Biochemical and molecular approach of oxidative damage triggered by water stress and rewatering in sunflower seedlings of two inbred lines with different ability to tolerate water stress
Federico Ramírez A , Maximiliano Escalante A , Ana Vigliocco B , M. Verónica Pérez-Chaca C , Mariana Reginato B , Alicia Molina C , Julio A. Di Rienzo D , Andrea Andrade B and Sergio Alemano
B E
+ Author Affiliations
- Author Affiliations
A Laboratorio de Fisiología Vegetal, Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto (UNRC), 5800-Río Cuarto, Córdoba, Argentina.
B Laboratorio de Fisiología Vegetal, Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, UNRC, Instituto de Investigaciones Agrobiotecnológicas-Consejo Nacional de Investigaciones Científicas y Técnicas (INIAB-CONICET), 5800-Río Cuarto, Córdoba, Argentina.
C Departamento de Bioquímica y Ciencias Biológicas, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, 5700-San Luis, Argentina.
D Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba, 5000-Córdoba, Argentina.
E Corresponding author. Email: salemano@exa.unrc.edu.ar
Functional Plant Biology 47(8) 727-743 https://doi.org/10.1071/FP19264
Submitted: 10 September 2019 Accepted: 10 March 2020 Published: 1 June 2020
Abstract
Water stress accelerates the generation of reactive oxygen species, which trigger a cascade of antioxidative defence mechanisms comprising enzymatic and nonenzymatic antioxidants. The aim of this study was to investigate the oxidative damage and the antioxidative defence systems in seedlings of the water stress-tolerant (B71) and the sensitive (B59) inbred lines of sunflower (Helianthus annuus L.) in response to water stress and rewatering. In addition, we characterised the transcriptomic profile associated with enzymatic antioxidative defence. An elevated electrolyte leakage in B59 indicated increased plasmatic membrane permeability, which correlated with greater sensitivity to water stress. In response to water stress, both lines showed an increase in malondialdehyde and H2O2 content but these increases were more noticeable in the sensitive line. In both lines, an increase in enzymatic activity (e.g. peroxidase and ascorbate peroxidase) was not sufficient to overcome the H2O2 accumulation triggered by water stress. Upon water stress, the overall expression level of genes associated with the enzymatic antioxidant system increased in B71 and decreased in B59, which showed downregulated levels of most genes in the shoots. The general profile of phenolic compounds was clearly different between organs and between inbred lines. The B59 line activated nonenzymatic antioxidant responses to counteract the oxidative stress caused by water stress. The tolerance of B71 to water stress could be associated with compensatory antioxidant mechanisms based on the expression of genes encoding enzyme components of the ascorbate–glutathione and redoxin cycles, which contributed to explaining, at least partly, the response of this line.
Additional keywords: antioxidative response, gene expression, Helianthus annuus L., phenolic compounds, reactive oxygen species.
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