Isolation and differential expression of β-1,3-glucanase messenger RNAs, SrGLU3 and SrGLU4, following inoculation of Sesbania rostrata
Chi-Te Liu A B , Toshihiro Aono A , Misako Kinoshita A , Hiroki Miwa A , Taichiro Iki A , Kyung-Bum Lee A and Hiroshi Oyaizu AA Laboratory of Plant Functional Biotechnology, Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
B Corresponding author. Email: aericliu@mail.ecc.u-tokyo.ac.jp
Functional Plant Biology 33(11) 983-990 https://doi.org/10.1071/FP06086
Submitted: 11 April 2006 Accepted: 27 June 2006 Published: 1 November 2006
Abstract
We report here the isolation and characterisation of two new β-1,3-glucanase cDNAs, SrGLU3 and SrGLU4, from a tropical legume Sesbania rostrata Bremek. & Oberm., which form N2-fixing nodules on the stem after infection by Azorhizobium caulinodans. SrGLU3 was characterised as being grouped in a branch with tobacco class I β-1,3-glucanases, where the isoforms were reported to be induced by either pathogen infection or ethylene treatment. SrGLU4 was characterised as separate from other classes, and we propose this new branch as a new class (Class VI). The SrGLU3 gene was constitutively expressed in normal stem nodules induced by the wild type strain of A. caulinodans (ORS571), and also even in immature stem nodules induced by a mutant (ORS571-C1), which could not form mature stem-nodules. In contrast, the transcript accumulation of SrGLU4 was hardly detectable in immature nodules inoculated by the ORS571-C1 mutant. We suggest that S. rostrata makes use of SrGLU4 to discriminate between symbionts and non-symbionts (mutants) in developing nodules. We propose the SrGLU4 gene as a new nodulin during nodulation.
Keywords: Azorhizobium caulinodans, β-1,3-glucanase, nodulation, pathogenesis-related (PR) protein, Sesbania rostrata, symbiosis, transcript accumulation.
Acknowledgments
This study is supported by Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN) of Bio-oriented Technology Research Advancement Institution (BRAIN) of Japan. We appreciate Dr Iain McTaggart (Department of Global Agricultural Sciences, The University of Tokyo) for his helpful suggestions and comments on the manuscript.
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