Two separate UV-B radiation wavelength regions control expression of different molecular markers in Arabidopsis thaliana
Irina Kalbina A , Shaoshan Li A B , Georgi Kalbin A , Lars Olof Björn B C and Åke Strid A DA Department of Science and Örebro Life Science Center, Örebro University, SE-70182 Örebro, Sweden.
B School of Life Science, South China Normal University, Guangzhou 510631, China.
C Lund University, Department of Cell and Organism Biology, Sölvegatan 35, SE-22362 Lund, Sweden.
D Corresponding author. Email: ake.strid@nat.oru.se
Functional Plant Biology 35(3) 222-227 https://doi.org/10.1071/FP07197
Submitted: 21 December 2007 Accepted: 12 March 2008 Published: 23 April 2008
Abstract
Fluence-response curves were obtained at nine wavelengths in the interval 280–360 nm for mRNA transcripts of four molecular markers induced by ultraviolet-B (UV-B) radiation in Arabidopsis thaliana (L.) Heynh.: CHS (encoding chalcone synthase), PDX1.3 (encoding an enzyme involved in formation of pyridoxine), MEB5.2 (encoding a protein with unknown function but which is strongly upregulated by UV-B), and LHCB1*3 (encoding a chlorophyll a/b binding protein). Intact Arabidopsis plants were irradiated for 3 h using a high intensity deuterium radiation source and narrow bandwith filters without supplementary PAR. The results obtained suggest the existence of two distinct UV-B signal responses: one sensitive between 300 and 310 nm and the other sensitive around 280–290 nm. Among the investigated molecular markers, CHS and PDX1.3 were regulated through the chromophore absorbing around 300 nm, whereas MEB5.2 and LHCB1*3 were regulated through the chromophore absorbing at 280–290 nm. The results obtained show that at least two signal transduction pathways exist that regulate gene expression as a result of absorption of UV-B radiation in plants.
Additional keywords: photoreceptor, UV-B irradiation, wavelength dependence.
Acknowledgements
This work was supported by grants to IK from Helge Ax:son Johnson’s foundation and to ÅS from the Carl Trygger foundation, and the Faculty for Medicine, Natural Sciences and Technology at Örebro University. SL was supported by the National Natural Science Foundation of China (NSFC, Project No. 30370126 and No. 30470283). The authors thank Professor Helen Ghiradella (The University at Albany) for linguistic revision. We are also indebted to Professor Jan Lanke (Mathematical Statistics, Lund University).
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