Heat-induced oxidative activity protects suspension-cultured plant cells from low temperature damage
Andrew C. Allan A C , Ratnasiri Maddumage A , Joanne L. Simons A , Samuel O. Neill A B and Ian B. Ferguson AA HortResearch Mt Albert, Private Bag 92 169, Auckland, New Zealand.
B Current address: Nonlinear Dynamics Limited, Tyne House, 26 Side, Newcastle-upon-Tyne, NE1 3JA, UK.
C Corresponding author. Email: aallan@hortresearch.co.nz
Functional Plant Biology 33(1) 67-76 https://doi.org/10.1071/FP05077
Submitted: 1 April 2005 Accepted: 5 August 2005 Published: 3 January 2006
Abstract
A short heat pre-treatment (1 h at 38°C) was found to protect both suspension-cultured apple fruit cells and tobacco cells from cold-induced cell death. Tobacco cells were more sensitive to low temperatures than apple cells, with significant cell death after 48 h at 0 or –2°C. Real-time measurements of H2O2 levels during the heat pre-treatment revealed a substantial burst of this reactive oxygen species in both cell types. Real-time and longer-term measurements also showed a large burst of H2O2 production from tobacco cells, but not apple cells, when exposed to low temperatures. Lower temperatures reduced levels of peroxidase activity (both total and intracellular), with the heat pre-treatment preventing some of the cold-induced reduction of this activity in both apple and tobacco cells. The greater sensitivity to low temperature of the tobacco cells may be related to higher H2O2 production, with the heat treatment maintaining higher peroxidase activity. The lesser sensitivity of the apple cells may be due to the lack of a H2O2 burst and maintenance of peroxidase activity by the heat treatment. These results support a role for oxidative metabolism in the beneficial effects of heat in inducing low temperature tolerance.
Keywords: acclimation, apple (Malus pumila), hydrogen peroxide, low temperature, oxidative stress; tobacco (Nicotiana tabacum).
Acknowledgments
This study was funded by the New Zealand Foundation of Research, Science and Technology (CO6X0205), and the Marsden Fund of New Zealand.
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