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Plant function and evolutionary biology
RESEARCH ARTICLE

Changes in crassulacean acid metabolism expression, chloroplast ultrastructure, photochemical and antioxidant activity in the Aloe vera during acclimation to combined drought and salt stress

Ghader Habibi https://orcid.org/0000-0002-3624-2701 A *
+ Author Affiliations
- Author Affiliations

A Department of Biology, Payame Noor University (PNU), PO BOX 19395-3697 Tehran, Iran.

* Correspondence to: gader.habibi@gmail.com

Handling Editor: Ismail Turkan

Functional Plant Biology 49(1) 40-53 https://doi.org/10.1071/FP21008
Submitted: 7 January 2021  Accepted: 18 October 2021   Published: 16 November 2021

© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing

Abstract

We determined time course changes of photochemical and antioxidant activity during the induction of strong crassulacean acid metabolism (CAM) in Aloe vera L. plants grown under salt and drought stress. We found that the strong CAM was induced during 25–30 days of drought alone treatment. After 25–30 days, we showed the withdrawal of strong CAM back to constitutive CAM background under the combination of simultaneous drought and salt stress, which coincided with the accumulation of malondialdehyde, and the decrease in the contents of endogenous nitric oxide (NO) and non-enzymatic antioxidants. At the same time, the chloroplast ultrastructure was damaged with a parallel accumulation of reactive oxygen species, and the whole photosynthetic electron transport flux was impaired by combined stress treatment. In conclusion, the changes in CAM expression parameters was attended by a similar pattern of antioxidant and photochemical change in Aloe plants subjected to only drought or combined stress.

Keywords: Aloe vera L., antioxidant status, CAM expression, drought stress, nitric oxide, photochemical functioning, photosynthetic electron transport, salt stress.


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