Tomato mitogen activated protein kinases regulate the expression of extracellular invertase Lin6 in response to stress related stimuli
Tae Kyung Hyun A , Anja Hoffmann A , Alok K. Sinha B C and Thomas Roitsch AA Julius-von-Sachs-Institut fuer Biowissenschaften, Universitaet Wuerzburg, Julius-von-Sachs-Platz 2, 97082 Wuerzburg, Germany.
B National Institute of Plant Genome Research, PB No. 10531, Aruna Asaf Ali Road, New Delhi 110067, India.
C Corresponding author. Email: alok_sinha@nipgr.res.in
Functional Plant Biology 36(12) 1088-1097 https://doi.org/10.1071/FP09136
Submitted: 4 June 2009 Accepted: 31 August 2009 Published: 3 December 2009
Abstract
Activation of mitogen-activated protein kinases (MAPKs) is a common reaction of plant cells in defence-related signal transduction pathways. Since the downstream events after the activation of MAPKs are largely unknown in plants, the role of MAPKs in the coordinate regulation of defence reactions and primary carbon metabolism by stress related stimuli has been analysed in tomato (Lycopersicon peruvianum Mill.). Thus, the relationship between MAPK, LpMPK2 and LpMPK3 and extracellular invertase Lin6, as the key enzyme of an apoplasmic phloem unloading pathway, has been analysed. It was observed that the mRNAs of LpMPK3 and Lin6 are sequentially induced by the same set of stress related stimuli, wounding, a fungal elicitor derived from Fusarium oxysporum lycopersici, the endogenous plant derived elicitor PGA and salt stress, while LpMPK2 transcripts are constitutively expressed. In a gain of function approach, a His-tagged version of LpMPK2 and a HA-tagged version of LpMPK3 were transiently and functionally expressed in leaves of transgenic tobacco (Nicotiana tabacum L.) plants expressing the β-glucuronidase reporter gene under control of the Lin6 promoter via agro-infection. The induction of the Lin6 promoter, as revealed by an increase in β-glucuronidase activity after 24 h, was dependent both on the expression and activation of both LpMPK2 and LpMPK3. These data suggest that the induction of extracellular invertase Lin6 by stress-related stimuli requires LpMPK2 and LpMPK3, and thus demonstrate that MAPK signalling might be involved in the regulation of primary carbon metabolism in general and sink metabolism in particular.
Additional keywords: GUS activity, Lycopersicon peruvianum, MAPK, sink metabolism.
Acknowledgements
TKH acknowledges the fellowship provided by DAAD and Graduierten Kolleg 1342. AKS and TR thanks funding from joint Indo-German DAAD-DST project (No. D/07/13475 and DST/INT/DAAD/P-165/2007).
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