Pseudomonas putida inoculation promotes submerged plant Vallisneria natans growth by carbon conversion in a plant–microbe interaction
Lin Gan A , Hui Zhao B , Aili Wang A , Sanshan Li A , Jia Liu C and Liuyan Yang A D
+ Author Affiliations
- Author Affiliations
A State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Xianlin Road 163, Nanjing, 210023, P.R. China.
B Nanjing University Research Institute (Changshu) Co. Ltd, Huanhunan Road 1, Changshu, 215500, P.R. China.
C State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Siping Road 1239, Shanghai, 200092, P.R. China.
D Corresponding author. Email: yangly@nju.edu.cn
Marine and Freshwater Research 69(5) 851-858 https://doi.org/10.1071/MF17117
Submitted: 29 April 2017 Accepted: 12 January 2018 Published: 16 April 2018
Abstract
Submerged plant growth is limited by the reduction of underwater photosynthesis attributed to low CO2 availability, as well as light limitation associated with underwater conditions. Heterotrophic bacteria and fungi play an important role in local aqueous dissolved inorganic carbon (DIC) content surrounding submerged plants. In order to investigate the effects of carbon conversion in plant–microbe interactions on plant growth, in the present study we inoculated the plant medium of Vallisneria natans with Pseudomonas putida KT2440 and measured carbon conversion in the system, as well as several indices of plant growth. The quantity of P. putida KT2440 increased twofold because of the availability of organic matter produced by V. natans. Similarly, P. putida KT2440 supplied DIC for V. natans, improving its photosynthetic rate. Moreover, the significantly higher leaf area, specific leaf area and fresh biomass of V. natans attributed to the presence of P. putida KT2440 demonstrated that the interaction between V. natans and P. putida enhanced the efficiency of nutrient and CO2 uptake by V. natans, promoting V. natans growth. Therefore, we suggest that the carbon and oxygen microcycle based on the protocooperation of V. natans and P. putida KT2440 may accelerate the transformation of carbon to increase carbon availability to promote the growth of both plant and microbe.
Additional keywords: chlorophyll content, pH, root : shoot ratio.
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