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RESEARCH ARTICLE
Contents Vol 33(1)

TaNAC69 from the NAC superfamily of transcription factors is up-regulated by abiotic stresses in wheat and recognises two consensus DNA-binding sequences

Gang-Ping Xue A C , Neil I. Bower A B , C. Lynne McIntyre A , George A. Riding B , Kemal Kazan A and Ray Shorter A
+ Author Affiliations
- Author Affiliations

A CSIRO Plant Industry, 306 Carmody Rd, St Lucia, Qld 4067, Australia.

B CSIRO Livestock Industries, 306 Carmody Rd, St Lucia, Qld 4067, Australia.

C Corresponding author. Email: Gang-Ping.Xue@csiro.au

Functional Plant Biology 33(1) 43-57 https://doi.org/10.1071/FP05161
Submitted: 1 July 2005  Accepted: 16 August 2005   Published: 3 January 2006

Abstract

NAC proteins are one of the largest families of plant transcription factors and have recently been implicated in diverse physiological processes. To elucidate their role in gene regulation, we determined the DNA-binding specificity of a drought- and cold-inducible NAC protein, TaNAC69 from wheat, and analysed its homologues from other species. Two consensus DNA-binding sequences (spanning 23–24 bp) of TaNAC69 were identified through binding site selection and both consisted of two half sites. Comprehensive data on the DNA-binding specificity of TaNAC69 were generated through extensive base substitution mutagenesis. TaNAC69 and its homologue in Arabidopsis, NAP, sharing 75% sequence identity in the NAC domain, exhibited similar DNA-binding specificity. TaNAC69 was able to homodimerise through its NAC domain. The NAC domain consists of five conserved subdomains. Subdomain mutation showed that a loss or reduction in TaNAC69 dimerisation capacity was accompanied with abolition or decrease in its DNA-binding activity. These data suggest that all subdomains are necessary to maintain a functional NAC domain structure required for interaction with DNA and dimerisation.

Keywords: Arabidopsis, dimerisation, DNA-binding specificity, drought and cold stress, NAC transcription factor, wheat.


Acknowledgment

The project was supported by the Australian Grains Research and Development Corporation.


References


Aida M, Ishida T, Fukaki H, Fujisawa H, Tasaka M (1997) Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant. The Plant Cell 9, 841–857.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Collinge M, Boller T (2001) Differential induction of two potato genes, Stprx2 and StNAC, in response to infection by Phytophthora infestans and to wounding. Plant Molecular Biology 46, 521–529.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Delessert C, Kazan K, Wilson IW, Straeten DVD, Manners J, Dennis ES, Dolferus R (2005) The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis. The Plant Journal 43, 745–757.
| Crossref | PubMed |
open url image1

Duval M, Hsieh TF, Kim SY, Thomas TL (2002) Molecular characterization of AtNAM: a member of the Arabidopsis thaliana NAC domain superfamily. Plant Molecular Biology 50, 237–248.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Ernst HA, Olsen AN, Skriver K, Larsen S, Leggio LL (2004) Structure of the conserved domain of ANAC, a member of the NAC family of transcription factors. EMBO Reports 5, 297–303.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Fujita M, Fujita Y, Maruyama K, Seki M, Hiratsu K, Ohme-Takagi M, Tran L-SP, Yamaguchi-Shinozaki K, Shinozaki K (2004) A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway. The Plant Journal 39, 863–876.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Hegedus D, Yu M, Baldwin D, Gruber M, Sharpe A, Parkin I, Whitwill S, Lydiate D (2003) Molecular characterization of Brassica napus NAC domain transcriptional activators induced in response to biotic and abiotic stress. Plant Molecular Biology 53, 383–397.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Hibara K, Takada S, Tasaka M (2003) CUC1 gene activates the expression of SAM-related genes to induce adventitious shoot formation. The Plant Journal 36, 687–696.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Kikuchi K, Ueguchi-Tanaka M, Yoshida KT, Nagato Y, Matsusoka M, Hirano HY (2000) Molecular analysis of the NAC gene family in rice. Molecular & General Genetics 262, 1047–1051. open url image1

