Molecular cloning, expression and mapping of the translational initiation factor eIF1 gene in Oryza sativa
Latha Rangan A C , Anusuya Rout A , Medhavi Sudarshan A and Glenn Gregorio BA Department of Biotechnology, Indian Institute of Technology Guwahati, Assam 781 039, India.
B Plant Breeding, Genetics and Biochemistry Laboratory, International Rice Research Institute, DAPO BOX 7777, Metro Manila, The Philippines.
C Corresponding author. Email: latha_rangan@yahoo.com
Functional Plant Biology 36(5) 442-452 https://doi.org/10.1071/FP08276
Submitted: 28 October 2008 Accepted: 9 March 2009 Published: 6 May 2009
Abstract
Protein translation is very sensitive to salt stress and the proteins involved in this process may be an important determinant of salt tolerance. We isolated a rice cDNA clone (OseIF1) from a salt-tolerant indica cultivar (Pokkali) subjected to 150 mm NaCl, the deduced amino acid sequence of which had homology with the Sui1 suppressor locus in Saccharomyces cerevisiaei Hansen. The sequence contains 753 bp with an open-reading frame of 345 bp and shares similarity with the sequences of Sui1 and eIF1 in plants and mammals. Southern analysis indicates that the gene is present in more than a single copy per haploid genome and mapped to chromosome 1 of rice. Expression of the gene was increased by salt stress and also upregulated after exogenous ABA and mannitol treatments, suggesting that its induction is related to the water-deficit effect of high salt.
Additional keywords: abscisic acid, mannitol, rice, salt stress.
Acknowledgements
We thank the Directorate of the Rice Research Institute, Rajendranagar Hyderabad, and the Genetic Resources Center, International Rice Research Institute, The Philippines, for the kind supply of germplasm. We sincerely thank Dr TF Donahue, Indiana University, Bloomington, for kindly supplying the sui1 antibodies for the protein immunoblot assay. A. Rout and M. Sudarshan thank the Council of Scientific and Industrial Research (CSIR) for the award of a Research Fellowship. L. Rangan acknowledges funding from the CSIR, Government of India.
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