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Plant function and evolutionary biology
RESEARCH ARTICLE

Leaf manganese and phenolics as proxies of soil acidification and phosphorus acquisition mechanisms in lentil cultivars on alkaline soil

Georgia S. Theologidou A , Ioannis Ipsilantis B and Ioannis T. Tsialtas https://orcid.org/0000-0002-2837-136X A *
+ Author Affiliations
- Author Affiliations

A Aristotle University of Thessaloniki, Faculty of Agriculture, Laboratory of Agronomy, Thessaloniki 541 24, Greece.

B Aristotle University of Thessaloniki, Faculty of Agriculture, Laboratory of Soil Science, Thessaloniki 541 24, Greece.

* Correspondence to: tsialtas01@windowslive.com

Handling Editor: Sergey Shabala

Functional Plant Biology 50(12) 1028-1036 https://doi.org/10.1071/FP23109
Submitted: 4 January 2023  Accepted: 14 September 2023  Published: 9 October 2023

© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing

Abstract

Leaf manganese (Mn) concentration has been used as a proxy for root exudation and phosphorus (P) uptake under controlled conditions, but there are limited field studies that confirm its validity. On an alkaline, P-poor soil, four lentil cultivars (‘Samos’, ‘Thessaly’, ‘Flip’, ‘Algeria’) received two P rates (0 and 26.2 kg P ha−1), for two growing seasons, to study whether aboveground assessments [leaf P, Mn, phenolic concentration (TPhe)] can approximate rhizosphere physiological traits related to P acquisition [soil acidification (ΔpH), arbuscular mycorrhizal fungi (AMF) colonisation, acid phosphatase activity (APase)]. Phosphorus addition had no effect on the determined traits. Cultivars differed in leaf P, Mn, TPhe and AMF, but there was no clear pattern relating aboveground traits to rhizosphere traits related to P acquisition, thus not confirming that leaf Mn can be a proxy of root exudation. Of three growth stages [V 7–8, R1 (first bloom), R4 (flat pod)], R1 seemed to be critical, showing the highest leaf P, ΔpH, AMF and TPhe. Precipitation and temperatures over the growing season were determinants of lentil responses affecting rhizosphere activity, soil P availability and finally leaf traits. In conclusion, in lentil on alkaline and P-limiting soils, high leaf Mn and phenolic concentration are not reliable indicators of rhizosphere P-acquiring mechanisms.

Keywords: acid phosphatase activity, alkalinity, carboxylates, exudation, grain legumes, Mediterranean soils, mycorrhiza, soil pH.

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