Identification of chromosomes controlling abscisic acid responsiveness and transcript accumulation of Cor–Lea genes in common wheat seedlings
Julio C. M. Iehisa A , Yumeto Kurahashi A and Shigeo Takumi A B
+ Author Affiliations
- Author Affiliations
A Laboratory of Plant Genetics, Graduate School of Agricultural Science, Kobe University, Rokkodai-cho 1-1, Nada-ku, Kobe 657-8501, Japan.
B Corresponding author. Email: takumi@kobe-u.ac.jp
Functional Plant Biology 38(10) 758-766 https://doi.org/10.1071/FP11092
Submitted: 15 April 2011 Accepted: 18 July 2011 Published: 16 September 2011
Abstract
Abiotic stresses, such as cold, drought or high salinity, seriously affect plant growth and reduce yield in crop species including common wheat (Triticum aestivum L.). The phytohormone ABA plays important roles in plant adaptation to abiotic stress. We compared responsiveness to exogenous ABA, based on root growth inhibition by ABA, among three common wheat cultivars. Seedlings of the cultivars Cheyenne (Cnn) and Hope showed higher ABA responsiveness and higher levels of Cor (cold-responsive)–Lea (late embryogenesis abundant) gene expression than seedlings of Chinese Spring (CS). The chromosomes involved in the regulation of ABA responsiveness and Cor–Lea expression were identified using chromosome substitution lines, in which a chromosome pair of CS was substituted for the corresponding homologous pair of Cnn or Hope. In the CS–Cnn substitution lines, chromosomes 3A, 5A, 5D and 7A increased the ABA responsiveness of CS. Chromosomes 3A and 5A were also involved in the regulation of Cor–Lea gene expression and stomatal response during leaf dehydration. Substitution of CS chromosomes 3A or 5A with the respective homologous pair from Hope also enhanced ABA responsiveness and Cor–Lea expression. In addition, the factors present on chromosomes 4D and 7B of highly responsive cultivars increased Wrab17 expression but had little or no effect on ABA responsiveness. Cultivar differences in ABA responsiveness appear to be determined by genes present on these specific chromosomes in common wheat.
Additional keywords: abiotic stress, chromosome substitution line, drought tolerance.
References
Cattivelli L, Baldi P, Crosatti C, Di Fonzo N, Faccioli P, Grossi M, Mastrangelo AM, Pecchiolini N, Stanca AM (2002) Chromosome regions and stress-related sequences involved in resistance to abiotic stress in Triticeae. Plant Molecular Biology 48, 649–665.| Chromosome regions and stress-related sequences involved in resistance to abiotic stress in Triticeae.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XjsFWrt74%3D&md5=41b02673d6880cbad09420798741bd8fCAS |
Dubcovsky J, Luo MC, Dvorak J (1995) Linkage relationships among stress-induced genes in wheat. Theoretical and Applied Genetics 91, 795–801.
| Linkage relationships among stress-induced genes in wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXpvFyqsr4%3D&md5=d68f505a2c1d7a09df01954a8a76457fCAS |
Egawa C, Kobayashi F, Ishibashi M, Nakamura T, Nakamura C, Takumi S (2006) Differential regulation of transcript accumulation and alternative splicing of a DREB2 homolog under abiotic stress conditions in common wheat. Genes & Genetic Systems 81, 77–91.
| Differential regulation of transcript accumulation and alternative splicing of a DREB2 homolog under abiotic stress conditions in common wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XosValt7k%3D&md5=1fcc82a0bfa449488b79011cc97874deCAS |
Finkelstein RR, Gampala SS, Rock CD (2002) Abscisic acid signaling in seeds and seedlings. The Plant Cell 14, S15–S45.
Fujii H, Chinnusamy V, Rogrigues A, Rubio S, Antoni R, Park SY, Cutler SR, Sheen J, Rodriguez PL, Zhu JK (2009) In vitro reconstitution of an abscisic acid signalling pathway. Nature 462, 660–664.
| In vitro reconstitution of an abscisic acid signalling pathway.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhsVentLnI&md5=0ad6149d2c4dfb612da5f89d9cfd5e43CAS |
Groos C, Gay G, Perretant MR, Gervais L, Bernard M, Dedryver F, Charmet G (2002) Study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a white × red grain bread-wheat cross. Theoretical and Applied Genetics 104, 39–47.
| Study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a white × red grain bread-wheat cross.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XitFyjtL4%3D&md5=fdc3da9dcfa1f3e899da5c8e303fa7f1CAS |
Johnson RR, Wagner RL, Verhey SD, Walker-Simmons MK (2002) The abscisic acid-responsive kinase PKABA1 interacts with a seed-specific abscisic acid response element-binding factor, TaABF, and phosphorylates TaABF peptide sequences. Plant Physiology 130, 837–846.
