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Plant function and evolutionary biology
RESEARCH ARTICLE

Combinatorial transcriptomics and metabolomics analysis reveals the effects of the harvesting stages on the accumulation of phenylpropanoid metabolites in Lonicera japonica

Zhifang Ran A B # , Weina Ding A # , Hongxia Yu C , Li Zhang D , Lei Fang https://orcid.org/0000-0003-3999-7170 A * , Lanping Guo https://orcid.org/0000-0001-6974-1411 E * and Jie Zhou https://orcid.org/0000-0002-1241-2876 A *
+ Author Affiliations
- Author Affiliations

A School of Biological Science and Technology, University of Jinan, Jinan 250022, China.

B School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250355, PR China.

C Weihai (Wendeng) Authentic Ginseng Industry Development Co. Ltd., Wendeng 264407, China.

D Shandong Zhongping Pharmaceutical Industry, Linyi 273399, China.

E State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.

# These authors contributed equally to this paper

Handling Editor: David Cahill

Functional Plant Biology 50(10) 808-820 https://doi.org/10.1071/FP23033
Submitted: 12 February 2023  Accepted: 8 August 2023   Published: 23 August 2023

© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing

Abstract

The flower buds of Lonicera japonica are widely used for its high medicinal value. It is reported that the accumulation of phenylpropanoids in the buds of L. japonica is affected by the stage at which it is harvested. However, the changes of active components and the underlying mechanisms in flower buds at different harvesting stages have not been reported. Integrative analyses of transcriptomics and metabolomics was used to explore the underlying mechanism of harvesting stages (green bud, GB; and white bud, WB) on the phenylpropanoids metabolites accumulation in L. japonica. The result showed that 3735 differentially expressed genes were identified, and the genes related to glycolysis/gluconeogenesis and phenylalanine biosynthesis pathway were significantly upregulated in GB stage. A total of 510 differential metabolites were identified in GB stage. Among them, 14 phenylpropanoids were changed during the GB and WB, seven of which increased in GB, including caffeic acid, sauchinone, coniferin, secoisolariciresinol diglucoside, scopolin, methyl cinnamate, chlorogenic acid, 7-hydroxycoumarin, while others such as sibiricose A6, coumarin, eleutheroside E decreased. Further correlation analysis showed that the unigenes for CSE, CAD, bg1, ADH, ALDH, DLAT and ENO significantly correlated with the 10 phenylpropanoid. The above results would provide basic data for the selection of harvesting stages in the production of L. japonica.

Keywords: correlation analysis, differentially expressed genes, harvesting stage, Lonicera japonica Thunb., metabolomics, phenylpropanoid metabolites, transcription factors, transcriptomics.

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