Preliminary characterisation of two early meiotic wheat proteins after identification through 2D gel electrophoresis proteomics
Kelvin H. P. Khoo A , Amanda J. Able A , Timothy K. Chataway B and Jason A. Able A C
+ Author Affiliations
- Author Affiliations
A School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, Waite Campus, PMB1, Glen Osmond, SA 5064, Australia.
B Proteomics Laboratory, School of Medicine, Flinders University, Bedford Park, SA 5042, Australia.
C Corresponding author. Email: jason.able@adelaide.edu.au
Functional Plant Biology 39(3) 222-235 https://doi.org/10.1071/FP11253
Submitted: 11 November 2011 Accepted: 24 December 2011 Published: 14 March 2012
Abstract
Various genetic-based approaches including mutant population screens, microarray analyses, cloning and transgenesis have broadened our knowledge of gene function during meiosis in plants. Nonetheless, these genetic tools are not without inherent limitations. One alternative approach to studying plant meiosis, especially in polyploids such as Triticum aestivum L. (bread wheat), is proteomics. However, protein-based approaches using proteomics have seldom been described, with only two attempts at studying early plant meiosis reported. Here, we report the investigation of early bread wheat meiosis using proteomics. Five differentially expressed protein spots were identified using 2D gel electrophoresis (2DGE) on protein extracts from four pooled stages of meiosis and three genotypes (Chinese Spring wild-type, ph1b and ph2a wheat mutant lines). Tandem mass spectrometry (MS/MS) identification of peptides from these protein spots led to the isolation and characterisation of the full-length clones of a wheat Speckle-type POZ protein, an SF21-like protein and HSP70, and a partial coding sequence of a hexose transporter. Significantly, the putative functions of the Speckle-type POZ protein and HSP70 were confirmed using in vitro DNA binding assays. Through the use of a 2DGE proteomics approach, we show that proteomics is a viable alternative to genetic-based approaches when studying meiosis in wheat. More significantly, we report a potential role for a Speckle-type POZ protein and a HSP70 in chromosome pairing during the early stages of meiosis in bread wheat.
Additional keywords: chromosome pairing, meiosis, HSP70, Speckle-type POZ protein, Triticum aestivum.
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