Identification and characterisation of a novel class I endo-β-1,3-glucanase regulated by salicylic acid, ethylene and fungal pathogens in strawberry
Martín G. Martínez Zamora A C , Carlos Grellet Bournonville A C , Atilio P. Castagnaro B and Juan C. Díaz Ricci A D
+ Author Affiliations
- Author Affiliations
A INSIBIO (CONICET-UNT) – Departamento de Bioquímica de la Nutrición e Instituto de Qca Biológica ‘Dr Bernabé Bloj’, Facultad de Bioquímica, Química y Farmacia (UNT), Chacabuco 461 (4000) Tucumán, Argentina.
B Sección Biotecnología, Estación Experimental Agroindustrial O. Colombres-Unidad asociada al INSIBIO, CC No. 9 (4101) Las Talitas, Tucumán, Argentina.
C These authors contributed equally to this work.
D Corresponding author. Email: juan@fbqf.unt.edu.ar
Functional Plant Biology 39(5) 412-420 https://doi.org/10.1071/FP11275
Submitted: 14 December 2011 Accepted: 20 March 2012 Published: 24 April 2012
Abstract
The identification of a full length cDNA encoding an endo-β-1,3-glucanase (FaOGBG-5) from strawberry (Fragaria × ananassa Duch) is reported. The analysis of the deduced amino acid sequence of FaOGBG-5 showed that it shares typical structural features and a high degree of identity with other plant β-1,3-glucanases of the class I. The expression of FaOGBG-5 in plants infected with a virulent isolate of Colletotrichum acutatum and an avirulent isolate of Colletotrichum fragariae was examined. Induction of expression was observed with both pathogens but exhibited a delayed high expression with the virulent one. Additionally, the accumulation of FaOGBG-5 transcripts was also observed after treatments with the stress related hormones salicylic acid and ethylene. Results obtained suggest that the β-1,3-glucanase encoded by FaOGBG-5 may be implicated in plant defence against biotic and abiotic stress.
Additional keywords: plant defence, β-1,3-glucanases.
References
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. Journal of Molecular Biology 215, 403–410.Brown RL, Kazan K, McGrath KC, Maclean DJ, Manners JM (2003) A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis. Plant Physiology 132, 1020–1032.
| A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXkslertbg%3D&md5=a15c50eff617e77db24f43eeeba6c438CAS |
Casado-Díaz A, Encinas-Villarejo E, de los Santos B, Schilirò E, Yubero-Serrano EM, Amil-Ruíz F, Pocovi MI, Pliego-Alfaro F, Dorado G, Rey M, Romero F, Muñoz-Blanco J, Caballero JL (2006) Analysis of strawberry genes differentially expressed in response to Colletotrichum infection. Physiologia Plantarum 128, 633–650.
| Analysis of strawberry genes differentially expressed in response to Colletotrichum infection.Crossref | GoogleScholarGoogle Scholar |
Chalfoun NR, Castagnaro AP, Díaz Ricci JC (2005) Fungal elicitor induces hypersensitive response and systemic oxidative burst in strawberry plants. Biocell 29, 190
Conrath U (2006) Systemic acquired resistance. Plant Signaling & Behavior 1, 179–184.
| Systemic acquired resistance.Crossref | GoogleScholarGoogle Scholar |
Delp BR, Milholland RD (1980) Evaluating strawberry plants for resistance to Colletotrichum fragariae. Plant Disease 64, 1071–1073.
| Evaluating strawberry plants for resistance to Colletotrichum fragariae.Crossref | GoogleScholarGoogle Scholar |
Emanuelsson O, Brunak S, von Heijne G, Nielsen H (2007) Locating proteins in the cell using TargetP, SignalP, and related tools. Nature Protocols 2, 953–971.
| Locating proteins in the cell using TargetP, SignalP, and related tools.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtFGnur%2FF&md5=ea6ca23886084c7a56fb2d3bc31e055bCAS |
Felsenstein J (1985) Confidence limits on phylogenesis: an approach using the bootstrap. Evolution 39, 783–791.
