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RESEARCH ARTICLE
Contents Vol 59(10)

Changes in sediment microbial community structure within a large water-storage reservoir during an extreme drawdown event

A. N. Boulding A B , G. N. Rees B C , D. S. Baldwin B , P. J. Suter A and G. O. Watson B
+ Author Affiliations
- Author Affiliations

A Department of Environmental Management and Ecology, La Trobe University, University Drive, Wodonga, Vic. 3690, Australia.

B The Murray-Darling Freshwater Research Centre, PO Box 991, Wodonga, Vic. 3690, Australia.

C Corresponding author. Email: Gavin.Rees@csiro.au

Marine and Freshwater Research 59(10) 890-896 https://doi.org/10.1071/MF07232
Submitted: 3 December 2007  Accepted: 25 July 2008   Published: 27 October 2008

Abstract

Although drought and drying of waters occur globally, the effect of drying on sediment microbial communities underpinning aquatic biogeochemical processes is poorly understood. We used the molecular method of terminal-restriction fragment length polymorphism (T-RFLP) to assess changes in the microbial community structure of sediments undergoing different levels of inundation and drying within a reservoir during drawdown in a drought. Sediments with three hydrological conditions were investigated: dry sediments (no overlying water), littoral sediments (covered with 1–2 mm water) and inundated sediments (covered with >1 m water). Sampling was done in winter 2006 (August) and summer 2007 (January) in Lake Hume, Australia. The microbial communities differed significantly between the different levels of inundation at each sampling time. Community structure also changed significantly within each site between winter 2006 and summer 2007, possibly influenced by the change of season or protracted drying. Sites that were ‘littoral’ in winter 2006 became ‘dry’ in summer 2007, and became more similar to communities that were ‘dry’ at both sampling times. This suggested that the hydrological history of specific sites did not heavily influence the response of microbial communities to severe drying, and all communities undergoing ‘dry’ conditions within the summer 2007 sampling responded similarly.

Additional keywords: drought, drying, sediments, terminal-restriction fragment length polymorphism.


Acknowledgements

This work was funded by La Trobe University and the Terry Hillman Honours Educational Prize (awarded to A. N. Boulding). We thank Dr W. Paul for statistical advice and M. Ziino at the Australian Genome Research Facility/Walter and Eliza Hall Institute for help with the T-RFLP analysis. We thank Professor M. Koschorreck and an anonymous referee for improving the manuscript.


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