Physiological and biochemical assortment in different wheat genotypes (Triticum aestivum L.) under rain fed conditions
Syeda Okasha Javed A , Shahid Iqbal Awan A , Sania Shouket A , Kotb A. Attia
B , Zhu Xi C , Arif Ahmed Mohammed B , Sher Aslam Khan D , Sayeda Tanavish Javed E and Yasir Majeed A F *
A
B
C
D
E
F
Abstract
Wheat (Triticum aestivum L.) is the most extensively cultivated cereal crop in the world; however, its growth and development are affected by different types of biotic and abiotic stress conditions. The aim of this study was to assess the physico-chemical diversity in different wheat genotypes under rain-fed conditions. Principle component analysis (PCA) showed that significant variation for different components contributed 77.87% of total variability among all genotypes. In the scree plot, the first two PCs (PC1 = 44.75%, PC2 = 14.28%) had significant differences for numerous agronomic traits. The scatter biplot depicted eight genotypes (Zardana, NR-462, D-97, BARS-2009 (a check), NR-481, Tarnab-73, NR-489 and Pirsabak-91) with high diversity (variation ~90%) for different morphological traits, identifiable as they were located further away from the origin than other genotypes. Factor analysis of loading factors among wheat genotypes across different morpho-physiological traits also showed significant diversity for positive and negative loads. In cluster analysis, genotypes such as BWP-97, BARS-2009, NR-489, NR-448 and Pak. 13 were outliers, indicating significant diversity among all genotypes for different agronomic traits. Biochemical analysis showed maximum values for antioxidant activity, total phenolic content, and total flavonoid content in lines NR-485 (93.76%), NR-489 (3.55 mg gallic acid equivalent (GAE)/g), and the variety Suleman-96 (3.45 mg quercetin equivalent (QE)/g), respectively. This study provides new insights for understanding the diversity of different wheat genotypes under rain-fed conditions, and the selected genotypes can be evaluated for further breeding programs.
Keywords: biochemical analysis, diversity, factor analysis, genotypes, physiological traits, principle component analysis, rain-fed, wheat.
References
Abd El-Mohsen AA, Abd El-Shafi MA, Gheith EMS, Suleiman HS (2015) Using different statistical procedures for evaluating drought tolerance indices of bread wheat genotypes. Advance in Agriculture and Biology 4(1), 19-30.
| Google Scholar |
Abozed SS, El-Kalyoubi M, Abdelrashid A, Salama MF (2014) Total phenolic contents and antioxidant activities of various solvent extracts from whole wheat and bran. Annals of Agricultural Sciences 59, 63-67.
| Crossref | Google Scholar |
Amjadian E, Zeinodini A, Doğan H (2021) Effect of fertilizer management systems on growth and balance of nutrients in wheat cultivation. Central Asian Journal of Plant Science Innovations 1(2), 56-69.
| Google Scholar |
Asghar S, Rehman AU, Ahmad N, Ajmal S, Ahsan A, Gulnaz S, Javed M, Ahmad J, Iqbal J, Bibi S, Fiaz S, Elesawy BH, Askary AE, Ismail KA, Gharib AF, Mohyo-ud-Din A, Tabassum MI, Qayyum A (2022) Evaluation of Pakistani wheat germplasm for leaf rust resistance at various locations. PLoS ONE 17(5), e0266695.
| Crossref | Google Scholar | PubMed |
Baranwal DK, Mishra VK, Singh T (2013) Genetic diversity based on cluster and principal component analyses for yield and its contributing characters in wheat (Triticum aestivum L.). Madras Agricultural Journal 100(4–6), 320-323.
| Google Scholar |
Batool A, Noorka IR, Afzal M, Syed AH (2013) Estimation of heterosis, heterobeltiosis and potence ratio over environments among pre and post Green Revolution Spring wheat in Pakistan. Journal of Basic & Applied Sciences 9, 36-43.
| Crossref | Google Scholar |
Bhandari R, Gnawali S, Nyaupane S, Kharel S, Poudel M, Panth P (2021) Effect of drought & irrigated environmental condition on yield & yield attributing characteristic of bread wheat – a review. Reviews in Food and Agriculture 2(2), 59-62.
