Post-anthesis heat and a Gpc-B1 introgression have similar but non-additive effects in bread wheat
Lancelot Maphosa A B C , Nicholas C. Collins A B , Julian Taylor B and Diane E. Mather A B D
+ Author Affiliations
- Author Affiliations
A Australian Centre for Plant Functional Genomics, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia.
B School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, Australia.
C Present address: Department of Environment and Primary Industries, 110 Natimuk Road, Horsham, Vic. 3400, Australia.
D Corresponding author. Email: diane.mather@adelaide.edu.au
Functional Plant Biology 41(9) 1002-1008 https://doi.org/10.1071/FP14060
Submitted: 22 February 2014 Accepted: 8 May 2014 Published: 10 June 2014
Journal Compilation © CSIRO Publishing 2014 Open Access CC BY-NC-ND
Abstract
High temperatures during grain filling can reduce the yield of wheat and affect its grain protein concentration. The Gpc-B1 locus of wheat also affects grain protein concentration, but it is not known whether its effects interact with those of heat. The aim of this study was to investigate the effects of high temperature in lines with and without functional (high-protein) alleles at Gpc-B1. A highly replicated experiment was conducted in a glasshouse under control conditions (24/18°C, 14/10 h day/night), with half of the plants of each line or cultivar put into a heat chamber (37/27°C, 14/10 h day/night) at 15 days after anthesis for 3 days. Backcross derivatives with the Gpc-B1 introgression segment differed from their recurrent parents more than those without that segment. In some respects, the effects of the Gpc-B1 introgression were similar to those of the heat treatment: both could accelerate peduncle senescence, increase grain protein content and increase the percentage of unextractable polymeric protein. Unlike the heat treatment, Gpc-B1 did not reduce grain weight, indicating that factors that hasten senescence do not necessarily limit grain size. The presence of the Gpc-B1 segment did not exacerbate the effects of heat stress on any trait.
Additional keywords: protein, senescence, single nucleotide polymorphism, stress.
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