Two measures of leaf capacitance: insights into the water transport pathway and hydraulic conductance in leaves
Chris J. Blackman A B and Tim J. Brodribb A
+ Author Affiliations
- Author Affiliations
A School of Plant Science, University of Tasmania, Private Bag 55, Hobart, Tas. 7001, Australia.
B Corresponding author. Email: cblackma@utas.edu.au
Functional Plant Biology 38(2) 118-126 https://doi.org/10.1071/FP10183
Submitted: 2 September 2010 Accepted: 14 November 2010 Published: 1 February 2011
Abstract
The efficiency and stress tolerance of leaf water transport are key indicators of plant function, but our ability to assess these processes is constrained by gaps in our understanding of the water transport pathway in leaves. A major challenge is to understand how different pools of water in leaves are connected to the transpiration stream and, hence, determine leaf capacitance (Cleaf) to short- and medium-term fluctuations in transpiration. Here, we examine variation across an anatomically and phylogenetically diverse group of woody angiosperms in two measures of Cleaf assumed to represent bulk-leaf capacitance (Cbulk) and the capacitance of leaf tissues that influence dynamic changes in leaf hydration (Cdyn). Among species, Cbulk was significantly correlated with leaf mass per unit area, whereas Cdyn was independently related to leaf lignin content (%) and the saturated mass of leaf water per unit dry weight. Dynamic and steady-state measurements of leaf hydraulic conductance (Kleaf) agreed if Cdyn was used rather than Cbulk, suggesting that the leaf tissue in some species is hydraulically compartmentalised and that only a proportion of total leaf water is hydraulically well connected to the transpiration stream. These results indicate that leaf rehydration kinetics can accurately measure Kleaf with knowledge of the capacitance of the hydraulic pathway.
Additional keywords: hydraulic compartmentalisation, leaf dry mass, leaf hydraulic conductance, leaf water content, rehydration kinetics.
References
Aasamaa K, Sober A, Rahi M (2001) Leaf anatomical characteristics associated with shoot hydraulic conductance, stomatal conductance and stomatal sensitivity to changes of leaf water status in temperate deciduous trees. Australian Journal of Plant Physiology 28, 765–774.| Leaf anatomical characteristics associated with shoot hydraulic conductance, stomatal conductance and stomatal sensitivity to changes of leaf water status in temperate deciduous trees.Crossref | GoogleScholarGoogle Scholar |
Blackman CJ, Brodribb TJ, Jordan GJ (2009) Leaf hydraulics and drought stress: response, recovery and survivorship in four woody temperate plant species. Plant, Cell & Environment 32, 1584–1595.
| Leaf hydraulics and drought stress: response, recovery and survivorship in four woody temperate plant species.Crossref | GoogleScholarGoogle Scholar | 19627564PubMed |
Blackman CJ, Brodribb T, Jordan GJ (2010) Leaf hydraulic vulnerability is related to conduit dimensions and drought resistance across a diverse range of woody angiosperms. New Phytologist 188, 1113–1123.
| Leaf hydraulic vulnerability is related to conduit dimensions and drought resistance across a diverse range of woody angiosperms.Crossref | GoogleScholarGoogle Scholar | 20738785PubMed |
Boyer JS (1974) Water transport in plants: mechanisms of apparent changes in resistance during absorption. Planta 175, 374–379.
Brodribb TJ, Cochard H (2009) Hydraulic failure defines the recovery and point of death in water-stressed conifers. Plant Physiology 149, 575–584.
| Hydraulic failure defines the recovery and point of death in water-stressed conifers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXjt1Wqtb4%3D&md5=a54d09c466219abe3e72e82b73616c84CAS | 19011001PubMed |
Brodribb TJ, Feild TS (2010) Leaf hydraulic evolution led a surge in leaf photosynthetic capacity during early angiosperm diversification. Ecology Letters 13, 175–183.
| Leaf hydraulic evolution led a surge in leaf photosynthetic capacity during early angiosperm diversification.Crossref | GoogleScholarGoogle Scholar | 19968696PubMed |
Brodribb TJ, Holbrook NM (2003) Stomatal closure during leaf dehydration, correlation with other leaf physiological traits. Plant Physiology 132, 2166–2173.
