
%0 Journal Article
%A Hyne, RV 
%A Padovan, A 
%A Parry, DL 
%A Renaud, SM 
%T Increased fecundity of the cladoceran <I>Moinodaphnia macleayi</I> on a diet supplemented with a green alga, and its use in uranium toxicity tests
%J Marine and Freshwater Research
%D 1993/06/01
%V 44
%N 3
%P 389-399
%K microalgae, fatty acid, nutrition
%R https://doi.org/10.1071/MF9930389
%U https://www.publish.csiro.au/paper/MF9930389 
%X The effects of two synthetic diets and four algal diets on the survival and fecundity of Moinodaphnia macleayi for use in life-cycle toxicity tests were determined. The synthetic foods were a suspension of blended cichlid fish pellets with powdered alfalfa, either prepared fresh or fermented in natural water for 3 days. The algae, isolated from the habitat of M. rnacleayi in the Alligator Rivers Region, were Selenastrum sp., Ankistrodesmus (falcatus?), Scenedesmus quadricauda and a Chlamydomonas sp. Each alga was tested alone or as a supplement to the fermented synthetic food. The diet that supported the greatest number of young and the highest survival rate over a 5-day period was a combination of the fermented synthetic food and Chlamydomonas sp. Analysis of the fatty acid composition of the lipid fraction of the four algal species showed that Chlamydomonas sp., Selenastrum sp. and Scenedesmus quadricauda had significant concentrations of the highly unsaturated fatty acids 18 : 2(n-6), 18 : 3(n-6) and 18: 3(n-3). In terms of size and dry weight, Chlamydomonas sp. was the smallest of the algal cells studied. The toxicity of uranium to M. macleayi survival, determined under standard conditions, was a lowest-observed-effect concentration (LOEC) of 200 µg L-1 after 48 h and 25 µg L-1 after 5 days. 
