Induction of the heat shock response in Arabidopsis by heat shock protein 70 inhibitor VER-155008
Erina Matsuoka A , Naoki Kato B C and Masakazu Hara
A B C D
+ Author Affiliations
- Author Affiliations
A Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Shizuoka, Shizuoka 422-8529, Japan.
B Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Shizuoka, Shizuoka 422-8529, Japan.
C R & D Center, Menicon Co., Ltd, 5-1-10 Takamoridai, Kasugai, Aichi 487-0032, Japan.
D Corresponding author. Email: hara.masakazu@shizuoka.ac.jp
Functional Plant Biology 46(10) 925-932 https://doi.org/10.1071/FP18259
Submitted: 16 October 2018 Accepted: 1 June 2019 Published: 20 June 2019
Abstract
The heat shock protein 90 (HSP90) inhibitor, geldanamycin, is a chemical inducer of the heat shock response (HSR) in Arabidopsis. Geldanamycin is thought to activate the heat shock signal by dissociating the HSP90-heat shock factor (HSF) complex. Recent studies have indicated that plant HSP70 is also associated with HSF, suggesting that inhibition of HSP70 may induce the HSR. However, no studies have been conducted to test this hypothesis. Here, we found that a specific HSP70 inhibitor VER-155008 activated the promoter of a small HSP gene (At1 g53540, HSP17.6C-CI) of Arabidopsis, which was shown to be activated by geldanamycin and other HSP90 inhibitors. The production of HSP17.6C-CI, HSP70 and HSP90.1 proteins in Arabidopsis was enhanced by the addition of VER-155008. The reduction of chlorophyll contents by heat shock was ameliorated by VER-155008. Chaperone analyses indicated that VER-155008 inhibited the chaperone activities of wheat germ extract and human HSP70/HSP40, respectively. These results suggest that the inhibition of HSP70 by VER-155008 enhanced the heat tolerance of Arabidopsis by inducing the HSR in the plant.
Additional keywords: Arabidopsis thaliana, geldanamycin, heat shock protein 70 inhibitors, heat shock response, sanguinarine, VER-155008.
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