Expression of a Nicotiana tabacum pathogen-induced gene is involved in the susceptibility to black shank
Roxana Portieles A , Eduardo Canales A , Osmani Chacon B , Yussuan Silva B , Ingrid Hernández A , Yunior López A , Mayra Rodríguez A , Ryohei Terauchi C , Hideo Matsumura D , Carlos Borroto E , Jonathan D. Walton F , Ramon Santos G and Orlando Borrás-Hidalgo A H
+ Author Affiliations
- Author Affiliations
A Centre for Genetic Engineering and Biotechnology, PO Box 6162, Havana, 10 600, Cuba.
B Tobacco Research Institute, Carretera de Tumbadero 8, PO Box 6063, Havana, Cuba.
C Iwate Biotechnology Research Centre, Kitakami, Iwate, 024-0003, Japan.
D Gene Research Center, Shinshu University, Ueda 386-8567, Japan.
E Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Colonia Chuburná de Hidalgo, 97200 Mérida, Yucatán, México.
F Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, USA 48824.
G Centro de Bioplantas, Carretera de Morón Km 9, Ciego de Avila, C. P. 69450, Cuba.
H Corresponding author. Email: orlando.borras@cigb.edu.cu
Functional Plant Biology 43(6) 534-541 https://doi.org/10.1071/FP15350
Submitted: 11 November 2015 Accepted: 22 March 2016 Published: 9 May 2016
Abstract
Many host genes induced during compatible plant–pathogen interactions constitute targets of pathogen virulence factors that act to suppress host defenses. In order to identify Nicotiana tabacum L. genes for pathogen-induced proteins involved in susceptibility to the oomycete Phytophthora parasitica var. nicotianae, we used SuperSAGE technology combined with next-generation sequencing to identify transcripts that were differentially upregulated during a compatible interaction. We identified a pathogen-induced gene (NtPIP) that was rapidly induced only during the compatible interaction. Virus-induced gene silencing of NtPIP reduced the susceptibility of N. tabacum to P. parasitica var. nicotianae. Additionally, transient expression of NtPIP in the resistant species Nicotiana megalosiphon Van Heurck & Mull. Arg. compromised the resistance to P. parasitica var. nicotianae. This pathogen-induced protein is therefore a positive regulator of the susceptibility response against an oomycete pathogen in tobacco.
Additional keywords: oomycete, SuperSAGE.
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