Dehydrins in Lupinus albus: pattern of protein accumulation in response to drought
Carla Pinheiro A E , Maria H. Cruz de Carvalho A B E , Dorothea Bartels C , Cândido Pinto Ricardo A D and M. Manuela Chaves A D FA Instituto de Tecnologia Química e Biológica (ITQB), Av. da Républica, Apartado 127, 2780-901 Oeiras, Portugal.
B Université Paris 12, 61 Avenue du Général de Gaulle, 94010 Créteil cedex, France.
C Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Kirschallee 1, D-53115, Bonn, Germany.
D Instituto Superior de Agronomia (ISA, UTL), Tapada da Ajuda, 1349-017 Lisboa, Portugal.
E These authors contributed equally.
F Corresponding author. Email: mchaves@itqb.unl.pt
Functional Plant Biology 35(1) 85-91 https://doi.org/10.1071/FP07179
Submitted: 23 July 2007 Accepted: 5 November 2007 Published: 25 January 2008
Abstract
Dehydrins (DHNs) are proteins that accumulate abundantly in various plant tissues in response to environmental stresses and during seed maturation, possibly assisting cells in tolerating dehydration. White lupins (Lupinus albus L.) are able to withstand periods of severe water deficit (WD) and previous work suggested that the stem plays a central role as a survival structure. To investigate DHNs involvement in this strategy, we studied tissue specific protein accumulation of a RAB16-like DHN in lupin during a progressive WD and early recovery. Differences were found between leaves, stems and roots. In leaves and roots, the accumulation of the RAB16-like DHN was independent of the water status whereas in the stem (cortex and stele), DHNs were only detected under severe plant WD (stele relative water content, RWC, reduction of 6–7% and cortex RWC reduction of 20%). DHN mRNA analysis by RT–PCR, showed the presence of one DHN mRNA regardless of the tissue or the plant water status.
Additional keyword: drought tolerance.
Acknowledgements
Thanks to Sylvain Verger for technical assistance on the cloning of LaDhn1. CP (BD/16137/98 and SFRH/BPD/14535/2003) and MHCC (SFRH/BPD/5578/2001) acknowledge FCT grants (Portugal). Project POCTI/BIA/227/94/2001 supported the research.
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