The labile zinc pool in plant cells
Ilya E. Zlobin
A B , Alexander V. Kartashov A , Alexander V. Nosov A , Artem A. Fomenkov A and Vladimir V. Kuznetsov A
+ Author Affiliations
- Author Affiliations
A Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276 Moscow, Russia.
B Corresponding author. Email: ilya.zlobin.90@mail.ru
Functional Plant Biology 46(9) 796-805 https://doi.org/10.1071/FP19064
Submitted: 26 February 2019 Accepted: 18 April 2019 Published: 10 May 2019
Abstract
Zinc is the most abundant and important transition metal in plants; however, the dynamic aspects of zinc homeostasis in plant cells are poorly understood. In this study we explored the pool of labile exchangeable zinc complexes in plant cells, and the potential influence of changes in intracellular zinc availability on cellular physiology. Work was performed on cultivated cell extracts of Arabidopsis thaliana (L.) Heynh. and Thellungiella salsuginea (Pall.) O.E. Schulz grown under control (3.48 µM Zn2+), 10-fold Zn excess or Zn starvation conditions. The free and labile Zn contents in the extracts were then determined by fluorimetric titration. We observed for the first time that plant cells contain micromolar concentrations of labile zinc complexes that account for a low percentage of the total zinc content. Labile zinc is mainly protein bound. Zn starvation inhibits cell proliferation and leads to the disappearance of the labile zinc pool, whereas Zn excess drastically increases the labile zinc pool. Free Zn2+ is buffered at picomolar concentrations in the intracellular milieu, and the increase in free Zn2+ concentrations to low nanomolar values clearly modulates enzyme activity by direct reversible binding. Such increases in free Zn2+ can be achieved by the substantial influx of additional zinc or by the oxidation of zinc-binding thiols. The observed features of the labile zinc pool in plant cells suggest it has a role in intracellular zinc trafficking and zinc signalling.
Additional keywords: enzyme activity, phosphatase, plant signalling, trace metals, zinc metabolism.
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