| Crossref | Google Scholar |
Bhatti N, Dalal M, Phougat D, Kavita S (2023) Multivariate analysis of morpho-physiological traits in bread wheat genotypes under terminal heat stress. Electronic Journal of Plant Breeding 13(4), 1180-1186.
| Crossref | Google Scholar |
Bonecke E, Breitsameter L, Bruggemann N, Chen T-W, Feike T, Kage H, Kersebaum K-C, Piepho H-P, Stützel H (2020) Decoupling of impact factors reveals the response of German winter wheat yields to climatic changes. Global Change Biology 26, 3601-3626.
| Crossref | Google Scholar | PubMed |
Chang C, Yang M, Wen H, Chern J (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis 10, 178-182.
| Google Scholar |
Dimitrov E, Uhr Z, Chipilski R (2022) Study of yield and stability by common winter wheat varieties by changing climatic conditions in Sadovo region. Bulgarian Journal of Agricultural Science 28(2), 271-278.
| Google Scholar |
Dixon J, Braun HJ, Kosina P, Crouch J (2009) ‘Wheat facts and futures 2009.’ (CIMMYT: Mexico D.F., Mexico) Available at https://repository.cimmyt.org/handle/10883/1265 [Accessed 6 December 2020]
Ebrahimnejad S, Rameeh V (2016) Correlation and factor analysis of grain yield and some important component characters in spring bread wheat genotypes. Cercetari Agronomice in Moldova 49(1), 5-15.
| Crossref | Google Scholar |
El Hassouni K, Afzal M, Steige KA, Sielaff M, Curella V, Neerukonda M, Tenzer S, Schuppan D, Longin CFH, Thorwarth P (2023) Multiomics based association mapping in wheat reveals genetic architecture of quality and allergenic related proteins. International Journal of Molecular Science 24, 1485.
| Crossref | Google Scholar |
Ghafoor K, Choi YN (2009) Optimization of ultrasound assisted extraction of phenolic compounds and antioxidants from grape peel through response surface methodology. Journal of the Korean Society for Applied Biology and Chemistry 52, 295-300.
| Crossref | Google Scholar |
Godfray HCJ, Beddington JR, Crute IR, Haddad L, Lawrence D, Muir JF, Robinson S, Thomas SM, Toulmin C (2010) Food security: the challenge of feeding 9 billion people. Science 327(5967), 812-818.
| Crossref | Google Scholar |
Hameed A, Sheikh MA, Jamil A, Basra SMA (2013) Seed priming with sodium silicate enhances seed germination and seedling growth in wheat (Triticum aestivum L.) under water deficit stress induced by polyethylene glycol. Pakistan Journal of Life Social Sciences 11(1), 19-24.
| Google Scholar |
Hammer Ø, Harper DAT, Ryan PD (2001) PAST: paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4(1), 9.
| Google Scholar |
Hussain A, Khan S, Liaqat S, Shafiullah K (2022) Developing evidence based policy and programmes in mountainous specific agriculture in Gilgit-Baltistan and Chitral regions of Pakistan. Pakistan Journal of Agricultural Research 35(1), 181-196.
| Google Scholar |
IDRC (2010) ‘Facts and figures on food and biodiversity.’ (IDRC Communications, International Development Research Centre: Canada) Available at https://www.idrc.ca/en/research-in-action/facts-figures-food-andbiodiversity
Janmohammadi M, Sabaghnia N, Nouraein M (2014) Path analysis of grain yield and yield components and some agronomic traits in bread wheat. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 62(5), 945-952.
| Crossref | Google Scholar |
Khan A, Khaliq I, Ahmad M, Ahmed HGMD, Khan AG, Farooq MS (2018) Comparative performance of spring wheat (Triticum aestivum L.) through heat stress indices. Pakistan Journal of Botany 50(2), 481-488.
| Google Scholar |
Khan T, Gul S, Khan NU, Fawibe OO, Akhtar N, Rehman M, Sabah N, Tahir MA, Iqbal A, Naz F, Haq I, Rauf A (2023) Stability analysis of wheat through genotype by environment interaction in three regions of Khyber Pakhtunkhwa, Pakistan. SABRAO Journal of Breeding and Genetics 55(1), 50-60.
| Crossref | Google Scholar |
Leilah AA, Al-Khateeb SA (2005) Statistical analysis of wheat yield under drought conditions. Journal of Arid Environments 61(3), 483-496.
