Immunosuppression in Sydney rock oysters (Saccostrea glomerata) and QX disease in the Hawkesbury River, Sydney
Daniel Butt A and David Raftos A BA Department of Biological Sciences, Macquarie University, North Ryde, NSW 2109, Australia.
B Corresponding author. Email: draftos@bio.mq.edu.au
Marine and Freshwater Research 58(2) 213-221 https://doi.org/10.1071/MF06080
Submitted: 10 May 2006 Accepted: 30 October 2006 Published: 16 February 2007
Abstract
This study assessed links between host fitness, environmental change and opportunistic parasite infections in a dynamic estuary system. The Hawkesbury River in New South Wales is the most recent Sydney rock oyster growing area to experience outbreaks of infectious QX disease. This area was used to examine a relationship between the intensity of QX disease and inhibition of the oyster immune system. Oysters were grown at various sites along the river and periodically monitored for general condition, total haemolymph protein content, antibacterial capacity and phenoloxidase activity. Phenoloxidase activity was significantly inhibited during a key period of Marteilia sydneyi infectivity in late summer 2005. The degree to which phenoloxidase was inhibited strongly correlated with the intensity of M. sydneyi infection. The data suggest that the presence of some transient environmental stressor may have affected phenoloxidase activity during a key period of infection and increased the susceptibility of oysters to disease. These results provide further evidence for a specific relationship between decreased phenoloxidase activity and susceptibility to QX infection.
Additional keywords: environmental stress, oysters, phenoloxidase, QX disease.
Acknowledgements
Our thanks go to the oyster farmers of the Hawkesbury River for their support and access to field sites, particularly Robbie Moxham and John Stubbs. Thanks also to Rob Adlard of the Queensland Museum for QX-infection information, and DPI Fisheries for field support. We thank the Hornsby Council and Sydney Water for providing water quality data. This study was funded in part by an Australian Research Council Linkage grant (LP 0453461) in conjunction with the NSW DPI.
Adlard, R. D. , and Ernst, I. (1995). Extended range of the oyster pathogen, Marteilia sydneyi. Bulletin of the European Association of Fish Pathologists 15, 119–121.
Fisher, W. S. , Olivera, L. M. , Walkera, W. W. , Manning, C. S. , and Lytleb, T. F. (1999). Decreased resistance of eastern oyster (Crassostrea virginica) to a protozoan pathogen (Perkinsus marinus) after sub-lethal exposure to tributyltin oxide. Marine Environmental Research 47, 185–201.
| Crossref | GoogleScholarGoogle Scholar |
Hellio, C. , Bado-Nilles, A. , Gagnaire, B. , Renault, T. , and Thomas-Guyon, H. (2007). Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific-oyster, Crassostrea gigas (Thunberg) in vitro. Fish & Shellfish Immunology 22, 433–440.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Kleeman, S. N. , and Adlard, R. D. (2000). Molecular detection of Marteilia sydneyi, pathogen of Sydney rock oysters. Diseases of Aquatic Organisms 40, 137–146.
| PubMed |
Lacoste, A. , Malhamb, S. K. , Gelebarta, F. , Cueffa, A. , and Pouleta, S. A. (2002). Stress-induced immune changes in the oyster Crassostrea gigas. Developmental and Comparative Immunology 26, 1–9.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Ordas, M. C. , Ordas, A. , Beloso, C. , and Figueras, A. (2000). Immune parameters in carpet shell clams naturally infected with Perkinsus atlanticus. Fish & Shellfish Immunology 10, 597–609.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Sorrentino, R. P. , Carton, Y. , and Govind, S. (2002). Cellular immune response to parasite infection in the Drosophila lymph gland is developmentally regulated. Developmental Biology 243, 65–80.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Volety, A. K. , Oliver, L. M. , Genthner, F. J. , and Fisher, W. S. (1999). A rapid tetrazolium dye reduction assay to assess the bacteriacidal activity of oyster (Crassostrea virginica) hemocytes against Vibrio parahaemolyticus. Aquaculture 172, 205–222.
| Crossref | GoogleScholarGoogle Scholar |
![](../../media/share/OpenURL_Image.gif)
Wang, L. U. , and Chen, J. C. (2005). The immune response of white shrimp Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus at different salinity levels. Fish & Shellfish Immunology 18, 269–278.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
![](../../media/share/OpenURL_Image.gif)
Wang, F. I. , and Chen, J. C. (2006). Effect of salinity on the immune response of tiger shrimp Penaeus monodon and its susceptibility to Photobacterium damselae subsp. damselae. Fish & Shellfish Immunology 20, 671–681.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
![](../../media/share/OpenURL_Image.gif)
Wesche, S. J. (1995). Outbreaks of Marteilia sydneyi in Sydney rock oysters and their relationship with environmental pH. Buletin of the European Association of Fish Pathologists 15, 23–27.
![](../../media/share/OpenURL_Image.gif)
Wesche, S. J. , Adlard, R. D. , and Lester, R. J. G. (1999). Survival of spores of the oyster pathogen Marteilia sydneyi (Protozoa, paramyxea) as assessed using fluorogenic dyes. Diseases of Aquatic Organisms 36, 221–226.
![](../../media/share/OpenURL_Image.gif)