| Confidence limits on phylogenesis: an approach using the bootstrap.Crossref | GoogleScholarGoogle Scholar |
Iandolino AB, Goes da Silva F, Lim H, Choi H, Willams LE, Cook DR (2004) High-quality RNA, cDNA, and derived EST libraries from grapevine (Vitis vinifera L.). Plant Molecular Biology Reporter 22, 269–278.
| High-quality RNA, cDNA, and derived EST libraries from grapevine (Vitis vinifera L.).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhs1yqsLc%3D&md5=43f8649cc875503dfb0701178048712fCAS |
Jones JDG, Dangl JL (2006) The plant immune system. Nature 444, 323–329.
| The plant immune system.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xht1SgtbzO&md5=97b4c1db82476bef96962cdb19700970CAS |
Kliebenstein DJ, Rowe HC (2008) Ecological costs of biotrophic versus necrotrophic pathogen resistance, the hypersensitive response and signal transduction. Plant Science 174, 551–556.
| Ecological costs of biotrophic versus necrotrophic pathogen resistance, the hypersensitive response and signal transduction.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXlslynsL8%3D&md5=659c4125708849ba50c0ae4c4042ca5cCAS |
Kumar S, Tamura K, Jakobsen IB, Nei M (2001) ‘MEGA2: Molecular Evolutionary Genetics Analysis software.’ (Arizona State University: Tempe, AZ)
Leubner-Metzger G, Meins F Jr (1999) Functions and regulation of plant β-1,3-glucanases (PR-2). In ‘Pathogenesis-related proteins in plants’. (Eds SK Datta, S Mathukrishnan) pp. 49–76. (CRC Press: Boca Raton, FL, USA)
Marchler-Bauer A, Bryant SH (2004) CD-Search: protein domain annotations on the fly. Nucleic Acids Research 32, W327–W331.
| CD-Search: protein domain annotations on the fly.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXlvFKntb4%3D&md5=105ec1cacbc67d0e9773ebdfc3b1ef59CAS |
Martínez Zamora MG, Castagnaro AP, Diaz Ricci JC (2004) Isolation and diversity analysis of resistance gene analogues (RGAs) from cultivated and wild strawberries. Molecular Genetics and Genomics 272, 480–487.
| Isolation and diversity analysis of resistance gene analogues (RGAs) from cultivated and wild strawberries.Crossref | GoogleScholarGoogle Scholar |
Martínez Zamora MG, Castagnaro AP, Díaz Ricci JC (2008) Genetic diversity of Pto-like serine/threonine kinase disease resistance genes in cultivated and wild strawberries. Journal of Molecular Evolution 67, 211–221.
| Genetic diversity of Pto-like serine/threonine kinase disease resistance genes in cultivated and wild strawberries.Crossref | GoogleScholarGoogle Scholar |
Münch-Garthoff S, Neuhaus J-M, Boller T, Kemmerling B, Kogel K-H (1997) Expression of β-1, 3-glucanase and chitinase in healthy, stem-rust-affected and elicitortreated near-isogenic wheat lines showing Sr5- or Sr24-specified race-specific rust resistance. Planta 201, 235–244.
| Expression of β-1, 3-glucanase and chitinase in healthy, stem-rust-affected and elicitortreated near-isogenic wheat lines showing Sr5- or Sr24-specified race-specific rust resistance.Crossref | GoogleScholarGoogle Scholar |
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473–497.
| A revised medium for rapid growth and bioassays with tobacco tissue cultures.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF3sXksFKm&md5=653cbff4216b152d155a6572062308d2CAS |
Pflieger S, Palloix A, Caranta C, Blattes A, Lefebvre V (2001) Defense response genes co-localize with quantitative disease resistance loci in pepper. Theoretical and Applied Genetics 103, 920–929.
| Defense response genes co-localize with quantitative disease resistance loci in pepper.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XivVOguw%3D%3D&md5=166dce4cb9ec2ce61946cfd094433acdCAS |
Philip S, Joseph A, Kumar A, Jacob C, Kothandaraman R (2001) Detection of β-1,3-glucanase isoforms against Corynespora leaf disease of rubber (Hevea brasiliensis). Indian Journal of Natural Rubber Research 14, 1–6.
