Distinction and characterisation of rice genotypes tolerant to combined stresses of salinity and partial submergence, proved by a high-resolution chlorophyll fluorescence imaging system
Bhubaneswar Pradhan A B , Koushik Chakraborty A , Nibedita Prusty A , DeepaA,C , Arup Kumar Mukherjee A , Krishnendu Chattopadhyay A and Ramani Kumar Sarkar A D
+ Author Affiliations
- Author Affiliations
A Indian Council of Agricultural Research – National Rice Research Institute, Cuttack, Odisha 753 006, India.
B Present address: Indian Council of Agricultural Research – Indian Institute of Agricultural Biotechnology, Ranchi-8340101, India.
C Present address: Biomedical Engineering, Chung Yuan Christian University, Zhongli, 320, Taiwan.
D Corresponding author. Email: rksarkarcrri@gmail.com; rk.sarkar@icar.gov.in
Functional Plant Biology 46(3) 248-261 https://doi.org/10.1071/FP18157
Submitted: 18 June 2018 Accepted: 2 October 2018 Published: 12 November 2018
Abstract
Chlorophyll a fluorescence (ChlF) parameters measured with fluorescence imaging techniques were used to investigate the combined effect of salt and partial submergence stress to understand photosynthetic performance in rice (Oryza sativa L.). ChlF parameters such as maximal fluorescence (Fm), variable fluorescence (Fv = Fm –F0), the maximal photochemical efficiency of PSII (Fv/Fm) and the quantum yield of nonregulated energy dissipation of PSII (Y(NO)) were able to distinguish genotypes precisely based on their sensitivity to stress. Upon analysis, we found the images of F0 were indistinguishable among the genotypes, irrespective of their tolerance to salt and partial submergence stress. On the contrary, the images of Fm and Fv/Fm showed marked differences between the tolerant and susceptible genotypes in terms of tissue greenness and the appearance of dark spots as stress symptoms. The images of effective PSII quantum yield, the coefficient of nonphotochemical quenching (qN) and the coefficient of photochemical quenching (qP) captured under different PAR were able to distinguish the tolerant and susceptible genotypes, and were also quite effective for differentiating the tolerant and moderately tolerant ones. Similarly, the values of electron transport rate, qN, qP and Y(NO) were also able to distinguish the genotypes based on their sensitivity to stress. Overall, this investigation indicates the suitability of chlorophyll fluorescence imaging technique for precise phenotyping of rice based on their sensitivity to the combined effect of salt and partial submergence.
Additional keywords: Fv/Fm, phenotyping, photosystem II.
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