Potassium accumulation characteristics and expression of related genes involved in potassium metabolism in a high-potassium variety: tobacco (Nicotiana tabacum) as a model
Zhi-Xiao Yang
A , Ying-Chao Lin A , Yi Cao A , Ren-Gang Wang A , De-Jun Kong A , Qian Hou B , Jian-Yu Gou C , Kaleem U. Kakar D , Ji-Shun Zhang A , Zhi-Hong Wang A * and Shi-Zhou Yu A *
+ Author Affiliations
- Author Affiliations
A Guizhou Academy of Tobacco Science, Guiyang 550081, China.
B College of Agronomy, Northwest A&F University, Yangling 712100, China.
C Zunyi Municipal Branch of Guizhou Tobacco Company, Zunyi 56300, China.
D Department of Biotechnology, Faculty of Life Sciences, Balochistan University of Information Technology, Engineering, and Management Sciences (BUITEMS), Quetta 87300, Pakistan.
Functional Plant Biology 49(10) 887-897 https://doi.org/10.1071/FP22011
Submitted: 18 January 2022 Accepted: 12 June 2022 Published: 8 July 2022
© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
We investigated potassium (K) accumulation characteristics and expression of K metabolism related genes in one high-K variety (ND202) and a common variety (NC89) of tobacco (Nicotiana tabacum L.). Results showed that K accumulation and leaf K content in ND202 were higher than those in NC89. The distribution rate and K accumulation in the leaves of ND202 increased significantly, while the distribution rate in the roots and stems had lower values. In addition, the maximum K accumulation rate and high-speed K accumulation duration in ND202 were found to be better than those in NC89. The expression of NKT1 in the upper and middle leaves of ND202 had an advantage, and the relative expression of NtKC1 and NtTPK1 in both the upper and middle leaves, as well as the roots, was also significantly upregulated. Conversely, the expression of NTRK1 in the lower leaves and roots of ND202 was weaker. ND202 had significantly greater expression levels of NtHAK1 than NC89 in the upper and middle leaves and roots; moreover, the expression of NtKT12 in the upper leaves and roots of ND202 was also higher. In comparison with common varieties, high-K varieties had a stronger ability to absorb and accumulate K. They also possessed higher expression of K+ channel- and transporter-related genes and showed a superior K accumulation rate and longer duration of high-speed K accumulation. Furthermore, K accumulation rate at 40–60 days can be suggested as an important reference for the selection of high-K tobacco varieties.
Keywords: accumulation, characteristic, gene expression, high-K variety, potassium, potassium metabolism, tobacco, utilisation.
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