Mobilisation and distribution of starch and total N in two grapevine cultivars differing in their susceptibility to shedding
Christophe Zapata A , Eliane Deléens B , Sylvain Chaillou C and Christian Magné A DA Université de Reims Champagne-Ardenne, UFR Sciences, Laboratoire de Biologie et Physiologie Végétales, UPRES EA 2069 (URVVC), BP 1039, 51687 Reims Cedex 2, France.
B Institut de Biotechnologie des Plantes, URA CNRS 1128, Université de Paris-Sud, Orsay, France (deceased).
C Institut National Agronomique de Paris Grignon, Laboratoire de Physiologie Végétale, Paris, France.
D Present address: Université de Bretagne Occidentale, LEBHAM / IUEM, EA 3877, Technopole Brest Iroise, 29280 Plouzané, France. Corresponding author. Email: christian.magne@univ-brest.fr
Functional Plant Biology 31(11) 1127-1135 https://doi.org/10.1071/FP04028
Submitted: 5 February 2004 Accepted: 23 September 2004 Published: 18 November 2004
Abstract
As a part of a project aimed at elucidating the causal relationship between reserve mobilisation and the extent of shedding in Vitis vinifera L., we compared storage and fate of carbon (C) and nitrogen (N) reserves in two varieties differing in their susceptibility to fruitlet abscission. Merlot (susceptible) and Pinot Noir (P. Noir, not susceptible) vines were grown in trenches under semi-controlled conditions over a 3-y period after planting. Mobilisation of stored C and N, distribution of reserve materials within the vines and 15N uptake were followed particularly during the spring growth flush and floral development in the third year. At dormancy, starch levels in the perennial tissues (roots, trunk, canes) were higher in Merlot than in P. Noir. During the spring growth flush, starch level decreased markedly in the roots of both cultivars until early bloom. At that time, starch started to accumulate in P. Noir but not in Merlot. Similar variations were found with total N. Accordingly, 15N analysis showed that translocation of storage N to the annual tissues was nearly achieved at early bloom in P. Noir while it continued until pea berry size in Merlot. In parallel, N uptake increased during the spring growth flush, and it was higher in P. Noir than in Merlot. These results indicate that transition between heterotrophic (root) and autotrophic (leaf) mode of nutrient allocation towards the developing inflorescences occurs earlier in P. Noir. Possible consequences are discussed in relation to the susceptibility of each cultivar to shedding.
Keywords: nitrogen uptake, reserve mobilisation, shedding, starch, Vitis vinifera.
Acknowledgements
We thank SA Mumm Perrier-Jouët Vignobles et Recherches, Epernay (France) for funding the PhD grant to C Zapata and the inter-region VVS research network for financial support.
Andersen PE, Brodbeck BV
(1989) Diurnal and temporal changes in the chemical profile of xylem exudate from Vitis rotundifolia L. Physiologia Plantarum 75, 63–70.
Araujo FJ, Williams LE
(1988) Dry matter and nitrogen partitioning and root growth of young field-grown Thompson Seedless grapevines. Vitis 27, 21–32.
Broquedis M,
Lespy-Labaylette P, Bouard J
(1996) Les polyamines et leur effet anti-coulure chez la vigne. Journal International des Sciences de la Vigne et du Vin 30, 55–58.
Buttrose MS
(1970) Fruitfulness in grapevines: the response of different cultivars to light, temperature and day length. Vitis 9, 455–464.
Caspari HW,
Lang A, Alspach P
(1998) Effects of girdling and leaf removal on fruit set and vegetative growth in grape. American Journal of Enology and Viticulture 49, 359–366.
Coïc F, Lesaint L
(1971) Comment assurer une bonne nutrition en eau et en ions minéraux en horticulture. Horticulture Française 8, 11–14.
Conradie WJ
(1980) Seasonal uptake of nutrients by Chenin blanc in sand culture. I. Nitrogen. South African Journal of Enology and Viticulture 1, 59–65.