| Stomatal closure during leaf dehydration, correlation with other leaf physiological traits.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXmsVantbc%3D&md5=1605ed6dfa36fea1d2d7e19e212a217fCAS | 12913171PubMed |
Brodribb TJ, Holbrook NM (2006) Declining hydraulic efficiency as transpiring leaves desiccate: two types of response. Plant, Cell & Environment 29, 2205–2215.
| Declining hydraulic efficiency as transpiring leaves desiccate: two types of response.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtVaitA%3D%3D&md5=a8f2d6b8c0b1f5db966b02fa94e8c688CAS | 17081253PubMed |
Brodribb TJ, Holbrook NM, Zwieniecki MA, Palma B (2005) Leaf hydraulic capacity in ferns, conifers and angiosperms: impacts on photosynthetic maxima. New Phytologist 165, 839–846.
| Leaf hydraulic capacity in ferns, conifers and angiosperms: impacts on photosynthetic maxima.Crossref | GoogleScholarGoogle Scholar | 15720695PubMed |
Brodribb TJ, Feild TS, Jordan GJ (2007) Leaf maximum photosynthetic rate and venation are linked by hydraulics. Plant Physiology 144, 1890–1898.
| Leaf maximum photosynthetic rate and venation are linked by hydraulics.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXpsVOgs7s%3D&md5=1655bd5a0708a6be957e2e90726baa1eCAS | 17556506PubMed |
Brodribb TJ, Feild TS, Sack L (2010) Viewing leaf structure and evolution from a hydraulic perspective. Functional Plant Biology 37, 488–498.
| Viewing leaf structure and evolution from a hydraulic perspective.Crossref | GoogleScholarGoogle Scholar |
Hao GY, Hoffmann WA, Scholz FG, Bucci SJ, Meinzer FC, Franco AC, Cao KF, Goldstein G (2008) Stem and leaf hydraulics of congeneric tree species from adjacent tropical savanna and forest ecosystems. Oecologia 155, 405–415.
| Stem and leaf hydraulics of congeneric tree species from adjacent tropical savanna and forest ecosystems.Crossref | GoogleScholarGoogle Scholar | 18049826PubMed |
Hatfield R, Fukushima RS (2005) Can lignin be accurately measured? Crop Science 45, 832–839.
| Can lignin be accurately measured?Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXks1Wrurk%3D&md5=159ecbea986eabf2d7d79c13c603efa5CAS |
Kolb KJ, Sperry JS, Lamont BB (1996) A method for measuring xylem hydraulic conductance and embolism in entire root and shoot systems. Journal of Experimental Botany 47, 1805–1810.
| A method for measuring xylem hydraulic conductance and embolism in entire root and shoot systems.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXjsFOqtA%3D%3D&md5=da097532581134474e302a19afb7e9bbCAS |
Lamont BB, Lamont HC (2000) Utilizable water in leaves of 8 arid species as derived from pressure–volume curves and chlorophyll fluorescence. Physiologia Plantarum 110, 64–71.
| Utilizable water in leaves of 8 arid species as derived from pressure–volume curves and chlorophyll fluorescence.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXms1Cqu7w%3D&md5=51d52369e21e55f95f9acd689520dce7CAS |
Nardini A, Gortan E, Salleo S (2005) Hydraulic efficiency of the leaf venation system in sun- and shade-adapted species. Functional Plant Biology 32, 953–961.
| Hydraulic efficiency of the leaf venation system in sun- and shade-adapted species.Crossref | GoogleScholarGoogle Scholar |
Niinemets U (1999) Components of leaf dry mass per area – thickness and density – alter leaf photosynthetic capacity in reverse directions in woody plants. New Phytologist 144, 35–47.
| Components of leaf dry mass per area – thickness and density – alter leaf photosynthetic capacity in reverse directions in woody plants.Crossref | GoogleScholarGoogle Scholar |
Sack L, Holbrook NM (2006) Leaf hydraulics. Annual Review of Plant Biology 57, 361–381.
