Production of small starch granules by expression of a tandem-repeat of a family 20 starch-binding domain (SBD3-SBD5) in an amylose-free potato genetic background
Farhad Nazarian-Firouzabadi A C , Luisa M. Trindade B and Richard G. F. Visser B
+ Author Affiliations
- Author Affiliations
A Agronomy and Plant Breeding Group, Faculty of Agriculture, Lorestan University, PO Box 465, Khorramabad, Iran.
B Wageningen UR Plant Breeding, Wageningen University, PO Box 386, 6700 AJ Wageningen, The Netherlands.
C Corresponding author. Email: nazarian.f@lu.ac.ir
Functional Plant Biology 39(2) 146-155 https://doi.org/10.1071/FP11150
Submitted: 30 June 2011 Accepted: 24 November 2011 Published: 12 January 2012
Abstract
Starch exists typically as semicrystalline granules of varying size. Granule size plays an important role for many industrial starch applications. Microbial non-catalytic starch binding domains (SBD) exhibit an affinity for starch granules on their own. Three different constructs were introduced in the amylose-free potato cultivar (Solanum tuberosum L. cv. amf) to investigate whether it is possible to produce smaller starch granules by an engineered, high-affinity, tandem-repeats of a family 20 starch-binding domain (SBD3, SBD4 and SBD5). A significant reduction in the size of starch granule was achieved in transgenic potato plants. Furthermore, it was shown that the SBDn expression can affect physical processes underlying granule assembly and the poorly understood granule formation. Expression of multiple linked SBDs resulted in amalgamated starch granules that consisted of many smaller granules. No significant alterations were observed with regard to rheological properties of starch granules.
Additional keywords: amyloplasts, carbohydrate binding module, potato tuber expression, Solanum tuberosum, starch binding domain, starch properties.
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