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Functional Plant Biology

Volume 52 Number 2 2025

FP24255Advances in CRISPR/Cas9 technology: shaping the future of photosynthetic microorganisms for biofuel production

Samreen Arshad, Muhammad Luqman Qadir, Nazim Hussain, Qurban Ali

, Shiming Han and Daoud Ali

CRISPR technology have been used to increase biomass and lipid content in microalgae, which has benefits for industrial application in the production of biofuels. This review highlights the attempts made so far to target microalgae genome modification, discusses the prospects for developing the CRISPR platform for large-scale genome modification of microalgae, identifies the opportunities and challenges related to the development anddistribution of CRISPR/Cas9 components, and how different CRISPR approaches can be used in the modification of algae and cyanobacteria.

FP24255 Abstract | FP24255 Full Text | FP24255PDF (2.6 MB) Open Access Article

FP24216The relationship between GABA content and desiccation tolerance at five developmental stages of wheat (Triticum durum) seeds

Nezar H. Samarah

, Nisreen A. AL-Quraan

and Roa’a I. Shawah’en

Drying wheat (Triticum durum) seeds attached to their spikes improved seed germination (desiccation tolerance), mean germination time, and seedling length at the milk stage of seed development compared with seed dried detached from spikes. The improvement of seed germination of spiked-dried seeds was associated with high accumulation of GABA metabolites, lower MDA content, higher expression of dehydrin genes (dhn, wcor, and dreb).