| Crossref | Google Scholar |
Marrelli M, Sprovieri P, Conforti F, Statti G (2021) Phytochemical content and antioxidant activity of ancient Majorca and Carosella (Triticum aestivum L.) wheat flours. Agronomy 11, 1217.
| Crossref | Google Scholar |
Narayanan B, Soh P, Calhoun VD, Ruaño G, Kocherla M, Windemuth A, Clementz BA, Tamminga CA, Sweeney JA, Keshavan MS, Pearlson GD (2015) Multivariate genetic determinants of EEG oscillations in schizophrenia and psychotic bipolar disorder from the BSNIP study. Translational Psychiatry 5(6), e588.
| Crossref | Google Scholar | PubMed |
Pour-Aboughadareh A, Etminan A, Abdelrahman M, Siddique KHM, Tran L-SP (2020) Assessment of biochemical and physiological parameters of durum wheat genotypes at the seedling stage during polyethylene glycol-induced water stress. Plant Growth Regulation 92(1), 81-93.
| Crossref | Google Scholar |
Raj S, Brinkley C, Ulimwengu J (2022) Connected and extracted: Understanding how centrality in the global wheat supply chain affects global hunger using a network approach. PLoS ONE 17(6), e0269891.
| Crossref | Google Scholar |
Seher M, Shabbir G, Rasheed A, Kazi AG, Mahmood T, Mujeeb-Kazi A (2015) Performance of diverse wheat genetic stocks under moisture stress condition. Pakistan Journal of Botany 47(1), 21-26.
| Google Scholar |
Shahid Mukhtar M, Rahmanw M, Zafar Y (2002) Assessment of genetic diversity among wheat (Triticum aestivum L.) cultivars from a range of localities across Pakistan using random amplified polymorphic DNA (RAPD) analysis. Euphytica 128(3), 417-425.
| Crossref | Google Scholar |
Shiferaw B, Smale M, Braun HJ, Duveiller E, Reynolds M, Muricho G (2011) Crops that feed the world 10. Past successes and future challenges to the role played by wheat in global food security. Journal of Nutrition and Food Security 5, 291-317.
| Crossref | Google Scholar |
Sobhy SE, Aseel DG, Abo-Kassem E-EM, Sewelam NA, SaadAllah KM, Samy MA, Hafez EE (2023) Priming of wheat plant with weed extracts, calcium and salicylic acid for contribution to alleviating the oxidative stress imposed by Fusarium graminearum and lead toxicity. Novel Research in Microbiology Journal 7(2), 1932-1965.
| Crossref | Google Scholar |
Sofalian O, Chaparzadeh N, Javanmard A, Hejazi MS (2008) Study the genetic diversity of wheat landraces from northwest of Iran based on ISSR molecular markers. International Journal of Agriculture and Biology 10, 466-468.
| Google Scholar |
Wang C-Y, Chen Z-Z, Guo Y-X, Sun H-J, Zhang G-L, Kuang M-A, Yang S-X, Li X-M, Díaz de la Garza RI, Gou J-Y (2020) Isolation of wheat mutants with higher grain phenolics to enhance anti-oxidant potential. Food Chemistry 303, 125363.
| Crossref | Google Scholar | PubMed |
Waseem M, Ali Q, Ali A, Samiullah TR, Ahmad S, Baloch DM, Bajwa KS (2014) Genetic analysis for various traits of Cicer arietinum under different spacing. Life Science Journal 11(12s), 14-21.
| Google Scholar |
Yang D, Liu Y, Cheng H, Chang L, Chen J, Chai S, Li M (2016) Genetic dissection of flag leaf morphology in wheat (Triticum aestivum L.) under diverse water regimes. BMC Genet 17, 94.
| Crossref | Google Scholar |
Yu L, Perret J, Davy B, Wilson J, Melby CL (2002) Antioxidant properties of cereal products. Journal of Food Science 67(7), 2600-2603.
| Crossref | Google Scholar |
Zhang K, Zhang Y, Xu N, Yang X, Zhang G, Zhang Y, Liu Q (2020) Study of the protein, antioxidant activity, and starch during in vitro simulated digestion of green wheat and wheat cooked flours. International Journal of Food Properties 23, 722-735.
| Crossref | Google Scholar |
