The effect of simulated microgravity on the Brassica napus seedling proteome
Andrej Frolov A * , Anna Didio A B * , Christian Ihling C , Veronika Chantzeva D , Tatyana Grishina B , Wolfgang Hoehenwarter E , Andrea Sinz C , Galina Smolikova D , Tatiana Bilova A D F and Sergei Medvedev D F
+ Author Affiliations
- Author Affiliations
A Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, DE 06120, Halle/Saale, Germany.
B Department of Biochemistry, St. Petersburg State University, RU 199034, St. Petersburg, Russian Federation.
C Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Martin-Luther Universität Halle-Wittenberg, DE 06120, Halle/Saale, Germany.
D Department of Plant Physiology and Biochemistry, St. Petersburg State University, RU 199034, St. Petersburg, Russian Federation.
E Proteome Analytics Research Group, Leibniz Institute of Plant Biochemistry, DE 06120, Halle/Saale, Germany.
F Corresponding authors. Emails: bilova.tatiana@gmail.com; s.medvedev@spbu.ru
Functional Plant Biology 45(4) 440-452 https://doi.org/10.1071/FP16378
Submitted: 31 October 2016 Accepted: 5 October 2017 Published: 7 December 2017
Abstract
The magnitude and the direction of the gravitational field represent an important environmental factor affecting plant development. In this context, the absence or frequent alterations of the gravity field (i.e. microgravity conditions) might compromise extraterrestrial agriculture and hence space inhabitation by humans. To overcome the deleterious effects of microgravity, a complete understanding of the underlying changes on the macromolecular level is necessary. However, although microgravity-related changes in gene expression are well characterised on the transcriptome level, proteomic data are limited. Moreover, information about the microgravity-induced changes in the seedling proteome during seed germination and the first steps of seedling development is completely missing. One of the valuable tools to assess gravity-related issues is 3D clinorotation (i.e. rotation in two axes). Therefore, here we address the effects of microgravity, simulated by a two-axial clinostat, on the proteome of 24- and 48-h-old seedlings of oilseed rape (Brassica napus L.). The liquid chromatography-MS-based proteomic analysis and database search revealed 95 up- and 38 downregulated proteins in the tryptic digests obtained from the seedlings subjected to simulated microgravity, with 42 and 52 annotations detected as being unique for 24- and 48-h treatment times, respectively. The polypeptides involved in protein metabolism, transport and signalling were annotated as the functional groups most strongly affected by 3-D clinorotation.
Additional keywords: 3D clinorotation, LC-MS, proteomics, seedling development, seed germination.
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