Comparison of the expression profiles of susceptible and resistant Eucalyptus grandis exposed to Puccinia psidii Winter using SAGE
David H. Moon A , Guillermo R. Salvatierra A , Danielle G. G. Caldas A , Mayra C. C. Gallo de Carvalho A , Raphael T. Carneiro A , Lívia M. Franceschini A , Shinitiro Oda B and Carlos A. Labate A CA Laboratório Max Feffer de Genética de Plantas, Departamento de Genética, Escola Superior de Agricultura ‘Luiz de Queiroz’, Universidade de São Paulo, Piracicaba-SP, Brasil.
B Suzano Papel e Celulose, Av. Brigadeiro Faria Lima n° 1355, 8° andar, CEP 01452-919, São Paulo-SP, Brasil.
C Corresponding author. Email: calabate@esalq.usp.br
Functional Plant Biology 34(11) 1010-1018 https://doi.org/10.1071/FP07094
Submitted: 17 April 2007 Accepted: 18 September 2007 Published: 1 November 2007
Abstract
Eucalyptus grandis Hill ex Maiden and its hybrids are commonly planted by the Brazilian pulp and paper industry, but they are the most susceptible to the neotropical rust disease caused by Puccinia psidii Winter. In an initial attempt to understand the mechanisms of resistance, we constructed two contrasting Serial Analysis of Gene Expression (SAGE) libraries using susceptible and resistant individuals from a segregating half-sibling E. grandis population. Using the Z-test we identified tags differentially expressed between the libraries, preferentially 239 in the susceptible and 232 in the resistant type individuals. Using public (Expressed Sequence Tags) EST databases, 40 of the susceptible and 70 of the resistant tags matched ESTs and were annotated. By comparing the type of genes and their expression levels, distinct differences between the libraries were observed. Susceptible plants showed gene expression linked to leaf senescence, generalised stress responses and detoxification, and are apparently incapable of inducing a competent host defence response. On the other hand, resistant plants showed genes upregulated for cellular polarisation, cytoskeleton restructuring, vesicle transport, and cellulose and lignin biosynthesis. In the resistant individuals, evidence for systemic resistance, anti-oxidative responses and a hypersensitive response was also observed, although no R gene was identified.
Additional keywords: cellular polarisation, chitinase, hypersensitive response, senescence.
Acknowledgements
We thank Suzano Papel e Cellulose for the biological material, financial support and scholarships to DHM and LMF. We also thank FAPESP for scholarships to DGGC (Proc. 03/04288–7), CAPES for a scholarship to RTC and CNPq for scholarships to GRS (Proc. 140427/2002–3) and MCCGC (Proc. 140101/2003–9).
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