The role and the interrelationship of hydrogen peroxide and nitric oxide in the UV-B-induced stomatal closure in broad bean
Jun-Min He A C D , Hua Xu C , Xiao-Ping She C , Xi-Gui Song C and Wen-Ming Zhao AA Center of Bioinformation, School of Life Sciences and Technology,
B Xi’an Jiaotong University, Xi’an 710049, People’s Republic of China.
C School of Life Sciences, Shaanxi Normal University, Xi’an 710062, People’s Republic of China.
D Corresponding author. Email: hejm1965@yahoo.com.cn
Functional Plant Biology 32(3) 237-247 https://doi.org/10.1071/FP04185
Submitted: 10 October 2004 Accepted: 10 February 2005 Published: 5 April 2005
Abstract
Previous studies have showed that UV-B can stimulate closure as well as opening of stomata. However, the mechanism of this complex effect of UV-B is not clear. The purpose of this paper is to investigate the role and the interrelationship of H2O2 and NO in UV-B-induced stomatal closure in broad bean (Vicia faba L.). By epidermal strip bioassay and laser-scanning confocal microscopy, we observed that UV-B-induced stomatal closure could be largely prevented not only by NO scavenger c-PTIO or NO synthase (NOS) inhibitor l-NAME, but also by ascorbic acid (ASC, an important reducing substrate for H2O2 removal) or catalase (CAT, the H2O2 scavenger), and that UV-B-induced NO and H2O2 production in guard cells preceded UV-B-induced stomatal closure. These results indicate that UV-B radiation induces stomatal closure by promoting NO and H2O2 production. In addition, c-PTIO, l-NAME, ASC and CAT treatments could effectively inhibit not only UV-B-induced NO production, but also UV-B-induced H2O2 production. Exogenous H2O2-induced NO production and stomatal closure were partly abolished by c-PTIO and l-NAME. Similarly, exogenous NO donor sodium nitroprusside-induced H2O2 production and stomatal closure were also partly reversed by ASC and CAT. These results show a causal and interdependent relationship between NO and H2O2 during UV-B-regulated stomatal movement. Furthermore, the l-NAME data also indicate that the NO in guard cells of Vicia faba is probably produced by a NOS-like enzyme.
Keywords: UV-B radiation, stomatal closure, nitric oxide, hydrogen peroxide, broad bean (Vicia faba L.).
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