Marè C, Mazzucotelli E, Crosatti C, Francia E, Stanca AM, Cattivelli L (2004) Hv-WRKY38: a new transcription factor involved in cold- and drought-response in barley. Plant Molecular Biology 55, 399–416.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Nadassy K, Wodak SJ, Janin J (1999) Structural features of protein–nucleic acid recognition sites. Biochemistry 38, 1999–2017.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Niu XP, Helentjaris T, Bate NJ (2002) Maize ABI4 binds coupling element1 in abscisic acid and sugar response genes. The Plant Cell 14, 2565–2575.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Ooka H, Satoh K, Doi K, Nagata T, Otomo Y , et al. (2003) Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana. DNA Research 10, 239–247.
| Crossref | PubMed |
open url image1

Robertson M (2004) Two transcription factors are negative regulators of gibberellin response in the HvSPY-signaling pathway in barley aleurone. Plant Physiology 136, 2747–2761.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Sablowski RWM, Meyerowitz EM (1998) A homolog of NO APICAL MERISTEM is an immediate target of the floral homeotic genes APETALA3 / PISTILLATA. Cell 92, 93–103.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Schwarz E, Oesterhelt D, Reinke H, Beyreuther K, Dimroth P (1988) The sodium ion translocating oxalacetate decarboxylase of Klebsiella pneumoniae. Sequence of the biotin-containing alpha-subunit and relationship to other biotin-containing enzymes. Journal of Biological Chemistry 263, 9640–9645.
| PubMed |
open url image1

Selth LA, Dogra SC, Rasheed MS, Healy H, Randles JW, Rezaian MA (2005) A NAC domain protein interacts with tomato leaf curl virus replication accessory protein and enhances viral replication. The Plant Cell 17, 311–325.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Souer E, van Houwelingen A, Kloos D, Mol J, Koes R (1996) The no apical meristem gene of Petunia is required for pattern formation in embryos and flowers and is expressed at meristem and primordia boundaries. Cell 85, 159–170.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Takada S, Hibara KI, Ishida T, Tasaka M (2001) The CUP-SHAPED COTYLEDON1 gene of Arabidopsis regulates shoot apical meristem formation. Development 128, 1127–1135.
| PubMed |
open url image1

Tran L-SP, Nakashima K, Sakuma Y, Simpson SD, Fujita Y, Maruyama K, Fujita M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2004) Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter. The Plant Cell 16, 2481–2498.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Xie Q, Sanz-Burgos AP, Guo H, Garcia JA, Gutierrez C (1999) GRAB proteins, novel members of the NAC domain family, isolated by their interaction with a geminivirus protein. Plant Molecular Biology 39, 647–656.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Xie Q, Frugis G, Colgan D, Chua NH (2000) Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development. Genes & Development 14, 3024–3036.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Xie Q, Guo HS, Dallman G, Fang SY, Weissman AM, Chua NH (2002) SINAT5 promotes ubiquitin-related degradation of NAC1 to attenuate auxin signals. Nature 419, 167–170.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Xue GP (2002a) An AP2 domain transcription factor HvCBF1 activate expression of cold-responsive genes in barley through interaction with a (G / a)(C / t)CGAC motif. Biochimica et Biophysica Acta 1577, 63–72.
| PubMed |
open url image1

Xue GP (2002b) Characterisation of the DNA-binding profile of barley HvCBF1 using an enzymatic method for rapid, quantitative and high-throughput analysis of the DNA-binding activity. Nucleic Acids Research 30, e77.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Xue GP (2003) The DNA-binding activity of an AP2 transcriptional activator HvCBF2 involved in regulation of low-temperature responsive genes in barley is modulated by temperature. The Plant Journal 33, 373–383.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Xue GP (2005) A CELD-fusion method for rapid determination of the DNA-binding sequence specificity of novel plant DNA-binding proteins. The Plant Journal 41, 638–649.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Xue GP, Loveridge CW (2004) HvDRF1 is involved in abscisic acid-mediated gene regulation in barley and produces two forms of AP2 transcriptional activators, interacting preferably to a CT-rich element. The Plant Journal 37, 326–339.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1