Richardson PT, Baker DA, Ho LC (1982) The chemical composition of cucurbit vascular exudates. Journal of Experimental Botany 33, 1239–1247.
| The chemical composition of cucurbit vascular exudates.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL3sXkvVGjt7g%3D&md5=d909e1842d47dbeb0cd57ce86e1de2a8CAS |
Ron M, Avni A (2004) The receptor for the fungal elicitor ethylene-inducing xylanase is a member of a resistance-like gene family in tomato. The Plant Cell 16, 1604–1615.
| The receptor for the fungal elicitor ethylene-inducing xylanase is a member of a resistance-like gene family in tomato.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXlsFWlsbg%3D&md5=722e89649834f850660507289cc7cb0bCAS |
Rushton PJ, Torres JT, Parniske M, Wernert P, Hahlbrock K, Somssich IE (1996) Interaction of elicitor-induced DNA-binding proteins with elicitor response elements in the promoters of parsley PR-1 genes. The EMBO Journal 15, 5690–5700.
Salazar SM, Castagnaro AP, Arias ME, Chalfoun N, Tonello U, Díaz Ricci JC (2007) Induction of a defense response in strawberry mediated by an avirulent strain of Colletotrichum. European Journal of Plant Pathology 117, 109–122.
| Induction of a defense response in strawberry mediated by an avirulent strain of Colletotrichum.Crossref | GoogleScholarGoogle Scholar |
Shi Y, Zhang Y, Shih DS (2006) Cloning and expression analysis of two β-1,3-glucanase genes from strawberry. Journal of Plant Physiology 163, 956–967.
| Cloning and expression analysis of two β-1,3-glucanase genes from strawberry.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtFals7bK&md5=3c618e6922ca8a0b5b93d30314f53121CAS |
Singh PK, Chaturvedi VK, Singh HB (2011) Cross talk signalling: an emerging defense strategy in plants. Current Science 100, 288–289.
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 4876–4882.
| The CLUSTAL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXntFyntQ%3D%3D&md5=a0c7e54e30d37bd2fa3a93d66e591a25CAS |
Varghese JN, Garrett TPJ, Colman PM, Chen L, Hoj PB, Fincher GB (1994) Three-dimensional structures of two plant beta-glucan endohydrolases with distinct substrate specificities. Proceedings of the National Academy of Sciences of the United States of America 91, 2785–2789.
| Three-dimensional structures of two plant beta-glucan endohydrolases with distinct substrate specificities.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2cXksFaqu7Y%3D&md5=618af241d68705d62ba35120a233245dCAS |
Ward ER, Uknes SJ, Williams SC, Dincher SS, Wiederhold DL, Alexander DC, Ahl-Goy P, Métraux J-P, Ryals JA (1991) Coordinate gene activity in response to agents that induce systemic acquired resistance. The Plant Cell 3, 1085–1094.
Wu CT, Bradford KJ (2003) Class I chitinase and β-1,3-glucanase are differentially regulated by wounding, methyl jasmonate, ethylene, and gibberellin in tomato seeds and leaves. Plant Physiology 133, 263–273.
| Class I chitinase and β-1,3-glucanase are differentially regulated by wounding, methyl jasmonate, ethylene, and gibberellin in tomato seeds and leaves.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXntlaitbk%3D&md5=3df00c91271388d59a16f25ad5cd56bcCAS |
Yamaguchi T, Yamada A, Hong N, Ogawa T, Ishii T, Shibuya N (2000) Differences in the recognition of glucan elicitors signals between rice and soybean: β-glucan fragments from the rice blast disease fungus Pyricularia oryzae that elicit phytoalexin biosynthesis in suspension-cultured rice cells. The Plant Cell 12, 817–826.
