Measurement of trace metal influx in plants: a case study with Co
Rob Reid A B and Juhong Liu AA School of Earth and Environmental Sciences, University of Adelaide, Adelaide, SA 5005, Australia.
B Corresponding author; email: robert.reid@adelaide.edu.au
Functional Plant Biology 31(9) 941-947 https://doi.org/10.1071/FP04012
Submitted: 20 January 2004 Accepted: 19 May 2004 Published: 27 September 2004
Abstract
The analysis of transport systems involved in the uptake of trace metals in plants is complicated by technical difficulties associated with measurement of uptake and by the likely presence of multiple transporters with broad specificity. In this study, influx of Co was used to illustrate the problems involved and potential solutions. Issues surrounding kinetic descriptions of transport, multiple uptake systems, specificity of transporters, pH effects and the role of membrane surface charge in determining fluxes are addressed. A list of criteria for validation of flux measurements is provided.
Keywords: mung bean, Nramps, surface potential, transport kinetics, ZIPs.
Acknowledgments
We acknowledge financial assistance from the Australian Research Council.
Demidchik V,
Davenport RJ, Tester M
(2002) Nonselective cation channels in plants. Annual Review of Plant Biology 53, 67–107.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Eide D,
Broderius M,
Fett J, Guerinot ML
(1996) A novel iron-regulated metal transporter from plants identified by functional expression in yeast. Proceedings of the National Academy of Sciences USA 93, 5624–5628.
| Crossref | GoogleScholarGoogle Scholar |
Gage R,
Van Wijngaarden W,
Theuvenet A,
Borst-Pauwels G, Verkleij A
(1985) Inhibition of Rb+ uptake in yeast by Ca2+ is caused by a reduction in the surface potential and not in the Donnan potential of the cell wall. Biochimica et Biophysica Acta 812, 1–8.
| Crossref | GoogleScholarGoogle Scholar |
Grotz N,
Connolly E,
Park W,
Guerinot M, Eide D
(1998) Identification of a family of zinc transporter genes from Arabidopsis that respond to zinc deficiency. Proceedings of the National Academy of Sciences USA 95, 7220–7224.
| Crossref | GoogleScholarGoogle Scholar |
Guerinot ML
(2000) The ZIP family of metal transporters. Biochimica et Biophysica Acta Biomembranes 1465, 190–198.
| Crossref |
Gunshin H,
Mackenzie B,
Berger UV,
Gunshin Y,
Romero MF,
Boron WF,
Nussberger S,
Gollan JL, Hediger MA
(1997) Cloning and characterization of a mammalian proton-coupled metal-ion transporter. Nature 388, 482–488.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Johnson C,
Stout P,
Broyer T, Carlton A
(1957) Comparative chlorine requirements of different plant species. Plant and Soil 8, 337–353.
Kinraide T
(1994) Use of a Gouy–Chapman–Stern model for membrane surface electrical potential to interpret some features of mineral rhizotoxicity. Plant Physiology 106, 1583–1592.
| PubMed |
Kinraide T,
Yermiyahi U, Rytwo G
(1998) Computation of surface electrical potentials of plant cell membranes. Plant Physiology 118, 505–512.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Korshunova Y,
Eide D,
Clark W,
Guerinot ML, Pakrasi H
(1999) The IRT1 protein from Arabidopsis thaliana is a metal transporter with a broad substrate range. Plant Molecular Biology 40, 37–44.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Liu J,
Reid RJ, Smith FA
(1998) Mechanisms of cobalt uptake in plants: Co-60 uptake and distribution in Chara. Physiologia Plantarum 104, 351–356.
| Crossref | GoogleScholarGoogle Scholar |
Macklon A, Sim A
(1990) Cortical cell fluxes of cobalt in roots and transport to shoots of ryegrass seedlings. Physiologia Plantarum 80, 409–416.
| Crossref | GoogleScholarGoogle Scholar |
Parker D, Norvell W, Chaney R
(1995) GEOCHEM-PC: a chemical speciation program for IBM and compatible personal computers. ‘Soil chemical equilibrium and reaction models’. (Eds R Leoppert, A Schwab, S Goldberg)
pp. 253–269. (Soil Science Society of America: Madison, WI)
Pence N,
Larsen P,
Ebbs S,
Letham D,
Lasat M,
Garvin DF,
Eide D, Kochian LV
(2000) The molecular physiology of heavy metal transport in the Zn / Cd hyperaccumulator Thlaspi caerulescens. Proceedings of the National Academy of Sciences USA 97, 4956–4960.
| Crossref | GoogleScholarGoogle Scholar |
Reid R,
Brookes J,
Tester MA, Smith FA
(1996) The mechanism of zinc uptake in plants. Planta 198, 39–45.
| Crossref |
Taylor G,
McDonald-Stephens J,
Hunter D,
Bertsch P,
Elmore D,
Rengel Z, Reid R
(2000) Direct measurement of aluminium uptake and distribution in single cells of Chara corallina. Plant Physiology 123, 987–996.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Thomine S,
Wang RC,
Ward JM,
Crawford NM, Schroeder JI
(2000) Cadmium and iron transport by members of a plant metal transporter family in Arabidopsis with homology to Nramp genes. Proceedings of the National Academy of Sciences USA 97, 4991–4996.
| Crossref | GoogleScholarGoogle Scholar |
Vert G,
Grotz N,
Dédaldéchamp F,
Gaymard F,
Guerinot ML,
Briat J-F, Curie C
(2002) IRT1, an Arabidopsis transporter essential for iron uptake from the soil and for plant growth. The Plant Cell 14, 1223–1233.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Zhang Q,
Smith F,
Sekimoto H, Reid R
(2001) Effect of membrane surface charge on nickel uptake by purified mung bean root protoplasts. Planta 213, 788–793.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
