., and Sear, D. (2004). Comparative biodiversity of rivers, streams, ditches and ponds in an agricultural landscape in Southern England. Biological Conservation 115, 329–341.
Comparative biodiversity of rivers, streams, ditches and ponds in an agricultural landscape in Southern England.Crossref | GoogleScholarGoogle Scholar |

Williams, P., Whitfield, M., and Biggs, J. (2008). How can we make new ponds biodiverse? A case study monitored over 7 years. Hydrobiologia 597, 137–148.
How can we make new ponds biodiverse? A case study monitored over 7 years.Crossref | GoogleScholarGoogle Scholar |

Winter, J. G., Dillon, P. J., Futter, M. N., Nicholls, K. H., Scheider, W. A., and Scott, L. D. (2002). Total phosphorus budgets and nitrogen loads: Lake Simcoe, Ontario (1990 to 1998). Journal of Great Lakes Research 28, 301–314.
Total phosphorus budgets and nitrogen loads: Lake Simcoe, Ontario (1990 to 1998).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XovVWkur4%3D&md5=276d393ef7d4991fc448e00cc48a0a43CAS |

Zahran, H. H. (1999). Rhizobium–legume symbiosis and nitrogen fixation under severe conditions and in an arid climate. Microbiology and Molecular Biology Reviews 63, 968–989.
| 1:CAS:528:DC%2BD3cXjtVShsQ%3D%3D&md5=333b509e4249bbd6e079cee63f3ab06aCAS |