Early events in the signalling pathway for the activation of MAPKs in rice roots exposed to nickel
Po-Yu Chen A B , Tsai-Lien Huang A B and Hao-Jen Huang A CA Department of Life Sciences, National Cheng Kung University, No. 1 University Road, 701 Tainan, Taiwan, ROC.
B These authors contributed equally to this article.
C Corresponding author. Email: haojen@mail.ncku.edu.tw
Functional Plant Biology 34(11) 995-1001 https://doi.org/10.1071/FP07163
Submitted: 25 June 2007 Accepted: 19 July 2007 Published: 1 November 2007
Abstract
It is well known that small quantities of nickel (Ni) are essential for plant species, and higher concentrations of Ni retard plant growth. However, the molecular mechanisms responsible for the regulation of plant growth by Ni are not well understood. The aim of this study is to investigate the early signalling pathways activated by Ni on rice (Oryza sativa L.) root. We showed that Ni elicited a remarkable increase in myelin basic protein (MBP) kinase activities. By immunoblot and immunoprecipitation analyses, it is suggested that Ni-activated 40- and 42-kDa MBP kinases are mitogen-activated protein kinases (MAPKs). Pretreatment of rice roots with the antioxidant, glutathione (GSH), the phospholipase D (PLD) inhibitor, n-butanol, and the calmodulin and CDPK antagonist and W7 inhibited Ni-induced MAPK activation. These results suggest that various signalling components are involved in transduction of the Ni signal in rice roots.
Additional keywords: Ni, phosphorylation.
Acknowledgements
This work was supported by research grants from the National Science Council (NSC 95–2311-B-006–002; NSC 96–2311-B-006–001) and the Ministry of Education of the Republic of China (‘Landmark Project Grant’ for NCKU’s ‘Top-University Project’, B024).
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