
%0 Journal Article
%A Gibbs, CF 
%T Chlorophyll b Interference in the Fluorometric Determination of Chlorophyll a and 'Phaeo-Pigments'
%J Marine and Freshwater Research
%D 1979/10/01
%V 30
%N 5
%P 597-606
%K 
%R https://doi.org/10.1071/MF9790597
%U https://www.publish.csiro.au/paper/MF9790597 
%X A quantitative estimate was made of the interference by chlorophyll b in the fluorometric determination of chlorophyll a using an Aminco-Bowman spectrofluorometer. The undegraded (or 'living') chlorophyll a concentration was underestimated by an amount equal to the concentration of chlorophyll b present. The value for chlorophyll a uncorrected for phaeophytin was increased by 30% of the chlorophyll b concentration. The acetone to water ratio was found to affect the acidification factor only at aqueous acetone concentrations above 90%. The need for standardization of solvent and wavelengths is discussed, and the implications of chlorophyll b occurring in marine samples are considered. 
