Symbiosis of Acacia auriculiformis and Acacia mangium with mycorrhizal fungi and Bradyrhizobium spp. improves salt tolerance in greenhouse conditions
Diégane Diouf A B F , Robin Duponnois C , Amadou Tidiane Ba A , Marc Neyra B D and Didier Lesueur B EA Département de Biologie Végétale, Université Cheikh Anta Diop, BP 5005, Dakar, Sénégal.
B Laboratoire Commun de Microbiologie, IRD / ISRA / UCAD, BP 1386, Dakar, Sénégal.
C UR 40, Symbioses Tropicales et Méditerranéennes, IRD, 01 BP 182, Ouagadougou, Burkina Faso.
D UR 40, Symbioses Tropicales et Méditerranéennes, IRD, BP 1386, Dakar, Sénégal.
E Forest Department of CIRAD, UPR ‘ETP’, Tropical Soil Biology and Fertility Institute of CIAT, Nairobi, Kenya.
F Corresponding author. Email: diegane.diouf@ird.sn
Functional Plant Biology 32(12) 1143-1152 https://doi.org/10.1071/FP04069
Submitted: 8 April 2004 Accepted: 25 July 2005 Published: 1 December 2005
Abstract
The aim of our work was to assess the growth and mineral nutrition of salt stressed Acacia auriculiformis A. Cunn. ex Benth. and Acacia mangium Willd. seedlings inoculated with a combination of selected microsymbionts (bradyrhizobia and mycorrhizal fungi). Plants were grown in greenhouse conditions in non-sterile soil, irrigated with a saline nutrient solution (0, 50 and 100 mm NaCl). The inoculation combinations consisted of the Bradyrhizobium strain Aust 13c for A. mangium and Aust 11c for A. auriculiformis, an arbuscular mycorrhizal fungus (Glomus intraradices, DAOM 181602) and an ectomycorrhizal fungus (Pisolithus albus, strain COI 007). The inoculation treatments were designed to identify the symbionts that might improve the salt tolerance of both Acacia species. The main effect of salinity was reduced tree growth in both acacias. However, it appeared that, compared with controls, both rhizobial and mycorrhizal inoculation improved the growth of the salt-stressed plants, while inoculation with the ectomycorrhizal fungus strain appeared to have a small effect on their growth and mineral nutrition levels. Endomycorrhizal inoculation combined with rhizobial inoculation usually gave good results. Analysis of foliar proline accumulation confirmed that dual inoculation gave the trees better tolerance to salt stress and suggested that the use of this dual inoculum might be beneficial for inoculation of both Acacia species in soils with moderate salt constraints.
Keywords: Acacia, microsymbionts, proline accumulation, salt stress.
Acknowledgments
The authors are grateful to Leon Biagui and Abdoulaye Sy for technical assistance in the nursery experiments and to Dr Antoine Galiana for helpful discussions on statistical analyses.
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