The rate of drying determines the extent of desiccation tolerance in Physcomitrella patens
Joshua L. Greenwood A B and Lloyd R. Stark A
+ Author Affiliations
- Author Affiliations
A School of Life Sciences, University of Nevada, 4505 Maryland Parkway, Box 454004, Las Vegas, NV 89154-4004, USA.
B Corresponding author. Email: greenw33@unlv.nevada.edu
Functional Plant Biology 41(5) 460-467 https://doi.org/10.1071/FP13257
Submitted: 28 August 2013 Accepted: 16 November 2013 Published: 2 January 2014
Abstract
The effect of differential drying rates on desiccation tolerance in Physcomitrella patens (Hedw.) Bruch & Schimp. is examined. In order to provide more evidence as to the status of desiccation tolerance in P. patens, a system was designed that allowed alteration of the rate of water loss within a specific relative humidity. An artificial substrate consisting of layers of wetted filter paper was used to slow the drying process to as long as 284 h, a significant increase over the commonly used method of exposure (saturated salt solution). By slowing the rate of drying, survival rates and chlorophyll fluorescence parameters improved, and tissue regeneration time was faster. These results indicate a trend where the capacity for desiccation tolerance increases with slower drying, and reveal a much stronger capacity for desiccation tolerance in P. patens than was previously known.
Additional keywords: bryophyte, chlorophyll fluorescence parameters, moss, tissue regeneration, water loss.
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