Proteome changes and associated physiological roles in chickpea (Cicer arietinum) tolerance to heat stress under field conditions
Givemore M. Makonya A , John B. O. Ogola B , Hawwa Gabier C , Mohammed S. Rafudeen C , A. Muthama Muasya A , Olivier Crespo D , Sipho Maseko E , Alex J. Valentine
F , Carl-Otto Ottosen G , Eva Rosenqvist H and Samson B. M. Chimphango A *
+ Author Affiliations
- Author Affiliations
A Department of Biological Sciences, University of Cape Town, Private Bag X3, Rondebosch 7701, South Africa.
B Department of Plant Production, University of Venda, Private Bag X5050, Thohoyandou 0950, South Africa.
C Department of Molecular and Cell Biology, University of Cape Town, Private Bag, Rondebosch 7701, South Africa.
D Climate System Analysis Group, Environmental and Geographical Science Department, University of Cape Town, Rondebosch, Private Bag X3, Cape Town 7701, South Africa.
E Department of Crop Sciences, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa.
F Botany and Zoology Department, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa.
G Department of Food Science, Aarhus University, Kirstinebjergvej 10, 5792 Aarslev, Denmark.
H Department of Plant and Environmental Sciences, Section for Crop Science, University of Copenhagen, Hoejbakkegaard Allé 9, 2630 Taastrup, Denmark.
Functional Plant Biology 49(1) 13-24 https://doi.org/10.1071/FP21148
Submitted: 10 July 2020 Accepted: 8 October 2021 Published: 19 November 2021
© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
Interrogative proteome analyses are used to identify and quantify the expression of proteins involved in heat tolerance and to identify associated physiological processes in heat-stressed plants. The objectives of the study were to identify and quantify the expression of proteins involved in heat tolerance and to identify associated physiological processes in chickpea (Cicer arietinum L.) heat-tolerant (Acc#7) and sensitive genotype (Acc#8) from a field study. Proteomic and gene ontological analyses showed an upregulation in proteins related to protein synthesis, intracellular traffic, defence and transport in the heat-tolerant genotype compared to the susceptible one at the warmer site. Results from KEGG analyses indicate the involvement of probable sucrose-phosphate synthase (EC 2.4.1.14) and sucrose-phosphate phosphatase (EC 3.1.3.24) proteins, that were upregulated in the heat-tolerant genotype at the warmer site, in the starch and sucrose pathway. The presence of these differentially regulated proteins including HSP70, ribulose bisphosphate carboxylase/oxygenase activase, plastocyanin and protoporphyrinogen oxidase suggests their potential role in heat tolerance, at flowering growth stage, in field-grown chickpea. This observation supports unaltered physiological and biochemical performance of the heat-tolerant genotypes (Acc#7) relative to the susceptible genotype (Acc#8) in related studies (Makonya et al. 2019). Characterisation of the candidate proteins identified in the current study as well as their specific roles in the tolerance to heat stress in chickpea are integral to further crop improvement initiatives.
Keywords: chickpea, Cicer arietinum, field studies, flowering development, heat stress, heat tolerance, leaf proteomics, photosynthesis.
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