| Leaf hydraulics.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XosVKhtrs%3D&md5=a29ff2260a6d0acbec987a640491d44cCAS | 16669766PubMed |
Sack L, Tyree M (2005) Leaf hydraulics and its implications in plant structure and function. In ‘Vascular transport in plants’. (Eds NM Holbrook, MA Zwieniecki) pp. 93–114. (Elsevier Academic Press: London)
Sack L, Melcher PJ, Zwieniecki MA, Holbrook NM (2002) The hydraulic conductance of the angiosperm leaf lamina: a comparison of three measurement methods. Journal of Experimental Botany 53, 2177–2184.
| The hydraulic conductance of the angiosperm leaf lamina: a comparison of three measurement methods.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38Xpt1KgtLg%3D&md5=c62c0b2424f0d31430335ae2ee2b14d0CAS | 12379784PubMed |
Sack L, Cowan PD, Jaikumar N, Holbrook NM (2003) The ‘hydrology’ of leaves: co-ordination of structure and function in temperate woody species. Plant, Cell & Environment 26, 1343–1356.
| The ‘hydrology’ of leaves: co-ordination of structure and function in temperate woody species.Crossref | GoogleScholarGoogle Scholar |
Salleo S, Nardini A, LoGullo MA (1997) Is sclerophylly of Mediterranean evergreens an adaptation to drought? New Phytologist 135, 603–612.
| Is sclerophylly of Mediterranean evergreens an adaptation to drought?Crossref | GoogleScholarGoogle Scholar |
Shipley B (1995) Structured interspecific determinants of specific leaf-area in 34 species of herbaceous angiosperms. Functional Ecology 9, 312–319.
| Structured interspecific determinants of specific leaf-area in 34 species of herbaceous angiosperms.Crossref | GoogleScholarGoogle Scholar |
Sokal RR, Rohlf FJ (1995) ‘Biometry.’ (WH Freeman & Company: San Francisco)
Tyree MT, Cheung YNS (1977) Resistance to water flow in Fagus grandifolia leaves. Canadian Journal of Botany 55, 2591–2599.
| Resistance to water flow in Fagus grandifolia leaves.Crossref | GoogleScholarGoogle Scholar |
Tyree MT, Hammel HT (1972) Measurement of turgor pressure and water relations of plants by pressure-bomb technique. Journal of Experimental Botany 23, 267–282.
| Measurement of turgor pressure and water relations of plants by pressure-bomb technique.Crossref | GoogleScholarGoogle Scholar |
Tyree MT, Cruiziat P, Benis M, LoGullo MA, Salleo S (1981) The kinetics of rehydration of detached sunflower leaves from different initial water deficits. Plant, Cell & Environment 4, 309–317.
Tyree MT, Sinclair B, Lu P, Granier A (1993) Whole shoot hydraulic resistance in Quercus species measured with a new high-pressure flowmeter. Annales des Sciences Forestieres 50, 417–423.
| Whole shoot hydraulic resistance in Quercus species measured with a new high-pressure flowmeter.Crossref | GoogleScholarGoogle Scholar |
Vendramini F, Diaz S, Gurvich DE, Wilson PJ, Thompson K, Hodgson JG (2002) Leaf traits as indicators of resource-use strategy in floras with succulent species. New Phytologist 154, 147–157.
| Leaf traits as indicators of resource-use strategy in floras with succulent species.Crossref | GoogleScholarGoogle Scholar |
Weatherly P (1963) The pathway of water movement across the root cortex and leaf mesophyll in transpiring plants. In ‘The water relations of plants’. (Eds A Rutter, F Whitehead) pp. 85–100. (Blackwell: London)
Zwieniecki MA, Brodribb TJ, Holbrook NM (2007) Hydraulic design of leaves: insights from rehydration kinetics. Plant, Cell & Environment 30, 910–921.
| Hydraulic design of leaves: insights from rehydration kinetics.Crossref | GoogleScholarGoogle Scholar | 17617819PubMed |