| The abscisic acid-responsive kinase PKABA1 interacts with a seed-specific abscisic acid response element-binding factor, TaABF, and phosphorylates TaABF peptide sequences.Crossref | GoogleScholarGoogle Scholar |
Kobayashi F, Takumi S, Nakata M, Ohno R, Nakamura T, Nakamura C (2004) Comparative study of the expression profiles of the Cor/Lea gene family in two wheat cultivars with contrasting levels of freezing tolerance. Physiologia Plantarum 120, 585–594.
| Comparative study of the expression profiles of the Cor/Lea gene family in two wheat cultivars with contrasting levels of freezing tolerance.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXjtFWmu7w%3D&md5=10497419154d271196b54303d246fb24CAS |
Kobayashi F, Takumi S, Egawa C, Ishibashi M, Nakamura C (2006) Expression patterns of low temperature responsive genes in a dominant ABA-less-sensitive mutant line of common wheat. Physiologia Plantarum 127, 612–623.
| Expression patterns of low temperature responsive genes in a dominant ABA-less-sensitive mutant line of common wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XovFGhurc%3D&md5=1783df678e31b3658668787707eed648CAS |
Kobayashi F, Ishibashi M, Takumi S (2008a) Transcriptional activation of Cor/Lea genes and increase in abiotic stress tolerance through expression of a wheat DREB2 homolog in transgenic tobacco. Transgenic Research 17, 755–767.
| Transcriptional activation of Cor/Lea genes and increase in abiotic stress tolerance through expression of a wheat DREB2 homolog in transgenic tobacco.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtValt7jJ&md5=9352ef590d1c85f74366d44cc4b0d08bCAS |
Kobayashi F, Maeta E, Terashima A, Kawaura K, Ogihara Y, Takumi S (2008b) Development of abiotic stress tolerance via bZIP-type transcription factor LIP19 in common wheat. Journal of Experimental Botany 59, 891–905.
| Development of abiotic stress tolerance via bZIP-type transcription factor LIP19 in common wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXjs1KhtLY%3D&md5=d1b99d1daeeec4efabcadf629fc9bd9bCAS |
Kobayashi F, Maeta E, Terashima A, Takumi S (2008c) Positive role of a wheat HvABI5 ortholog in abiotic stress response of seedlings. Physiologia Plantarum 134, 74–86.
| Positive role of a wheat HvABI5 ortholog in abiotic stress response of seedlings.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtFChtbrN&md5=acc1062b703356a2f3c8b1ca8a659d10CAS |
Kobayashi F, Takumi S, Nakamura C (2008d) Increased freezing tolerance in an ABA-hypersensitive mutant of common wheat. Journal of Plant Physiology 165, 224–232.
| Increased freezing tolerance in an ABA-hypersensitive mutant of common wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXisFSmu7Y%3D&md5=2e766537533c197ed9fc5d6b8e83ecc6CAS |
Kobayashi F, Takumi S, Handa H (2010) Identification of quantitative trait loci for ABA responsiveness at the seedling stage associated with ABA-regulated gene expression in common wheat. Theoretical and Applied Genetics 121, 629–641.
| Identification of quantitative trait loci for ABA responsiveness at the seedling stage associated with ABA-regulated gene expression in common wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXpt12msbY%3D&md5=26701960ffc4fee268660cb9a0fcc350CAS |
Kosová K, Vitámvás P, Prášil IT (2011) Expression of dehydrins in wheat and barley under different temperatures. Plant Science 180, 46–52.
| Expression of dehydrins in wheat and barley under different temperatures.Crossref | GoogleScholarGoogle Scholar |
Kulwal PL, Kumar N, Gaur A, Khurana P, Khurana JP, Tyagi AK, Balyan HS, Gupta PK (2005) Mapping of a major QTL for pre-harvest sprouting tolerance on chromosome 3A in bread wheat. Theoretical and Applied Genetics 111, 1052–1059.
| Mapping of a major QTL for pre-harvest sprouting tolerance on chromosome 3A in bread wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtV2ksbjN&md5=352c81f2e11f253b57ae9498e6c4428dCAS |
Mao X, Zhang H, Tian S, Chang X, Jing R (2010) TaSnRK2.4, an SNF1-type serine/threonine protein kinase of wheat (Triticum aestivum L.), confers enhanced multistress tolerance in Arabidopsis. Journal of Experimental Botany 61, 683–696.
| TaSnRK2.4, an SNF1-type serine/threonine protein kinase of wheat (Triticum aestivum L.), confers enhanced multistress tolerance in Arabidopsis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhsVSrtLk%3D&md5=f2a781e702c3bd5f5188911def256eecCAS |
McKibbin RS, Wilkinson MD, Bailey PC, Flintham JE, Andrew LM, Lazzeri PA, Gale MD, Lenton JR, Holdsworth MJ (2002) Transcripts of Vp-1 homeologues are misspliced in modern wheat and ancestral species. Proceedings of the National Academy of Sciences of the United States of America 99, 10203–10208.
