Rapid changes in root HvPIP2;2 aquaporins abundance and ABA concentration are required to enhance root hydraulic conductivity and maintain leaf water potential in response to increased evaporative demand
Dmitry S. Veselov A , Guzel V. Sharipova A , Stanislav Yu. Veselov B , Ian C. Dodd C , Igor Ivanov A and Guzel R. Kudoyarova A D
+ Author Affiliations
- Author Affiliations
A Ufa Institute of Biology of Russian Academy of Sciences, pr. Octyabrya, 69, Ufa 450 054, Russia.
B Biological Faculty of Bashkir State University, Validi, 32, Ufa 450 073, Russia.
C Lancaster Environment Centre, Lancaster University, Bailrigg, Lancashire LA1 4YQ, UK.
D Corresponding author. Email: guzel@anrb.ru
This paper originates from a presentation at the Fourth International Symposium on Plant Signaling and Behavior, Komarov Botanical Institute RAS/Russian Science Foundation, Saint Petersburg, Russia, 19–23 June 2016.
Functional Plant Biology 45(2) 143-149 https://doi.org/10.1071/FP16242
Submitted: 13 July 2016 Accepted: 23 September 2016 Published: 9 November 2016
Abstract
To address the involvement of abscisic acid (ABA) in regulating transpiration and root hydraulic conductivity (LpRoot) and their relative importance for maintaining leaf hydration, the ABA-deficient barley mutant Az34 and its parental wild-type (WT) genotype (cv. Steptoe) were grown in hydroponics and exposed to changes in atmospheric vapour pressure deficit (VPD) imposed by air warming. WT plants were capable of maintaining leaf water potential (ψL) that was likely due to increased LpRoot enabling higher water flow from the roots, which increased in response to air warming. The increased LpRoot and immunostaining for HvPIP2;2 aquaporins (AQPs) correlated with increased root ABA content of WT plants when exposed to increased air temperature. The failure of Az34 to maintain ψL during air warming may be due to lower LpRoot than WT plants, and an inability to respond to changes in air temperature. The correlation between root ABA content and LpRoot was further supported by increased root hydraulic conductivity in both genotypes when treated with exogenous ABA (10−5 M). Thus the ability of the root system to rapidly regulate ABA levels (and thence aquaporin abundance and hydraulic conductivity) seems important to maintain leaf hydration.
Additional keywords: absicisic acid, Hordeum vulgare, tissue hydration, water relations.
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