Dynamic carbon allocation into source and sink tissues determine within-plant differences in carbon isotope ratios
Frederik Wegener A B C , Wolfram Beyschlag B and Christiane Werner A
+ Author Affiliations
- Author Affiliations
A AgroEcosystem Research, BAYCEER, University of Bayreuth, Universitätsstrasse 30, 95447 Bayreuth, Germany.
B Experimental and Systems Ecology, University of Bielefeld, Universitätsstrasse 25, 33615 Bielefeld, Germany.
C Corresponding author. Email: frederik.wegener@uni-bayreuth.de
Functional Plant Biology 42(7) 620-629 https://doi.org/10.1071/FP14152
Submitted: 4 June 2014 Accepted: 10 March 2015 Published: 16 April 2015
Abstract
Organs of C3 plants differ in their C isotopic signature (δ13C). In general, leaves are 13C-depleted relative to other organs. To investigate the development of spatial δ13C patterns, we induced different C allocation strategies by reducing light and nutrient availability for 12 months in the Mediterranean shrub Halimium halimifolium L. We measured morphological and physiological traits and the spatial δ13C variation among seven tissue classes during the experiment. A reduction of light (Low-L treatment) increased aboveground C allocation, plant height and specific leaf area. Reduced nutrient availability (Low-N treatment) enhanced C allocation into fine roots and reduced the spatial δ13C variation. In contrast, control and Low-L plants with high C allocation in new leaves showed a high δ13C variation within the plant (up to 2.5‰). The spatial δ13C variation was significantly correlated with the proportion of second-generation leaves from whole-plant biomass (R2 = 0.46). According to our results, isotope fractionation in dark respiration can influence the C isotope composition of plant tissues but cannot explain the entire spatial pattern seen. Our study indicates a foliar depletion in 13C during leaf development combined with export of relatively 13C-enriched C by mature source leaves as an important reason for the observed spatial δ13C pattern.
Additional keywords: growth, Halimium halimifolium L., photosynthetic 13C discrimination, stable isotopes.
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