Conradie WJ
(1983) The uptake and distribution of 15N enriched nitrate by time of application and soil type. South African Journal of Enology and Viticulture 4, 45–47.
Conradie WJ
(1986) Utilization of nitrogen by the grapevine as affected by time of application and soil type. South African Journal of Enology and Viticulture 7, 76–83.
Conradie, WJ (1991). Translocation and storage of nitrogen by grapevines as affected by time of application. In ‘Proceeding of the international symposium on nitrogen in grape and wine’. Seattle, WA. pp. 32–42.
Deléens E,
Cliquet JB, Prioul JL
(1994) Using 13C and 15N label near natural abundance for monitoring carbon and nitrogen partitioning. Australian Journal of Plant Physiology 21, 133–146.
Ebadi A, Coombe BG
(1996) Effect of short-term temperature and shading on fruit set, seed and berry development in model vines of V. vinifera cv. Chardonnay and Shiraz. Australian Journal of Grape and Wine Research 2, 2–9.
Eichhorn KW, Lorenz DH
(1977) Phänologische Entwicklungsstadien der Rebe. Nachrichtenblatt des Deutschen Pflanzenschutzdienstes (Braunschweig) 29, 119–120.
Ewart A, Kliewer WM
(1977) Effects of controlled day and night temperatures and nitrogen on fruit set, ovule fertility, and fruit composition of several wine grape cultivars. American Journal of Enology and Viticulture 28, 88–95.
Fournioux JC
(1997) Influences foliaires sur le développement et la maturation des grappes. Progrès Agricole et Viticole 17, 359–372.
Glad C,
Regnard JL,
Querou Y,
Brun O, Morot-Gaudry JF
(1992) Flux and chemical composition of xylem exudates from Chardonnay grapevines: temporal evolution and effect of recut. American Journal of Enology and Viticulture 43, 275–282.
Gu S,
Lombard PB, Price S
(1996) Effect of shading and nitrogen source on growth, tissue ammonium and nitrate status and inflorescence necrosis in Pinot Noir grapevines. American Journal of Enology and Viticulture 47, 173–180.
Hügelschäffer P
(1990) ‘Reaktionen von Reben (Vitis vinifera L.) cv Riesling und Müller-Thurgau auf Sommerschnittbehandlungen.’ PhD thesis
(Universität Giessen:
Germany)
Huglin, P ,
and
Schneider, C (1998).
Jackson DI
(1991) Environmental and hormonal effects on development of early bunchstem necrosis. American Journal of Enology and Viticulture 42, 290–294.
Jean D, Lapointe L
(2001) Limited carbohydrate availability as a potential cause of fruit abortion in Rubus chamaemorus. Physiologia Plantarum 112, 379–387.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Keller M,
Arnink KJ, Hrazdina G
(1998) Interaction of nitrogen availability during bloom and light intensity during veraison. I. Effects on grapevine growth, fruit development and ripening. American Journal of Enology and Viticulture 49, 333–340.
Keller M, Koblet W
(1994) Is carbon starvation rather than excessive nitrogen supply the cause of inflorescence necrosis in Vitis vinifera L.? Vitis 33, 81–86.
Keller M, Koblet W
(1995) Dry matter and leaf area partitioning, bud fertility and second season growth of Vitis vinifera L.: responses to nitrogen supply and limiting irradiance. Vitis 34, 77–83.
Kleeze RA, Smith LJ
(1970) Scion control of genotypic differences in mineral salts accumulation in soyabean seeds. Annals of Botany 34, 183–188.
Kliewer, WM (1991). Methods for determining the nitrogen status of wineyards. In ‘Proceedings of the international symposium on nitrogen in grape and wine’. Seattle, WA. pp. 133–147.
Kriedemann P,
Kliewer W, Harris J
(1970) Leaf age and photosynthesis in Vitis vinifera L. Vitis 9, 97–103.
