Cryptochrome 2 is involved in betacyanin decomposition induced by blue light in Suaeda salsa
Wang Chang-Quan A B and Liu Tao AA College of Life Science, Shandong University of Technology, Zibo City, Shandong 255049, China.
B Corresponding author. Email: whitewater7006@163.com
Functional Plant Biology 33(7) 697-702 https://doi.org/10.1071/FP06073
Submitted: 3 April 2006 Accepted: 1 May 2006 Published: 3 July 2006
Abstract
Seeds of the halophyte Suaeda salsa (L.) Pall. were cultured in 24 h dark and 14 h blue light / 10 h dark to examine the role of blue light and the blue-light-absorbing photoreceptor cryptochrome 2 (CRY2) in betacyanin accumulation, hypocotyl elongation and cotyledon opening in S. salsa seedlings. Darkness significantly promoted betacyanin accumulation and hypocotyl elongation but inhibited cotyledon opening. Blue light suppressed betacyanin accumulation and hypocotyl elongation but stimulated cotyledon opening. Betacyanin in S. salsa seedlings decomposed with time in blue light. Western blot analysis showed that CRY2 protein accumulated both in hypocotyls and cotyledons of S. salsa seedlings grown in dark, but degraded with time in blue light, which was paralleled by a decrease of tyrosine hydroxylation activity of tyrosinase, a key enzyme involved in the betalain biosynthesis pathway. These results suggest that CRY2 protein mediates betacyanin decomposition via inactivation of tyrosinase in S. salsa seedlings, and the blue-light-dependent degradation of CRY2 protein is crucial to its function.
Keywords: betacyanin, blue light, cryptochrome 2 (CRY2), dark, Suaeda salsa, western blotting.
Acknowledgments
We thank Dr Brian Hambrook for critically reading the manuscript. We are grateful for financial support from Science Foundation of Shandong University of Technology.
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