Fructan dynamics and antioxidant capacity of 4-day-old seedlings of wheat (Triticum aestivum) cultivars during drought stress and recovery
Farnoosh Nemati A , Faezeh Ghanati A C , Hassan Ahmadi Gavlighi B and Mohsen Sharifi A
+ Author Affiliations
- Author Affiliations
A Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran.
B Department of Food Science and Technology, Faculty of Agriculture, Tarbiat Modares University (TMU), POB14115-336, Tehran, Iran.
C Corresponding author. Email: ghangia@modares.ac.ir
Functional Plant Biology 45(10) 1000-1008 https://doi.org/10.1071/FP18008
Submitted: 21 August 2017 Accepted: 24 March 2018 Published: 8 May 2018
Abstract
One of the inevitable consequences of drought stress is enhanced production of reactive oxygen species (ROS). Fructan might function as effective candidate for capturing ROS in a wide range of stresses. Herein, 4-day-old seedlings of drought-tolerant and -sensitive wheat cultivars were exposed to drought stress for 7 days by water cessation, followed by further 7 days re-watering. The content, metabolism, related enzymes activity, degree of polymerisation (DP) and antioxidant capacity of fructan were compared in the two cultivars. High resolution HPAEC-PAD analysis of fructan showed an increase in the activities of fructan: fructan 1-fructosyltransferase (1-FFT) in the tolerant cultivar and sucrose: sucrose 1-fructosyltransferase (1-SST) and 1-FFT in the sensitive cultivar under drought condition. The activity of fructan exohydrolase (FEH) did not show any significant change in tolerant cultivar, but decreased in a sensitive one. In comparison with the sensitive cultivar, the tolerant one accumulated fructan (0.9% of dry matter) with higher degree of polymerisation (10.67 ± 1.1), accompanied by increased OH radical scavenging activity, during drought condition. In regard to the fact that OH radical is the most prevalent ROS in damaging membrane lipids, the results suggest that fructans play a crucial role in the tolerance of wheat seedlings against drought stress.
Additional keywords: fructan exohydrolase, fructan: fructan 1-fructosyltransferase, ROS, sucrose, sucrose 1-fructosyltransferase, water cessation.
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