Langheinrich U, Tischner R
(1991) Vegetative storage proteins in poplar. Plant Physiology 97, 1017–1025.
Loescher WH,
McCamant T, Keller JD
(1990) Carbohydrates reserves, translocation and storage in woody plant roots. HortScience 25, 274–281.
Löhnertz O
(1988) Nährstoffelementaufnahme von Reben im Verlauf eines Vegetationszyklus. Mitteilung Klosterneuburg 38, 124–129.
Merjanian A, Ravaz V
(1930) Sur la coulure de la vigne. Progrès Agricole et Viticole 49, 545–550.
Obbink JG,
McE Alexander DE, Possingham JV
(1973) Use of nitrogen and potassium reserves during growth of grape vine cuttings. Vitis 12, 207–213.
O’Kennedy BT, Titus JS
(1979) Isolation and mobilization of storage proteins from apple shoot bark. Physiologia Plantarum 45, 419–424.
Ollat, N (1992). Nouaison chez Vitis vinifera L. cv Merlot Noir: rôle de l'intensité lumineuse et de la photosynthèse à la floraison. In ‘Piemont Journal of the 4th international symposium on grapevine physiology’. pp. 113–116. (Turin: Italy)
Peacock WL,
Christensen LP, Brodbent FE
(1989) Uptake, storage and utilization of soil-applied nitrogen by ‘Thompson Seedless’ as affected by the time of application. American Journal of Enology and Viticulture 40, 16–20.
Pouget R
(1981) Action de la température sur la différenciation des inflorescences et des fleurs durant les phases de pré-débourrement des bourgeons latents de la vigne. Connaissance de la Vigne et du Vin 15, 65–79.
Rodrigo J,
Hormaza JI, Herrero M
(2000) Ovary starch reserves and flower development in apricot (Prunus armeniaca). Physiologia Plantarum 108, 35–41.
| Crossref | GoogleScholarGoogle Scholar |
Sauter JJ, Van Cleve B
(1994) Storage, mobilization and interrelations of starch, sugars, protein and fat in the ray storage tissue of poplar trees. Trees 8, 297–304.
| Crossref |
Schaller K,
Löhnertz O,
Geiben R, Breit N
(1989) N-Stoffwechsel von Reben: N-und Arginindynamik im Holzkörper der Sorte Müller-Thurgau im Verlauf einer Vegetationsperiode. Wein-Wissenschaften 44, 91–101.
Scholefield PB,
Neales TP, May P
(1978) Carbon balance of the sultana vine (Vitis vinifera L.) and the effects of autumn defoliation by harvest pruning. Australian Journal of Plant Physiology 5, 561–570.
Williams, LE (1991). Vine nitrogen requirements. Utilization of N sources from soils, fertilizers and reserves. In ‘Proceedings of the international symposium on nitrogen in grape and wine’. Seattle, WA. pp. 62–66.
Yang YS,
Hori Y, Ogata R
(1980) Studies on retranslocation of accumulated assimilates in ‘Delaware’ grapevine. II. Retranslocation of assimilates accumulated during the previous growing season. Tohuku Journal of Agricultural Research 31, 109–119.
| Crossref |
Zapata C,
Magné C,
Deléens E,
Brun O,
Audran JC, Chaillou S
(2001) Grapevine culture in trenches. I. Root growth and dry matter partitioning. Australian Journal of Grape and Wine Research 7, 127–131.
Zapata C,
Audran JC, Magné C
(2003) Grapevine culture in trenches. II. Reproductive characteristics and interaction with vegetative growth. Journal International des Sciences de la Vigne et du Vin 37, 85–90.
Zapata C,
Deléens E,
Chaillou S, Magné C
(2004) Partitioning and mobilization of starch and N reserves in grapevine (Vitis vinifera L.). Journal of Plant Physiology 161, 1031–1040.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Zimmermann, MH (1971). Storage, mobilization and circulation of assimilates. In ‘Trees: structure and function’. pp. 307–322. (Springer-Verlag: Berlin)
