Comparative proteomic and physiological characterisation of two closely related rice genotypes with contrasting responses to salt stress
Seyed Abdollah Hosseini A , Javad Gharechahi B , Manzar Heidari A , Parisa Koobaz A , Shapour Abdollahi A , Mehdi Mirzaei C , Babak Nakhoda A E and Ghasem Hosseini Salekdeh D E
+ Author Affiliations
- Author Affiliations
A Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran, PO Box 31535-1897, Karaj 3135933151, Iran.
B Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, PO Box 19395-5478, Tehran 1435916471, Iran.
C Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia.
D Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, PO Box 31535-1897, Karaj 3135933151, Iran.
E Corresponding authors. Emails: h_salekdeh@abrii.ac.ir; b.nakhoda@abrii.ac.ir
Functional Plant Biology 42(6) 527-542 https://doi.org/10.1071/FP14274
Submitted: 30 September 2014 Accepted: 7 February 2015 Published: 26 March 2015
Abstract
Salinity is a limiting factor affecting crop growth. We evaluated the responses of a salt-tolerant recombinant inbred rice (Oryza sativa L.) line, FL478, and the salt-sensitive IR29. Seedlings were exposed to salt stress and the growth rate was monitored to decipher the effect of long-term stress. At Day 16, IR29 produced lower shoot biomass than FL478. Significant differences for Na+ and K+ concentrations and Na+ : K+ ratios in roots and shoots were observed between genotypes. Changes in the proteomes of control and salt-stressed plants were analysed, identifying 59 and 39 salt-responsive proteins in roots and leaves, respectively. Proteomic analysis showed greater downregulation of proteins in IR29. In IR29, proteins related to pathways involved in salt tolerance (e.g. oxidative stress response, amino acid biosynthesis, polyamine biosynthesis, the actin cytoskeleton and ion compartmentalisation) changed to combat salinity. We found significant downregulation of proteins related to photosynthetic electron transport in IR29, indicating that photosynthesis was influenced, probably increasing the risk of reactive oxygen species formation. The sensitivity of IR29 might be related to its inability to exclude salt from its transpiration stream, to compartmentalise excess ions and to maintain a healthy photosynthetic apparatus during salt stress, or might be because of the leakiness of its roots, allowing excess salt to enter apoplastically. In FL478, superoxide dismutase, ferredoxin thioredoxin reductase, fibre protein and inorganic pyrophosphatase, which may participate in salt tolerance, increased in abundance. Our analyses provide novel insights into the mechanisms behind salt tolerance and sensitivity in genotypes with close genetic backgrounds.
Additional keywords: 2D gel electrophoresis, mass spectrometry, Oryza sativa, salinity, sensitivity, tolerance.
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