| Transcripts of Vp-1 homeologues are misspliced in modern wheat and ancestral species.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XlslKgur0%3D&md5=10a5b4a0911f28a584eb0145da79819bCAS |
Nakamura S, Toyama T (2001) Isolation of a VP1 homologue from wheat and analysis of its expression in embryos of dormant and non-dormant cultivars. Journal of Experimental Botany 52, 875–876.
| Isolation of a VP1 homologue from wheat and analysis of its expression in embryos of dormant and non-dormant cultivars.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXltVejsL0%3D&md5=e279c60ace56c2b3e74d87428ab6c360CAS |
Nakamura S, Komatsuda T, Miura H (2007) Mapping diploid wheat homologues of Arabidopsis seed ABA signaling genes and QTLs for seed dormancy. Theoretical and Applied Genetics 114, 1129–1139.
| Mapping diploid wheat homologues of Arabidopsis seed ABA signaling genes and QTLs for seed dormancy.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXkslWnsrw%3D&md5=17f0c80d07d7ebf5d11a685c59525156CAS |
Nakashima K, Fujita Y, Kanamori N, Katagiri T, Umezawa T, Kidokoro S, Maruyama K, Yoshida T, Ishiyama K, Kobayashi M, Shinozaki K, Yamaguchi-Shinozaki K (2009) Three Arabidopsis SnRK2 protein kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3, involved in ABA signaling are essential for the control of seed development and dormancy. Plant & Cell Physiology 50, 1345–1363.
| Three Arabidopsis SnRK2 protein kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3, involved in ABA signaling are essential for the control of seed development and dormancy.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXovVCitbw%3D&md5=07c28a01eb2c1a63f53ec4afeece2d3dCAS |
Ohno R, Takumi S, Nakamura C (2003) Kinetics of transcript and protein accumulation of a low-molecular-weight wheat LEA D-11 dehydrin in response to low temperature. Journal of Plant Physiology 160, 193–200.
| Kinetics of transcript and protein accumulation of a low-molecular-weight wheat LEA D-11 dehydrin in response to low temperature.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXivVWgsbo%3D&md5=3944e41e1d2fd343043d40002caa39f5CAS |
Osa M, Kato K, Mori M, Shindo C, Torada A, Miura H (2003) Mapping QTLs for seed dormancy and the Vp1 homologue on chromosome 3A in wheat. Theoretical and Applied Genetics 106, 1491–1496.
Quarrie SA, Gulli M, Calestani C, Steed A, Marmiroli N (1994) Location of a gene regulating drought-induced abscisic acid production on the long arm of chromosome 5A of wheat. Theoretical and Applied Genetics 89, 794–800.
| Location of a gene regulating drought-induced abscisic acid production on the long arm of chromosome 5A of wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXjsF2jtbw%3D&md5=3ad5e869f24f0d7fc3a8df75674e76f9CAS |
Raghavendra AS, Gonugunta VK, Christmann A, Grill E (2010) ABA perception and signalling. Trends in Plant Science 15, 395–401.
| ABA perception and signalling.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXosVWmsbc%3D&md5=95bcbef3d2e99359481b292da2f6d4aeCAS |
Shen YG, Zhang WK, He SJ, Zhang JS, Liu Q, Chen SY (2003) An EREBP/AP2-type protein in Triticum aestivum was a DRE-binding transcription factor induced by cold, dehydration and ABA stress. Theoretical and Applied Genetics 106, 923–930.
Sutka J (1981) Genetic studies of frost resistance in wheat. Theoretical and Applied Genetics 59, 145–152.
| Genetic studies of frost resistance in wheat.Crossref | GoogleScholarGoogle Scholar |
Szalai G, Janda T, Páldi E, Dubacq JP (2001) Changes in the fatty acid unsaturation after hardening in wheat chromosome substitution lines with different cold tolerane. Journal of Plant Physiology 158, 663–666.
| Changes in the fatty acid unsaturation after hardening in wheat chromosome substitution lines with different cold tolerane.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXks1Git78%3D&md5=5738bc0f9d98e26d82c695876ea90f8bCAS |
Thomashow MF (1999) Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. Annual Review of Plant Physiology and Plant Molecular Biology 50, 571–599.
| Plant cold acclimation: freezing tolerance genes and regulatory mechanisms.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXkt1yktrw%3D&md5=1ec54f0365830fcb6a360ed90c0c0fe9CAS |
Yamaguchi-Shinozaki K, Shinozaki K (2006) Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annual Review of Plant Biology 57, 781–803.
| Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XosVKhtL8%3D&md5=2cca184a6077b624d0ed27f8015cad98CAS |
