
%0 Journal Article
%A Innes, B. H. 
%A Grewe, P. M. 
%A Ward, R. D. 
%T PCR-based genetic identification of marlin and other billfish
%J Marine and Freshwater Research
%D 1998/10/19
%V 49
%N 5
%P 383-388
%K 
%R https://doi.org/10.1071/MF98007
%U https://www.publish.csiro.au/paper/MF98007 
%X A genetic test was developed for the identification of the six species of billfish found in Australian waters (black marlin, Indo&#8211;Pacific blue marlin, striped marlin, Indo&#8211;Pacific sailfish, shortbill spearfish and broadbill swordfish). The test was based on the PCR&#8211;RFLP analysis of a 1400 bp region of the mitochondrial DNA molecule, the d-loop, using four restriction enzymes (Hinf I, Rsa I and Sau3A I andTaq I). A total of 33 composite haplotypes were observed among 160 fish; all were species-specific. Three of the species&#8212;black marlin, striped marlin and broadbill swordfish&#8212;showed sufficient intra-specific variation to be useful in population structure analyses. 
