Optimising the linear electron transport rate measured by chlorophyll a fluorescence to empirically match the gross rate of oxygen evolution in white light: towards improved estimation of the cyclic electron flux around photosystem I in leaves
Meng-Meng Zhang A B , Da-Yong Fan B , Guang-Yu Sun A C and Wah Soon Chow B C
+ Author Affiliations
- Author Affiliations
A College of Life Science, Northeast Forestry University, Harbin, Heilongjiang, 150040, China.
B Division of Plant Sciences, Research School of Biology, The Australian National University, Acton, ACT 2601, Australia.
C Corresponding authors. Email: fred.chow@anu.edu.au; sungy@vip.sina.com
Functional Plant Biology 45(11) 1138-1148 https://doi.org/10.1071/FP18039
Submitted: 14 February 2018 Accepted: 25 May 2018 Published: 3 July 2018
Abstract
The cyclic electron flux (CEF) around photosystem I (PSI) was discovered in isolated chloroplasts more than six decades ago, but its quantification has been hampered by the absence of net formation of a product or net consumption of a substrate. We estimated in vivo CEF in leaves as the difference (ΔFlux) between the total electron flux through PSI (ETR1) measured by a near infrared signal, and the linear electron flux through both photosystems by optimised measurement of chlorophyll a fluorescence (LEFfl). Chlorophyll fluorescence was excited by modulated green light from a light-emitting diode at an optimal average irradiance, and the fluorescence was detected at wavelengths >710 nm. In this way, LEFfl matched the gross rate of oxygen evolution multiplied by 4 (LEFO2) in broad-spectrum white actinic irradiance up to half (spinach, poplar and rice) or one third (cotton) of full sunlight irradiance. This technique of estimating CEF can be applied to leaves attached to a plant.
Additional keywords: light partitioning, P700, photochemical quenching, photosystem II.
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