MIC-100, a new system for high-throughput phenotyping of instantaneous leaf photosynthetic rate in the field
Yu Tanaka
A E , Kazuki Taniyoshi A , Ayumu Imamura B , Ryo Mukai A , Shun Sukemura A , Kazuma Sakoda A C D and Shunsuke Adachi B
+ Author Affiliations
- Author Affiliations
A Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-chou, Sakyo-ku, Kyoto, 606-8502, Japan.
B College of Agriculture, Ibaraki University, 3-21-1 Chuo, Ami, Inashiki, Ibaraki 300-0393, Japan.
C Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Midori-cho, Nishitokyo, Tokyo 188-0002, Japan.
D Japan Society for the Promotion of Science, 5-3-1, Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan.
E Corresponding author. Email: tanaka.yu.2s@kyoto-u.ac.jp
Functional Plant Biology - https://doi.org/10.1071/FP21029
Submitted: 29 January 2021 Accepted: 4 May 2021 Published online: 7 June 2021
Abstract
Photosynthesis occurs mainly in plant leaves and is a fundamental process in the global carbon cycle and in crop production. The exploitation of natural genetic variation in leaf photosynthetic capacity is a promising strategy to meet the increasing demand for crops. The present study reports the newly developed photosynthesis measurement system ‘MIC-100,’ with a higher throughput for measuring instantaneous photosynthetic rate in the field. MIC-100 is established based on the closed system and directly detects the CO2 absorption in the leaf chamber. The reproducibility, accuracy, and measurement throughput of MIC-100 were tested using soybean (Glycine max L. (Merr.)) and rice (Oryza sativa L.) grown under field conditions. In most cases, the coefficient of variance (CV) for repeated-measurements of the same leaf was less than 0.1. The photosynthetic rates measured with the MIC-100 model showed a significant correlation (R2 = 0.93–0.95) with rates measured by a widely used gas-exchange system. The measurement throughput of the MIC-100 is significantly greater than that of conventional open gas-exchange systems under field conditions. Although MIC-100 solely detects the instantaneous photosynthetic rate under a given environment, this study demonstrated that the MIC-100 enables the rough evaluation of leaf photosynthesis within the large-scale plant populations grown in the field.
Keywords: crop production, photosynthesis, MIC-100, photosynthetic rate measurement, Glycine max, Oryza sativa, rice, soybean, open gas-exchange system.
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