Generation and scavenging of reactive oxygen species in wheat flag leaves under combined shading and waterlogging stress
Huawei Li A , Jian Cai A , Fulai Liu B , Dong Jiang A C , Tingbo Dai A and Weixing Cao A
+ Author Affiliations
- Author Affiliations
A Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture, Hi-Tech Key Laboratory of Information Agriculture, Jiangsu Province, Nanjing Agricultural University, Nanjing 210095, China.
B University of Copenhagen, Faculty of Life Sciences, Department of Agriculture and Ecology, Højbakkegaard Allé 13, DK-2630 Taastrup, Denmark.
C Corresponding author. Email: jiangd@njau.edu.cn
Functional Plant Biology 39(1) 71-81 https://doi.org/10.1071/FP11165
Submitted: 27 July 2011 Accepted: 22 September 2011 Published: 7 November 2011
Abstract
Wheat (Triticum aestivum L.) plants were subjected to combined waterlogging and shading (WS) at 0–7, 8–15, 16–23 and 24–31 days after anthesis (DAA). WS at 0–7, 8–15, 16–23 and 24–31 DAA caused a yield loss of 17.18%, 14.98%, 7.93% and 7.05%, respectively. These losses were related to reductions in post-anthesis photoassimilate accumulation and 1000-kernel weight. WS reduced net photosynthetic rate (Pn), the maximum efficiency of PSII photochemistry under dark adaptation, actual photosynthetic efficiency and the photochemical quenching coefficient, but increased the quantum yield of quenching. WS caused enhanced concentrations of malondialdehyde and H2O2, and an increased superoxide anion release. Superoxide dismutase and catalase activity were stimulated at 4 days after the onset of WS at 0–7 and 8–15 DAA, but decreased at 8 days after the onset of WS at 0–7, 8–15 and 16–23 DAA. Ascorbate peroxidase, glutathione reductase, dehydroascorbate reductase and monodehydroascorbate reductase activity increased during 0–8 days after the onset of WS at 0–7, 8–15 and 16–23 DAA. At 16–24 DAA, Pn, the level of reactive oxygen species and activity of the antioxidative enzymes fully recovered in plants subjected to WS at 0–7 DAA, but only partially recovered under WS at 8–15 DAA. Expression of the photosythesis-responsive genes RcaB and Cab, and the antioxidative enzyme-related genes Mn-SOD, Cu/Zn-SOD, CAT and GR were consistent with the performance of Pn and the activity of the antioxidative enzymes.
Additional keywords: antioxidative enzymes, chloroplast, photosynthesis.
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